Academic Journal
Προβολή σε EDS

IgM and IgG Epitope Mapping of the Porin Outer Membrane Protein-2a from Brucella abortus: Potential Biomarkers for Detecting Exposure to Brucellosis.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: IgM and IgG Epitope Mapping of the Porin Outer Membrane Protein-2a from Brucella abortus: Potential Biomarkers for Detecting Exposure to Brucellosis.
Συγγραφείς: Noguera AF; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil., Lechuga GC; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil., Napoleão-Pêgo P; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil., Carvalho JPRS; Program of Post-Graduation on Science and Biotechnology, Department of Molecular and Cellular Biology, Biology Institute, Federal Fluminense University, Niterói 22040-036, Brazil., Gomes LR; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil., Carneiro da Silva A; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil., Melo Monnerat M; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil., da Silva FR; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil., De-Simone SG; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil.; Program of Post-Graduation on Science and Biotechnology, Department of Molecular and Cellular Biology, Biology Institute, Federal Fluminense University, Niterói 22040-036, Brazil.; Program of Post-Graduation on Parasitic Biology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-900, Brazil.
Πηγή: International journal of molecular sciences [Int J Mol Sci] 2026 Jun 13; Vol. 27 (12). Date of Electronic Publication: 2026 Jun 13.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
Imprint Name(s): Original Publication: Basel, Switzerland : MDPI, [2000-
Ιατρικοί όροι (MeSH): Immunoglobulin M*/immunology , Immunoglobulin G*/immunology , Epitope Mapping*/methods , Brucella abortus*/immunology , Brucellosis*/diagnosis , Brucellosis*/immunology , Brucellosis*/microbiology , Brucellosis*/blood , Porins*/immunology , Bacterial Outer Membrane Proteins*/immunology, Biomarkers/blood ; Antibodies, Bacterial/immunology ; Epitopes, B-Lymphocyte/immunology ; Antigens, Bacterial/immunology ; Humans ; Enzyme-Linked Immunosorbent Assay ; Animals ; Cross Reactions
Περίληψη: Brucellosis is a globally prevalent zoonotic disease affecting both humans and animals. Its nonspecific clinical manifestations often complicate diagnosis, underscoring the need for reliable laboratory confirmation. Traditional serological assays, though widely used, suffer from limitations such as inconsistent sensitivity and false-positive results. To address these challenges, this study mapped IgM and IgG epitopes of the Brucella Omp-2a protein using sera from infected patients. Epitope identification was performed through SPOT synthesis on cellulose membranes, followed by assessment of potential cross-reactivity using peptide database analysis and ELISA validation. Three major IgM and seven IgG linear B-cell epitopes were identified, six of which demonstrated strong reactivity in peptide-ELISA. Importantly, no significant cross-reactivity with proteins from other human pathogens was detected. Two chimeric multi-epitope peptides, composed of 50 and 60 amino acids and integrating Brucella-specific IgM and IgG epitopes, exhibited excellent diagnostic performance in ELISA, achieving near 100% sensitivity and specificity. These findings support the potential of synthetic peptides as reliable and cost-effective alternatives to native antigens in serological assays. Further validation in larger, geographically diverse cohorts will be essential to confirm their diagnostic robustness and facilitate their integration into routine brucellosis diagnostics.
