Academic Journal
OsOSCA2.4 regulates post-Golgi trafficking of storage proteins by modulating Ca2+ homeostasis in rice endosperm.
| Τίτλος: | OsOSCA2.4 regulates post-Golgi trafficking of storage proteins by modulating Ca2+ homeostasis in rice endosperm. |
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| Συγγραφείς: | Zhang Y; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Zhu Y; State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Zhang P; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Tian W; State Key Laboratory of Protein and Plant Gene Research, School of Advanced Agricultural Sciences, Peking University, Beijing 100871, China., Dong H; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Liu Z; College of Life Science, Capital Normal University, Beijing 100048, China., Chen Y; State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Bao X; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Pan T; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Xiong Y; State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Wang X; State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Ren Z; State Key Laboratory of Protein and Plant Gene Research, School of Advanced Agricultural Sciences, Peking University, Beijing 100871, China., Jiang X; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Han X; State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Jing R; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Wu H; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Gu C; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Chen R; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Chen X; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Lei J; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Duan E; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Lei C; State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Cheng Z; State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Wang J; State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Bao Y; College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China., Wu C; State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Wang H; State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Wang Y; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China., Ren Y; State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Wan J; State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Nanjing Agricultural University, Nanjing 210095, China.; State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. |
| Πηγή: | The Plant cell [Plant Cell] 2026 Jul 02; Vol. 38 (7). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9208688 Publication Model: Print Cited Medium: Internet ISSN: 1532-298X (Electronic) Linking ISSN: 10404651 NLM ISO Abbreviation: Plant Cell Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2021- : [Oxford] : Oxford University Press Original Publication: Rockville, MD : American Society of Plant Physiologists, c1989- |
| Ιατρικοί όροι (MeSH): | Oryza*/metabolism , Oryza*/genetics , Endosperm*/metabolism , Endosperm*/genetics , Calcium*/metabolism , Golgi Apparatus*/metabolism , Plant Proteins*/metabolism , Plant Proteins*/genetics , Seed Storage Proteins*/metabolism , Seed Storage Proteins*/genetics, Glutens/metabolism ; trans-Golgi Network/metabolism ; Homeostasis ; Protein Transport ; Mutation ; Plants, Genetically Modified ; Gene Expression Regulation, Plant |
| Περίληψη: | Seed storage proteins (SSPs), which accumulate specifically during seed development, constitute a major source of plant-derived protein in the human diet. Despite their critical role in determining crop quality, the molecular mechanisms underlying the intracellular trafficking of SSPs remain poorly understood. Here, we characterize the rice glutelin precursor accumulation16 (gpa16) mutant, which exhibits defective dense vesicle (DV)-mediated post-Golgi trafficking of proglutelins, resulting in their overaccumulation in the apoplast. GPA16 encodes OsOSCA2.4, a member of the reduced hyperosmolality-induced Ca2+ increase channel (OSCA) family. OsOSCA2.4 localizes to post-Golgi compartments including the trans-Golgi network (TGN) and prevacuolar compartment (PVC) in vegetative tissue, and DVs in the endosperm. The OsOSCA2.4(P397L) variant disrupts the formation of the Rab5a molecular module, a process influenced by intracellular Ca2+ homeostasis. Ca2+ imaging assays indicate that OsOSCA2.4 functions as a regulator of Ca2+ homeostasis of PVCs/DVs, the primary sites of Rab5a module activity. Genetic analyses reveal functional redundancy between OsOSCA2.4 and OsOSCA4.1 in modulating post-Golgi trafficking of proglutelins. Collectively, our findings identify OSCA-mediated Ca2+ homeostasis as a previously unrecognized regulatory layer governing post-Golgi trafficking of SSPs and uncover an unexpected role for OSCA proteins in vesicular trafficking in eukaryotes. (© The Author(s) 2026. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.) |
| Competing Interests: | Conflicts of interest: The authors declare no competing interests. |
| Grant Information: | 2021YFF1000200 National Key Research and Development Program of China; 31830064 National Natural Science Foundation of China; 32172085 National Natural Science Foundation of China; CAAS-CSNCB-202302 Basic Research Center, Innovation Program of Chinese Academy of Agricultural Sciences; S2024ZD01 Central Public-interest Scientific Institution Basal Research Fund; CARS-01-05 China Agriculture Research System |
| Substance Nomenclature: | SY7Q814VUP (Calcium) 0 (Plant Proteins) 8002-80-0 (Glutens) 0 (Seed Storage Proteins) |
| Entry Date(s): | Date Created: 20260627 Date Completed: 20260729 Latest Revision: 20260730 |
| Update Code: | 20260730 |
| DOI: | 10.1093/plcell/koag193 |
| PMID: | 42364252 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1532-298X |
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| DOI: | 10.1093/plcell/koag193 |