Academic Journal
Προβολή σε EDS

Proteomic analysis of extracellular vesicles released from endothelial cells in vitro reveals increased levels of E-selectin and dual specificity phosphatase 7 as a potential marker of TNFα-mediated apoptosis.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Proteomic analysis of extracellular vesicles released from endothelial cells in vitro reveals increased levels of E-selectin and dual specificity phosphatase 7 as a potential marker of TNFα-mediated apoptosis.
Συγγραφείς: Collier MEW; Department of Cardiovascular Sciences, College of Life Sciences, University of Leicester, Leicester, United Kingdom.; National Institute for Health and Care Research Leicester Biomedical Research Centre, University Hospitals of Leicester NHS Trust, Glenfield Hospital, Leicester, United Kingdom., Cao TH; Department of Cardiovascular Sciences, College of Life Sciences, University of Leicester, Leicester, United Kingdom.; National Institute for Health and Care Research Leicester Biomedical Research Centre, University Hospitals of Leicester NHS Trust, Glenfield Hospital, Leicester, United Kingdom.; Leicester van Geest Multi-OMICS facility, University of Leicester, Leicester, United Kingdom., Quinn PA; Department of Cardiovascular Sciences, College of Life Sciences, University of Leicester, Leicester, United Kingdom.; Leicester van Geest Multi-OMICS facility, University of Leicester, Leicester, United Kingdom., Sandhu JK; Department of Cardiovascular Sciences, College of Life Sciences, University of Leicester, Leicester, United Kingdom.; Leicester van Geest Multi-OMICS facility, University of Leicester, Leicester, United Kingdom., Jones DJL; Department of Cardiovascular Sciences, College of Life Sciences, University of Leicester, Leicester, United Kingdom.; National Institute for Health and Care Research Leicester Biomedical Research Centre, University Hospitals of Leicester NHS Trust, Glenfield Hospital, Leicester, United Kingdom.; Leicester van Geest Multi-OMICS facility, University of Leicester, Leicester, United Kingdom.; Leicester Cancer Research Centre, University Hospitals of Leicester NHS Trust, Leicester Royal Infirmary, University of Leicester, Leicester, United Kingdom., Goodall AH; Department of Cardiovascular Sciences, College of Life Sciences, University of Leicester, Leicester, United Kingdom.; National Institute for Health and Care Research Leicester Biomedical Research Centre, University Hospitals of Leicester NHS Trust, Glenfield Hospital, Leicester, United Kingdom.
Πηγή: PloS one [PLoS One] 2026 Jun 26; Vol. 21 (6), pp. e0352394. Date of Electronic Publication: 2026 Jun 26 (Print Publication: 2026).
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
Imprint Name(s): Original Publication: San Francisco, CA : Public Library of Science
Ιατρικοί όροι (MeSH): Tumor Necrosis Factor-alpha*/pharmacology , Tumor Necrosis Factor-alpha*/metabolism , Apoptosis*/drug effects , Extracellular Vesicles*/metabolism , Extracellular Vesicles*/drug effects , Human Umbilical Vein Endothelial Cells*/metabolism , Human Umbilical Vein Endothelial Cells*/drug effects , E-Selectin*/metabolism , Proteomics*/methods , Protein Tyrosine Phosphatases*/metabolism, Biomarkers/metabolism ; Lipoproteins, LDL/pharmacology ; Proteome/metabolism ; Endothelial Cells/metabolism ; Caspase 7/metabolism ; Humans ; Tandem Mass Spectrometry
Περίληψη: Background: Proteins can be actively packaged into extracellular vesicles (EVs) through mechanisms dependent on the stimulus that activated the cells. Identifying proteins released in endothelial EVs in response to stimuli relevant to cardiovascular disease (CVD) may therefore reveal potential biomarkers that provide information about the vascular endothelium. This study aimed to identify differentially expressed proteins in EVs released from human umbilical vein endothelial cells (HUVEC) in response to stimuli relevant to vascular endothelium activation.
Methods and Results: HUVEC were stimulated with tumour necrosis factor alpha (TNFα) (10 ng/mL) or oxidised low-density lipoprotein (oxLDL) (10 µg/mL). Apoptosis was assessed using a flow cytometric DNA fragmentation protocol and caspase-3/7 activity assay. Size distributions of EVs were examined by nanoparticle tracking analysis. Isolated EVs were examined using tandem liquid-chromatography-mass spectrometry (LC-MS/MS). While treatment of HUVEC with TNFα or oxLDL resulted in non-significant elevations in levels of EVs, only TNFα increased apoptosis. Mass spectrometry quantified 1355 proteins and revealed significant differences in the proteome of EVs from TNFα-treated HUVEC compared to EVs from oxLDL-treated or untreated cells. Several candidate biomarkers were significantly and differentially expressed in response to TNFα, including E-selectin and dual specificity phosphatase 7.
Conclusions: This study further associated E-selectin on endothelial-derived EVs with endothelial apoptosis and may offer a biomarker of endothelial damage in patients with CVD.
(Copyright: © 2026 Collier et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Competing Interests: The authors have declared that no competing interests exist.