References: Folia Microbiol (Praha). 2020 Apr;65(2):353-361. (PMID: 31368088)
Protein Expr Purif. 2012 Jun;83(2):198-204. (PMID: 22538317)
Zoonoses Public Health. 2009 May;56(4):183-7. (PMID: 18811674)
PLoS Negl Trop Dis. 2014 Dec 04;8(12):e3390. (PMID: 25474572)
Biomed Res Int. 2018 Apr 22;2018:5712920. (PMID: 29850535)
Infect Immun. 1993 Sep;61(9):3785-90. (PMID: 7689540)
Vaccines (Basel). 2021 Mar 26;9(4):. (PMID: 33810325)
PLoS Negl Trop Dis. 2014 Jul 24;8(7):e3008. (PMID: 25058178)
J Microbiol Methods. 2008 Oct;75(2):375-8. (PMID: 18675856)
BMC Vet Res. 2020 Feb 18;16(1):61. (PMID: 32070337)
Vet World. 2023;16(7):1390-1399. (PMID: 37621538)
PLoS Negl Trop Dis. 2024 Mar 07;18(3):e0012030. (PMID: 38452046)
Cent Eur J Immunol. 2018;43(1):81-89. (PMID: 29736150)
Vaccines (Basel). 2023 Mar 14;11(3):. (PMID: 36992237)
Vet World. 2024 Apr;17(4):811-819. (PMID: 38798281)
Emerg Microbes Infect. 2024 Dec;13(1):2290839. (PMID: 38039063)
Toxins (Basel). 2023 Mar 24;15(4):. (PMID: 37104177)
Eur J Clin Microbiol Infect Dis. 2004 Mar;23(3):194-9. (PMID: 14986157)
J Microbiol Methods. 2014 Feb;97:25-8. (PMID: 24345764)
Appl Biosaf. 2023 Jun 1;28(2):72-86. (PMID: 37342513)
Biomed Pharmacother. 2022 Nov;155:113557. (PMID: 36115112)
Front Vet Sci. 2021 Sep 08;8:708008. (PMID: 34568475)
Sultan Qaboos Univ Med J. 2021 May;21(2):e282-e288. (PMID: 34221477)
Clin Diagn Lab Immunol. 2003 Jul;10(4):710-4. (PMID: 12853409)
Ital J Food Saf. 2016 Jun 03;5(3):5913. (PMID: 27853716)
Am J Transl Res. 2024 Aug 15;16(8):3915-3921. (PMID: 39262700)
Epidemiol Infect. 1992 Feb;108(1):77-85. (PMID: 1547843)
Infect Disord Drug Targets. 2019;19(1):36-45. (PMID: 29984663)
Ann Med. 2023;55(2):2295398. (PMID: 38165919)
Prev Vet Med. 2023 Apr;213:105878. (PMID: 36857972)
J Infect Public Health. 2019 May - Jun;12(3):337-342. (PMID: 30553722)
Pathogens. 2021 Dec 14;10(12):. (PMID: 34959578)
J Biomol Struct Dyn. 2000 Feb;17(4):747-57. (PMID: 10698111)
Lancet Infect Dis. 2014 Jun;14(6):520-6. (PMID: 24480149)
Nature. 2021 Aug;596(7873):583-589. (PMID: 34265844)
Microb Cell Fact. 2024 May 22;23(1):145. (PMID: 38778337)
Comp Immunol Microbiol Infect Dis. 2019 Aug;65:29-36. (PMID: 31300122)
Int J Gen Med. 2021 Nov 16;14:8311-8316. (PMID: 34815703)
Diagn Microbiol Infect Dis. 2009 Oct;65(2):99-102. (PMID: 19748418)
Mol Immunol. 2013 Jan;53(1-2):24-34. (PMID: 22784991)
FEMS Microbiol Lett. 1997 Oct 1;155(1):23-30. (PMID: 9345760)
PLoS One. 2017 Mar 16;12(3):e0173365. (PMID: 28301498)
Vaccine. 2014 Oct 29;32(47):6251-8. (PMID: 25252193)
ScientificWorldJournal. 2012;2012:104239. (PMID: 22566755)
Sci Rep. 2021 Jul 6;11(1):13943. (PMID: 34230531)
PLoS Negl Trop Dis. 2019 Jun 19;13(6):e0007509. (PMID: 31216271)
China CDC Wkly. 2021 Feb 5;3(6):120-123. (PMID: 34595017)
Emerg Infect Dis. 2023 Sep;29(9):1789-1797. (PMID: 37610167)
Front Immunol. 2021 Dec 21;12:778475. (PMID: 34992597)
Foodborne Pathog Dis. 2012 Mar;9(3):190-7. (PMID: 22300225)
Indian J Med Microbiol. 2007 Jul;25(3):188-202. (PMID: 17901634)
J Infect Public Health. 2012 Apr;5(2):189-94. (PMID: 22541267)
Clin Vaccine Immunol. 2012 Aug;19(8):1332-4. (PMID: 22718127)
Vet Res Forum. 2022 Sep;13(3):387-391. (PMID: 36320311)
Int J Med Sci. 2011;8(5):428-32. (PMID: 21814476)
BMC Infect Dis. 2017 Jul 11;17(1):485. (PMID: 28693438)
Lancet Infect Dis. 2007 Dec;7(12):775-86. (PMID: 18045560)
Sao Paulo Med J. 2020 Nov-Dec;138(6):561-562. (PMID: 33206914)
Transbound Emerg Dis. 2022 Sep;69(5):e1253-e1268. (PMID: 35244335)
Int J Environ Res Public Health. 2019 Oct 23;16(21):. (PMID: 31652718)
Clin Diagn Lab Immunol. 2005 Nov;12(11):1269-74. (PMID: 16275939)
BMC Infect Dis. 2016 May 21;16:219. (PMID: 27206475)
J Microbiol Methods. 2020 Jul;174:105964. (PMID: 32479870)
Vet World. 2020 Jul;13(7):1439-1447. (PMID: 32848322)
J Bacteriol. 2001 Aug;183(16):4839-47. (PMID: 11466287)
