Academic Journal
BpMYB73 regulates long noncoding RNA BplncW20 to improve drought tolerance by mediating ROS scavenging in Betula platyphylla.
| Τίτλος: | BpMYB73 regulates long noncoding RNA BplncW20 to improve drought tolerance by mediating ROS scavenging in Betula platyphylla. |
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| Συγγραφείς: | Zhao H; School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, State Key Laboratory of Tropical Crop Breeding, Hainan University, Sanya, 572025, China., Jia Y; School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, State Key Laboratory of Tropical Crop Breeding, Hainan University, Sanya, 572025, China.; National Key Laboratory for Development and Utilization of Forest Food Resources, College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, 311300, China., Niu Y; National Key Laboratory for Development and Utilization of Forest Food Resources, College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, 311300, China., Wang Y; National Key Laboratory for Development and Utilization of Forest Food Resources, College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, 311300, China.; College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China. |
| Πηγή: | The New phytologist [New Phytol] 2026 Sep; Vol. 251 (5), pp. 2637-2655. Date of Electronic Publication: 2026 Jun 29. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Oxford : Wiley on behalf of New Phytologist Trust Original Publication: London, New York [etc.] Academic Press. |
| Ιατρικοί όροι (MeSH): | Betula*/genetics , Betula*/physiology , Drought Resistance*/genetics , Reactive Oxygen Species*/metabolism , Transcription Factors*/metabolism , Transcription Factors*/genetics , Plant Proteins*/metabolism , Plant Proteins*/genetics , RNA, Long Noncoding*/genetics , RNA, Long Noncoding*/metabolism , Free Radical Scavengers*/metabolism , Adaptation, Physiological*/genetics, Plant Roots/growth & development ; Plant Roots/genetics ; Promoter Regions, Genetic/genetics ; Stress, Physiological/genetics ; Gene Expression Regulation, Plant ; Droughts ; Abscisic Acid ; Base Sequence |
| Περίληψη: | Drought severely limits tree growth. While long noncoding RNAs (lncRNAs) are crucial to plant stress responses, their mechanisms in drought adaptation remain unclear. Using RNA-seq, chromatin immunoprecipitation, yeast one-hybrid, electrophoretic mobility shift assay, chromatin isolation by RNA purification, and dual-luciferase reporter assays, we investigated the molecular mechanism by which the drought-inducible lncRNA BplncW20 regulates drought responses in Betula platyphylla (birch). Under normal conditions, BplncW20 overexpression (OE) promoted root growth, increased the root-to-shoot ratio, and reduced plant height without affecting biomass; under drought stress, it enhanced root development, biomass, root-to-shoot ratio, and drought tolerance, whereas the bplncw20# mutants showed opposite phenotypes. BplncW20 significantly activated key genes involved in water deprivation and hydrogen peroxide response pathways. It upregulated BpFSD2 expression by binding to its promoter, thereby enhancing antioxidant capacity and reducing the accumulation of reactive oxygen species and malondialdehyde. The transcription factor BpMYB73 acted as an upstream regulator of BplncW20, binding to MYB Binding Site (MBS) and abscisic acid-responsive element cis-elements in the BplncW20 promoter to initiate its transcription. Overexpressing BpMYB73 enhances root development and drought tolerance, similar to BplncW20 OE plants. This study proposed a putative regulatory pathway of BpMYB73-MBS/ABRE-BplncW20-target genes, revealing the critical role of BplncW20 in root development and drought adaptation. (© 2026 The Author(s). New Phytologist © 2026 New Phytologist Foundation.) |
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| Grant Information: | 32471896 National Natural Science Foundation of China; 32501673 National Natural Science Foundation of China; KJRC2023D02 Innovational Fund for Scientific and Technological Personnel of Hainan Province |
| Contributed Indexing: | Keywords: Betula platyphylla; BpMYB73; BplncW20; drought tolerance; long noncoding RNA; reactive oxygen species (ROS) scavenging |
| Substance Nomenclature: | 0 (Reactive Oxygen Species) 0 (Transcription Factors) 0 (Plant Proteins) 0 (RNA, Long Noncoding) 0 (Free Radical Scavengers) 72S9A8J5GW (Abscisic Acid) |
| SCR Organism: | Betula platyphylla |
| Entry Date(s): | Date Created: 20260630 Date Completed: 20260806 Latest Revision: 20260813 |
| Update Code: | 20260814 |
| PubMed Central ID: | PMC13443011 |
| DOI: | 10.1111/nph.71391 |
| PMID: | 42374588 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1469-8137 |
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| DOI: | 10.1111/nph.71391 |