References: J Hum Hypertens. 2009 May;23(5):307-15. (PMID: 19037229)
Am J Physiol. 1998 Aug;275(2):H568-76. (PMID: 9683446)
J Extracell Vesicles. 2012 Apr 16;1:. (PMID: 24009886)
Cell Tissue Res. 2022 Mar;387(3):391-398. (PMID: 34014399)
J Thromb Haemost. 2009 Apr;7(4):701-9. (PMID: 19192109)
Mol Med Rep. 2020 Oct;22(4):2981-2989. (PMID: 32945438)
J Extracell Vesicles. 2024 Feb;13(2):e12404. (PMID: 38326288)
Nat Commun. 2013;4:2980. (PMID: 24356509)
Am Heart J. 2003 Jun;145(6):962-70. (PMID: 12796750)
Am J Physiol Cell Physiol. 2005 Nov;289(5):C1229-39. (PMID: 15972838)
Arterioscler Thromb Vasc Biol. 2001 May;21(5):844-8. (PMID: 11348884)
Thromb Res. 2003 Feb 15;109(4):175-80. (PMID: 12757771)
Biochem Biophys Res Commun. 2017 Jan 29;483(1):45-51. (PMID: 28063928)
J Thromb Haemost. 2003 Nov;1(11):2434-43. (PMID: 14629480)
Diabetologia. 1992 Aug;35(8):771-6. (PMID: 1511805)
Am J Physiol Cell Physiol. 2017 Nov 1;313(5):C567-C574. (PMID: 28814403)
PLoS One. 2013 Dec 02;8(12):e81930. (PMID: 24349153)
J Extracell Vesicles. 2017 Apr 19;6(1):1302705. (PMID: 28473883)
Int J Cardiol. 2014 Jul 15;175(1):203. (PMID: 24820735)
Kidney Int. 1994 Sep;46(3):807-13. (PMID: 7996802)
Nucleic Acids Res. 2015 Apr 20;43(7):e47. (PMID: 25605792)
Cell. 2019 Apr 4;177(2):428-445.e18. (PMID: 30951670)
Shock. 2008 Apr;29(4):504-11. (PMID: 18598005)
Oncogene. 2003 Oct 23;22(48):7649-60. (PMID: 14576828)
Circulation. 2000 May 9;101(18):2149-53. (PMID: 10801754)
Clin Sci (Lond). 2006 Mar;110(3):353-60. (PMID: 16309383)
Arterioscler Thromb Vasc Biol. 2003 Apr 1;23(4):695-701. (PMID: 12692009)
Mol Med Rep. 2013 Jan;7(1):318-26. (PMID: 23124128)
J Extracell Vesicles. 2020 Aug 11;9(1):1801153. (PMID: 32944190)
Circ Res. 2013 May 10;112(10):1323-33. (PMID: 23536307)
Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E968-77. (PMID: 26858453)
Proteomics. 2008 Jun;8(12):2430-46. (PMID: 18563738)
Nature. 1980 Nov 27;288(5789):373-6. (PMID: 6253831)
Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613. (PMID: 30476243)
J Heart Lung Transplant. 2009 Oct;28(10):1081-6. (PMID: 19782291)
J Biol Chem. 1999 Nov 5;274(45):32512-9. (PMID: 10542298)
Biochem Biophys Res Commun. 2000 Jun 16;272(3):872-6. (PMID: 10860844)
J Immunol. 1996 Apr 1;156(7):2558-65. (PMID: 8786319)
J Am Coll Cardiol. 2009 Aug 11;54(7):601-8. (PMID: 19660689)
Nat Methods. 2014 Feb;11(2):167-70. (PMID: 24336358)
Proteomics. 2005 Nov;5(17):4443-55. (PMID: 16220532)
Med J Islam Repub Iran. 2016 Jul 16;30:398. (PMID: 27579288)
Biochem J. 1995 Feb 15;306 ( Pt 1):293-8. (PMID: 7532399)
Biochem Biophys Res Commun. 2014 May 23;448(1):101-7. (PMID: 24769202)
Angiology. 1993 Apr;44(4):332-7. (PMID: 8457086)
Arterioscler Thromb Vasc Biol. 2003 May 1;23(5):816-21. (PMID: 12637341)
J Biol Chem. 1998 Apr 17;273(16):9681-7. (PMID: 9545302)
Circ Res. 2016 Feb 19;118(4):620-36. (PMID: 26892962)
Arterioscler Thromb Vasc Biol. 2006 Jan;26(1):112-6. (PMID: 16239600)
J Pathol. 1993 Nov;171(3):223-9. (PMID: 7506307)
N Engl J Med. 1986 Oct 23;315(17):1046-51. (PMID: 3093861)
Circ Res. 2001 May 11;88(9):877-87. (PMID: 11348996)
Substance Nomenclature: 0 (Tumor Necrosis Factor-alpha)
0 (E-Selectin)
0 (Biomarkers)
0 (oxidized low density lipoprotein)
0 (Lipoproteins, LDL)
EC 3.1.3.48 (Protein Tyrosine Phosphatases)
0 (Proteome)
EC 3.4.22.- (Caspase 7)
Entry Date(s): Date Created: 20260626 Date Completed: 20260626 Latest Revision: 20260813
Update Code: 20260813
PubMed Central ID: PMC13308812
DOI: 10.1371/journal.pone.0352394
PMID: 42361017
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1932-6203
DOI:10.1371/journal.pone.0352394