Rev Chilena Infectol. 2008 Apr;25(2):116-21. (PMID: 18483644)
Epidemiol Infect. 2021 May 25;149:e136. (PMID: 34032200)
Microb Pathog. 2020 Oct;147:104318. (PMID: 32531499)
Toxicon. 2017 Nov;138:37-42. (PMID: 28811248)
Vet World. 2021 Aug;14(8):2187-2196. (PMID: 34566338)
PLoS Negl Trop Dis. 2021 Aug 17;15(8):e0009695. (PMID: 34403421)
Grant Information: #301744/2019-0 Brazilian Council for Scientific Research (CNPq)
Contributed Indexing: Keywords: Brucella abortus infection; ELISA-peptide; IgM and IgG epitope mapping; Omp-2a; brucellosis; diagnostic; transmembrane protein
Substance Nomenclature: 0 (Immunoglobulin M)
0 (Immunoglobulin G)
0 (Porins)
0 (Bacterial Outer Membrane Proteins)
0 (Biomarkers)
0 (Antibodies, Bacterial)
0 (Epitopes, B-Lymphocyte)
0 (Antigens, Bacterial)
Entry Date(s): Date Created: 20260626 Date Completed: 20260626 Latest Revision: 20260813
Update Code: 20260814
PubMed Central ID: PMC13299733
DOI: 10.3390/ijms27125341
PMID: 42353060
Βάση Δεδομένων: MEDLINE
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://resolver.ebsco.com/c/fiv2js/result?sid=EBSCO:cmedm&genre=article&issn=14220067&ISBN=&volume=27&issue=12&date=20260613&spage=&pages=&title=International journal of molecular sciences&atitle=IgM%20and%20IgG%20Epitope%20Mapping%20of%20the%20Porin%20Outer%20Membrane%20Protein-2a%20from%20Brucella%20abortus%3A%20Potential%20Biomarkers%20for%20Detecting%20Exposure%20to%20Brucellosis.&aulast=Noguera%20AF&id=DOI:10.3390/ijms27125341
    Name: Full Text Finder (for New FTF UI) (ns324271)
    Category: fullText
    Text: Full Text Finder
    MouseOverText: Full Text Finder
Header DbId: cmedm
DbLabel: MEDLINE
An: 42353060
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: IgM and IgG Epitope Mapping of the Porin Outer Membrane Protein-2a from Brucella abortus: Potential Biomarkers for Detecting Exposure to Brucellosis.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Noguera+AF%22">Noguera AF</searchLink>; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil.<br /><searchLink fieldCode="AU" term="%22Lechuga+GC%22">Lechuga GC</searchLink>; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.<br /><searchLink fieldCode="AU" term="%22Napoleão-Pêgo+P%22">Napoleão-Pêgo P</searchLink>; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil.<br /><searchLink fieldCode="AU" term="%22Carvalho+JPRS%22">Carvalho JPRS</searchLink>; Program of Post-Graduation on Science and Biotechnology, Department of Molecular and Cellular Biology, Biology Institute, Federal Fluminense University, Niterói 22040-036, Brazil.<br /><searchLink fieldCode="AU" term="%22Gomes+LR%22">Gomes LR</searchLink>; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.<br /><searchLink fieldCode="AU" term="%22Carneiro+da+Silva+A%22">Carneiro da Silva A</searchLink>; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil.<br /><searchLink fieldCode="AU" term="%22Melo+Monnerat+M%22">Melo Monnerat M</searchLink>; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil.<br /><searchLink fieldCode="AU" term="%22da+Silva+FR%22">da Silva FR</searchLink>; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.<br /><searchLink fieldCode="AU" term="%22De-Simone+SG%22">De-Simone SG</searchLink>; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil.; Program of Post-Graduation on Science and Biotechnology, Department of Molecular and Cellular Biology, Biology Institute, Federal Fluminense University, Niterói 22040-036, Brazil.; Program of Post-Graduation on Parasitic Biology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-900, Brazil.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22101092791%22">International journal of molecular sciences</searchLink> [Int J Mol Sci] 2026 Jun 13; Vol. 27 (12). <i>Date of Electronic Publication: </i>2026 Jun 13.
