Academic Journal
IgM and IgG Epitope Mapping of the Porin Outer Membrane Protein-2a from Brucella abortus: Potential Biomarkers for Detecting Exposure to Brucellosis.
| Τίτλος: | IgM and IgG Epitope Mapping of the Porin Outer Membrane Protein-2a from Brucella abortus: Potential Biomarkers for Detecting Exposure to Brucellosis. |
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| Συγγραφείς: | Noguera AF; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil., Lechuga GC; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil., Napoleão-Pêgo P; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil., Carvalho JPRS; Program of Post-Graduation on Science and Biotechnology, Department of Molecular and Cellular Biology, Biology Institute, Federal Fluminense University, Niterói 22040-036, Brazil., Gomes LR; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil., Carneiro da Silva A; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil., Melo Monnerat M; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil., da Silva FR; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil., De-Simone SG; Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.; Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil.; Program of Post-Graduation on Science and Biotechnology, Department of Molecular and Cellular Biology, Biology Institute, Federal Fluminense University, Niterói 22040-036, Brazil.; Program of Post-Graduation on Parasitic Biology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-900, Brazil. |
| Πηγή: | International journal of molecular sciences [Int J Mol Sci] 2026 Jun 13; Vol. 27 (12). Date of Electronic Publication: 2026 Jun 13. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI, [2000- |
| Ιατρικοί όροι (MeSH): | Immunoglobulin M*/immunology , Immunoglobulin G*/immunology , Epitope Mapping*/methods , Brucella abortus*/immunology , Brucellosis*/diagnosis , Brucellosis*/immunology , Brucellosis*/microbiology , Brucellosis*/blood , Porins*/immunology , Bacterial Outer Membrane Proteins*/immunology, Biomarkers/blood ; Antibodies, Bacterial/immunology ; Epitopes, B-Lymphocyte/immunology ; Antigens, Bacterial/immunology ; Humans ; Enzyme-Linked Immunosorbent Assay ; Animals ; Cross Reactions |
| Περίληψη: | Brucellosis is a globally prevalent zoonotic disease affecting both humans and animals. Its nonspecific clinical manifestations often complicate diagnosis, underscoring the need for reliable laboratory confirmation. Traditional serological assays, though widely used, suffer from limitations such as inconsistent sensitivity and false-positive results. To address these challenges, this study mapped IgM and IgG epitopes of the Brucella Omp-2a protein using sera from infected patients. Epitope identification was performed through SPOT synthesis on cellulose membranes, followed by assessment of potential cross-reactivity using peptide database analysis and ELISA validation. Three major IgM and seven IgG linear B-cell epitopes were identified, six of which demonstrated strong reactivity in peptide-ELISA. Importantly, no significant cross-reactivity with proteins from other human pathogens was detected. Two chimeric multi-epitope peptides, composed of 50 and 60 amino acids and integrating Brucella-specific IgM and IgG epitopes, exhibited excellent diagnostic performance in ELISA, achieving near 100% sensitivity and specificity. These findings support the potential of synthetic peptides as reliable and cost-effective alternatives to native antigens in serological assays. Further validation in larger, geographically diverse cohorts will be essential to confirm their diagnostic robustness and facilitate their integration into routine brucellosis diagnostics. |
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| Grant Information: | #301744/2019-0 Brazilian Council for Scientific Research (CNPq) |
| Contributed Indexing: | Keywords: Brucella abortus infection; ELISA-peptide; IgM and IgG epitope mapping; Omp-2a; brucellosis; diagnostic; transmembrane protein |
| Substance Nomenclature: | 0 (Immunoglobulin M) 0 (Immunoglobulin G) 0 (Porins) 0 (Bacterial Outer Membrane Proteins) 0 (Biomarkers) 0 (Antibodies, Bacterial) 0 (Epitopes, B-Lymphocyte) 0 (Antigens, Bacterial) |
| Entry Date(s): | Date Created: 20260626 Date Completed: 20260626 Latest Revision: 20260813 |
| Update Code: | 20260814 |
| PubMed Central ID: | PMC13299733 |
| DOI: | 10.3390/ijms27125341 |
| PMID: | 42353060 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1422-0067 |
|---|---|
| DOI: | 10.3390/ijms27125341 |