– Name: TypePub
  Label: Publication Type
  Group: TypPub
  Data: Journal Article
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22MDPI%22">MDPI </searchLink><i>Country of Publication: </i>Switzerland <i>NLM ID: </i>101092791 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1422-0067 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2214220067%22">14220067 </searchLink><i>NLM ISO Abbreviation: </i>Int J Mol Sci <i>Subsets: </i>MEDLINE
– Name: PublisherInfo
  Label: Imprint Name(s)
  Group: PubInfo
  Data: <i>Original Publication</i>: Basel, Switzerland : MDPI, [2000-
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Immunoglobulin+M%22">Immunoglobulin M*</searchLink>/<searchLink fieldCode="MM" term="%22Immunoglobulin+M+immunology%22">immunology</searchLink> <br /><searchLink fieldCode="MM" term="%22Immunoglobulin+G%22">Immunoglobulin G*</searchLink>/<searchLink fieldCode="MM" term="%22Immunoglobulin+G+immunology%22">immunology</searchLink> <br /><searchLink fieldCode="MM" term="%22Epitope+Mapping%22">Epitope Mapping*</searchLink>/<searchLink fieldCode="MM" term="%22Epitope+Mapping+methods%22">methods</searchLink> <br /><searchLink fieldCode="MM" term="%22Brucella+abortus%22">Brucella abortus*</searchLink>/<searchLink fieldCode="MM" term="%22Brucella+abortus+immunology%22">immunology</searchLink> <br /><searchLink fieldCode="MM" term="%22Brucellosis%22">Brucellosis*</searchLink>/<searchLink fieldCode="MM" term="%22Brucellosis+diagnosis%22">diagnosis</searchLink> <br /><searchLink fieldCode="MM" term="%22Brucellosis%22">Brucellosis*</searchLink>/<searchLink fieldCode="MM" term="%22Brucellosis+immunology%22">immunology</searchLink> <br /><searchLink fieldCode="MM" term="%22Brucellosis%22">Brucellosis*</searchLink>/<searchLink fieldCode="MM" term="%22Brucellosis+microbiology%22">microbiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Brucellosis%22">Brucellosis*</searchLink>/<searchLink fieldCode="MM" term="%22Brucellosis+blood%22">blood</searchLink> <br /><searchLink fieldCode="MM" term="%22Porins%22">Porins*</searchLink>/<searchLink fieldCode="MM" term="%22Porins+immunology%22">immunology</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacterial+Outer+Membrane+Proteins%22">Bacterial Outer Membrane Proteins*</searchLink>/<searchLink fieldCode="MM" term="%22Bacterial+Outer+Membrane+Proteins+immunology%22">immunology</searchLink><br /><searchLink fieldCode="MH" term="%22Biomarkers%22">Biomarkers</searchLink>/<searchLink fieldCode="MH" term="%22Biomarkers+blood%22">blood</searchLink> ; <searchLink fieldCode="MH" term="%22Antibodies%2C+Bacterial%22">Antibodies, Bacterial</searchLink>/<searchLink fieldCode="MH" term="%22Antibodies%2C+Bacterial+immunology%22">immunology</searchLink> ; <searchLink fieldCode="MH" term="%22Epitopes%2C+B-Lymphocyte%22">Epitopes, B-Lymphocyte</searchLink>/<searchLink fieldCode="MH" term="%22Epitopes%2C+B-Lymphocyte+immunology%22">immunology</searchLink> ; <searchLink fieldCode="MH" term="%22Antigens%2C+Bacterial%22">Antigens, Bacterial</searchLink>/<searchLink fieldCode="MH" term="%22Antigens%2C+Bacterial+immunology%22">immunology</searchLink> ; <searchLink fieldCode="MH" term="%22Humans%22">Humans</searchLink> ; <searchLink fieldCode="MH" term="%22Enzyme-Linked+Immunosorbent+Assay%22">Enzyme-Linked Immunosorbent Assay</searchLink> ; <searchLink fieldCode="MH" term="%22Animals%22">Animals</searchLink> ; <searchLink fieldCode="MH" term="%22Cross+Reactions%22">Cross Reactions</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Brucellosis is a globally prevalent zoonotic disease affecting both humans and animals. Its nonspecific clinical manifestations often complicate diagnosis, underscoring the need for reliable laboratory confirmation. Traditional serological assays, though widely used, suffer from limitations such as inconsistent sensitivity and false-positive results. To address these challenges, this study mapped IgM and IgG epitopes of the Brucella Omp-2a protein using sera from infected patients. Epitope identification was performed through SPOT synthesis on cellulose membranes, followed by assessment of potential cross-reactivity using peptide database analysis and ELISA validation. Three major IgM and seven IgG linear B-cell epitopes were identified, six of which demonstrated strong reactivity in peptide-ELISA. Importantly, no significant cross-reactivity with proteins from other human pathogens was detected. Two chimeric multi-epitope peptides, composed of 50 and 60 amino acids and integrating Brucella-specific IgM and IgG epitopes, exhibited excellent diagnostic performance in ELISA, achieving near 100% sensitivity and specificity. These findings support the potential of synthetic peptides as reliable and cost-effective alternatives to native antigens in serological assays. Further validation in larger, geographically diverse cohorts will be essential to confirm their diagnostic robustness and facilitate their integration into routine brucellosis diagnostics.
– Name: Ref
  Label: References
  Group: RefInfo
  Data: Folia Microbiol (Praha). 2020 Apr;65(2):353-361. (PMID: <searchLink fieldCode="PM" term="%2231368088%22">31368088)</searchLink><br />Protein Expr Purif. 2012 Jun;83(2):198-204. (PMID: <searchLink fieldCode="PM" term="%2222538317%22">22538317)</searchLink><br />Zoonoses Public Health. 2009 May;56(4):183-7. (PMID: <searchLink fieldCode="PM" term="%2218811674%22">18811674)</searchLink><br />PLoS Negl Trop Dis. 2014 Dec 04;8(12):e3390. (PMID: <searchLink fieldCode="PM" term="%2225474572%22">25474572)</searchLink><br />Biomed Res Int. 2018 Apr 22;2018:5712920. (PMID: <searchLink fieldCode="PM" term="%2229850535%22">29850535)</searchLink><br />Infect Immun. 1993 Sep;61(9):3785-90. (PMID: <searchLink fieldCode="PM" term="%227689540%22">7689540)</searchLink><br />Vaccines (Basel). 2021 Mar 26;9(4):. (PMID: <searchLink fieldCode="PM" term="%2233810325%22">33810325)</searchLink><br />PLoS Negl Trop Dis. 2014 Jul 24;8(7):e3008. (PMID: <searchLink fieldCode="PM" term="%2225058178%22">25058178)</searchLink><br />J Microbiol Methods. 2008 Oct;75(2):375-8. (PMID: <searchLink fieldCode="PM" term="%2218675856%22">18675856)</searchLink><br />BMC Vet Res. 2020 Feb 18;16(1):61. (PMID: <searchLink fieldCode="PM" term="%2232070337%22">32070337)</searchLink><br />Vet World. 2023;16(7):1390-1399. (PMID: <searchLink fieldCode="PM" term="%2237621538%22">37621538)</searchLink><br />PLoS Negl Trop Dis. 2024 Mar 07;18(3):e0012030. (PMID: <searchLink fieldCode="PM" term="%2238452046%22">38452046)</searchLink><br />Cent Eur J Immunol. 2018;43(1):81-89. (PMID: <searchLink fieldCode="PM" term="%2229736150%22">29736150)</searchLink><br />Vaccines (Basel). 2023 Mar 14;11(3):. (PMID: <searchLink fieldCode="PM" term="%2236992237%22">36992237)</searchLink><br />Vet World. 2024 Apr;17(4):811-819. (PMID: <searchLink fieldCode="PM" term="%2238798281%22">38798281)</searchLink><br />Emerg Microbes Infect. 2024 Dec;13(1):2290839. (PMID: <searchLink fieldCode="PM" term="%2238039063%22">38039063)</searchLink><br />Toxins (Basel). 2023 Mar 24;15(4):. (PMID: <searchLink fieldCode="PM" term="%2237104177%22">37104177)</searchLink><br />Eur J Clin Microbiol Infect Dis. 2004 Mar;23(3):194-9. (PMID: <searchLink fieldCode="PM" term="%2214986157%22">14986157)</searchLink><br />J Microbiol Methods. 2014 Feb;97:25-8. (PMID: <searchLink fieldCode="PM" term="%2224345764%22">24345764)</searchLink><br />Appl Biosaf. 2023 Jun 1;28(2):72-86. (PMID: <searchLink fieldCode="PM" term="%2237342513%22">37342513)</searchLink><br />Biomed Pharmacother. 2022 Nov;155:113557. (PMID: <searchLink fieldCode="PM" term="%2236115112%22">36115112)</searchLink><br />Front Vet Sci. 2021 Sep 08;8:708008. (PMID: <searchLink fieldCode="PM" term="%2234568475%22">34568475)</searchLink><br />Sultan Qaboos Univ Med J. 2021 May;21(2):e282-e288. (PMID: <searchLink fieldCode="PM" term="%2234221477%22">34221477)</searchLink><br />Clin Diagn Lab Immunol. 2003 Jul;10(4):710-4. (PMID: <searchLink fieldCode="PM" term="%2212853409%22">12853409)</searchLink><br />Ital J Food Saf. 2016 Jun 03;5(3):5913. (PMID: <searchLink fieldCode="PM" term="%2227853716%22">27853716)</searchLink><br />Am J Transl Res. 2024 Aug 15;16(8):3915-3921. (PMID: <searchLink fieldCode="PM" term="%2239262700%22">39262700)</searchLink><br />Epidemiol Infect. 1992 Feb;108(1):77-85. (PMID: <searchLink fieldCode="PM" term="%221547843%22">1547843)</searchLink><br />Infect Disord Drug Targets. 2019;19(1):36-45. (PMID: <searchLink fieldCode="PM" term="%2229984663%22">29984663)</searchLink><br />Ann Med. 2023;55(2):2295398. (PMID: <searchLink fieldCode="PM" term="%2238165919%22">38165919)</searchLink><br />Prev Vet Med. 2023 Apr;213:105878. (PMID: <searchLink fieldCode="PM" term="%2236857972%22">36857972)</searchLink><br />J Infect Public Health. 2019 May - Jun;12(3):337-342. (PMID: <searchLink fieldCode="PM" term="%2230553722%22">30553722)</searchLink><br />Pathogens. 2021 Dec 14;10(12):. (PMID: <searchLink fieldCode="PM" term="%2234959578%22">34959578)</searchLink><br />J Biomol Struct Dyn. 2000 Feb;17(4):747-57. (PMID: <searchLink fieldCode="PM" term="%2210698111%22">10698111)</searchLink><br />Lancet Infect Dis. 2014 Jun;14(6):520-6. (PMID: <searchLink fieldCode="PM" term="%2224480149%22">24480149)</searchLink><br />Nature. 2021 Aug;596(7873):583-589. (PMID: <searchLink fieldCode="PM" term="%2234265844%22">34265844)</searchLink><br />Microb Cell Fact. 2024 May 22;23(1):145. (PMID: <searchLink fieldCode="PM" term="%2238778337%22">38778337)</searchLink><br />Comp Immunol Microbiol Infect Dis. 2019 Aug;65:29-36. (PMID: <searchLink fieldCode="PM" term="%2231300122%22">31300122)</searchLink><br />Int J Gen Med. 2021 Nov 16;14:8311-8316. (PMID: <searchLink fieldCode="PM" term="%2234815703%22">34815703)</searchLink><br />Diagn Microbiol Infect Dis. 2009 Oct;65(2):99-102. (PMID: <searchLink fieldCode="PM" term="%2219748418%22">19748418)</searchLink><br />Mol Immunol. 2013 Jan;53(1-2):24-34. (PMID: <searchLink fieldCode="PM" term="%2222784991%22">22784991)</searchLink><br />FEMS Microbiol Lett. 1997 Oct 1;155(1):23-30. (PMID: <searchLink fieldCode="PM" term="%229345760%22">9345760)</searchLink><br />PLoS One. 2017 Mar 16;12(3):e0173365. (PMID: <searchLink fieldCode="PM" term="%2228301498%22">28301498)</searchLink><br />Vaccine. 2014 Oct 29;32(47):6251-8. (PMID: <searchLink fieldCode="PM" term="%2225252193%22">25252193)</searchLink><br />ScientificWorldJournal. 2012;2012:104239. (PMID: <searchLink fieldCode="PM" term="%2222566755%22">22566755)</searchLink><br />Sci Rep. 2021 Jul 6;11(1):13943. (PMID: <searchLink fieldCode="PM" term="%2234230531%22">34230531)</searchLink><br />PLoS Negl Trop Dis. 2019 Jun 19;13(6):e0007509. (PMID: <searchLink fieldCode="PM" term="%2231216271%22">31216271)</searchLink><br />China CDC Wkly. 2021 Feb 5;3(6):120-123. (PMID: <searchLink fieldCode="PM" term="%2234595017%22">34595017)</searchLink><br />Emerg Infect Dis. 2023 Sep;29(9):1789-1797. (PMID: <searchLink fieldCode="PM" term="%2237610167%22">37610167)</searchLink><br />Front Immunol. 2021 Dec 21;12:778475. (PMID: <searchLink fieldCode="PM" term="%2234992597%22">34992597)</searchLink><br />Foodborne Pathog Dis. 2012 Mar;9(3):190-7. (PMID: <searchLink fieldCode="PM" term="%2222300225%22">22300225)</searchLink><br />Indian J Med Microbiol. 2007 Jul;25(3):188-202. (PMID: <searchLink fieldCode="PM" term="%2217901634%22">17901634)</searchLink><br />J Infect Public Health. 2012 Apr;5(2):189-94. (PMID: <searchLink fieldCode="PM" term="%2222541267%22">22541267)</searchLink><br />Clin Vaccine Immunol. 2012 Aug;19(8):1332-4. (PMID: <searchLink fieldCode="PM" term="%2222718127%22">22718127)</searchLink><br />Vet Res Forum. 2022 Sep;13(3):387-391. (PMID: <searchLink fieldCode="PM" term="%2236320311%22">36320311)</searchLink><br />Int J Med Sci. 2011;8(5):428-32. (PMID: <searchLink fieldCode="PM" term="%2221814476%22">21814476)</searchLink><br />BMC Infect Dis. 2017 Jul 11;17(1):485. (PMID: <searchLink fieldCode="PM" term="%2228693438%22">28693438)</searchLink><br />Lancet Infect Dis. 2007 Dec;7(12):775-86. (PMID: <searchLink fieldCode="PM" term="%2218045560%22">18045560)</searchLink><br />Sao Paulo Med J. 2020 Nov-Dec;138(6):561-562. (PMID: <searchLink fieldCode="PM" term="%2233206914%22">33206914)</searchLink><br />Transbound Emerg Dis. 2022 Sep;69(5):e1253-e1268. (PMID: <searchLink fieldCode="PM" term="%2235244335%22">35244335)</searchLink><br />Int J Environ Res Public Health. 2019 Oct 23;16(21):. (PMID: <searchLink fieldCode="PM" term="%2231652718%22">31652718)</searchLink><br />Clin Diagn Lab Immunol. 2005 Nov;12(11):1269-74. (PMID: <searchLink fieldCode="PM" term="%2216275939%22">16275939)</searchLink><br />BMC Infect Dis. 2016 May 21;16:219. (PMID: <searchLink fieldCode="PM" term="%2227206475%22">27206475)</searchLink><br />J Microbiol Methods. 2020 Jul;174:105964. (PMID: <searchLink fieldCode="PM" term="%2232479870%22">32479870)</searchLink><br />Vet World. 2020 Jul;13(7):1439-1447. (PMID: <searchLink fieldCode="PM" term="%2232848322%22">32848322)</searchLink><br />J Bacteriol. 2001 Aug;183(16):4839-47. (PMID: <searchLink fieldCode="PM" term="%2211466287%22">11466287)</searchLink><br />Rev Chilena Infectol. 2008 Apr;25(2):116-21. (PMID: <searchLink fieldCode="PM" term="%2218483644%22">18483644)</searchLink><br />Epidemiol Infect. 2021 May 25;149:e136. (PMID: <searchLink fieldCode="PM" term="%2234032200%22">34032200)</searchLink><br />Microb Pathog. 2020 Oct;147:104318. (PMID: <searchLink fieldCode="PM" term="%2232531499%22">32531499)</searchLink><br />Toxicon. 2017 Nov;138:37-42. (PMID: <searchLink fieldCode="PM" term="%2228811248%22">28811248)</searchLink><br />Vet World. 2021 Aug;14(8):2187-2196. (PMID: <searchLink fieldCode="PM" term="%2234566338%22">34566338)</searchLink><br />PLoS Negl Trop Dis. 2021 Aug 17;15(8):e0009695. (PMID: <searchLink fieldCode="PM" term="%2234403421%22">34403421)</searchLink>
– Name: GrantInfo
  Label: Grant Information
  Group: Grant
  Data: #301744/2019-0 Brazilian Council for Scientific Research (CNPq)
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>Brucella abortus infection; ELISA-peptide; IgM and IgG epitope mapping; Omp-2a; brucellosis; diagnostic; transmembrane protein
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 0 (Immunoglobulin M)<br />0 (Immunoglobulin G)<br />0 (Porins)<br />0 (Bacterial Outer Membrane Proteins)<br />0 (Biomarkers)<br />0 (Antibodies, Bacterial)<br />0 (Epitopes, B-Lymphocyte)<br />0 (Antigens, Bacterial)
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: <i>Date Created: </i>20260626 <i>Date Completed: </i>20260626 <i>Latest Revision: </i>20260813
– Name: DateUpdate
  Label: Update Code
  Group: Date
  Data: 20260814
– Name: PubmedCentralID
  Label: PubMed Central ID
  Group: ID
  Data: PMC13299733
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.3390/ijms27125341
– Name: AN
  Label: PMID
  Group: ID
  Data: 42353060
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42353060
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.3390/ijms27125341
    Languages:
      – Code: eng
        Text: English
    Subjects:
      – SubjectFull: Biomarkers blood
        Type: general
      – SubjectFull: Antibodies, Bacterial immunology
        Type: general
      – SubjectFull: Epitopes, B-Lymphocyte immunology
        Type: general
      – SubjectFull: Antigens, Bacterial immunology
        Type: general
      – SubjectFull: Humans
        Type: general
      – SubjectFull: Enzyme-Linked Immunosorbent Assay
        Type: general
      – SubjectFull: Animals
        Type: general
      – SubjectFull: Cross Reactions
        Type: general
      – SubjectFull: Immunoglobulin M immunology
        Type: general
      – SubjectFull: Immunoglobulin G immunology
        Type: general
      – SubjectFull: Epitope Mapping methods
        Type: general
      – SubjectFull: Brucella abortus immunology
        Type: general
      – SubjectFull: Brucellosis diagnosis
        Type: general
      – SubjectFull: Brucellosis immunology
        Type: general
      – SubjectFull: Brucellosis microbiology
        Type: general
      – SubjectFull: Brucellosis blood
        Type: general
      – SubjectFull: Porins immunology
        Type: general
      – SubjectFull: Bacterial Outer Membrane Proteins immunology
        Type: general
    Titles:
      – TitleFull: IgM and IgG Epitope Mapping of the Porin Outer Membrane Protein-2a from Brucella abortus: Potential Biomarkers for Detecting Exposure to Brucellosis.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Noguera AF
      – PersonEntity:
          Name:
            NameFull: Lechuga GC
      – PersonEntity:
          Name:
            NameFull: Napoleão-Pêgo P
      – PersonEntity:
          Name:
            NameFull: Carvalho JPRS
      – PersonEntity:
          Name:
            NameFull: Gomes LR
      – PersonEntity:
          Name:
            NameFull: Carneiro da Silva A
      – PersonEntity:
          Name:
            NameFull: Melo Monnerat M
      – PersonEntity:
          Name:
            NameFull: da Silva FR
      – PersonEntity:
          Name:
            NameFull: De-Simone SG
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 13
              M: 06
              Text: 2026 Jun 13
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-electronic
              Value: 1422-0067
          Numbering:
            – Type: volume
              Value: 27
            – Type: issue
              Value: 12
          Titles:
            – TitleFull: International journal of molecular sciences
              Type: main
ResultId 1