Academic Journal
Purification, Amino Acid Sequence, and Structural Features of a Novel Expansin-like A from the Seeds of Canihua (Chenopodium pallidicaule Aellen).
| Τίτλος: | Purification, Amino Acid Sequence, and Structural Features of a Novel Expansin-like A from the Seeds of Canihua (Chenopodium pallidicaule Aellen). |
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| Συγγραφείς: | Ragucci S; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DiSTABiF), University of Campania 'Luigi Vanvitelli', Via Vivaldi 43, 81100 Caserta, Italy., Campanile MG; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DiSTABiF), University of Campania 'Luigi Vanvitelli', Via Vivaldi 43, 81100 Caserta, Italy., Iglesias R; Department of Biochemistry and Molecular Biology and Physiology, Faculty of Sciences, University of Valladolid, 47011 Valladolid, Spain., Landi N; Institute of Crystallography, National Research Council, Via Vivaldi 43, 81100 Caserta, Italy., Gay CC; Laboratory of Protein Research, Institute of Basic and Applied Chemistry of Northeast Argentina (UNNE-CONICET), Faculty of Exact and Natural Sciences and Surveying, Corrientes 3400, Argentina., Oliver A; Institute of Biostructures and Bioimaging, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy., Khan R; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DiSTABiF), University of Campania 'Luigi Vanvitelli', Via Vivaldi 43, 81100 Caserta, Italy., Citores L; Department of Biochemistry and Molecular Biology and Physiology, Faculty of Sciences, University of Valladolid, 47011 Valladolid, Spain., Ferreras JM; Department of Biochemistry and Molecular Biology and Physiology, Faculty of Sciences, University of Valladolid, 47011 Valladolid, Spain., Di Maro A; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DiSTABiF), University of Campania 'Luigi Vanvitelli', Via Vivaldi 43, 81100 Caserta, Italy. |
| Πηγή: | International journal of molecular sciences [Int J Mol Sci] 2026 Jun 09; Vol. 27 (12). Date of Electronic Publication: 2026 Jun 09. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI, [2000- |
| Ιατρικοί όροι (MeSH): | Plant Proteins*/chemistry , Plant Proteins*/isolation & purification , Plant Proteins*/genetics , Plant Proteins*/metabolism , Seeds*/chemistry , Seeds*/metabolism , Seeds*/genetics, Amino Acid Sequence ; Phylogeny ; Models, Molecular |
| Περίληψη: | Expansin-like A (EXLA) proteins belong to one of the four main families within the expansin superfamily, a group of plant proteins essential for cell wall loosening. Here, we report, for the first time, the purification of a novel EXLA, named cpEXLA, from canihua seeds. cpEXLA (yield ~0.16 mg per 100 g of seeds) is a 29 kDa glycoprotein with a high melting temperature (Tm of 86.75 ± 1.06 °C). Elucidation of its primary structure reveals that the mature protein consists of 246 amino acids, ten of which are cysteine residues forming five disulphide bridges. Structural studies based on 3D model prediction reveal the presence of N- and C-terminal domains, which are typical of EXLAs and rich in β-sheets, as confirmed by circular dichroism (CD) spectroscopy. Furthermore, comparative analysis of amino acid sequences between cpEXLA and 219 similar EXLAs, retrieved from dicotyledonous genomes and transcriptomes, identified eighteen invariant amino acid residues: eleven in the N-terminal domain and seven in the C-terminal domain. Finally, phylogenetic analysis of EXLAs in dicotyledonous species shows a close relationship with other EXLAs from the Amaranthaceae family, confirming that EXLA proteins are highly conserved among dicotyledonous plants. Overall, cpEXLA represents an intriguing native tool for studying cell wall evolution and the functional role of EXLAs. |
| Grant Information: | Starting Grants 2025 'BioPro' (CUP: B63C25001050005) University of Campania Luigi Vanvitelli; ITACA.SB Project no. IR0000009 MUR-Italy; GIR ProtIBio VA033G19 Consejería de Educación, Junta de Castilla y León |
| Contributed Indexing: | Keywords: amino acid sequence; canihua seeds; cell wall loosening; expansin; molecular evolution; protein purification |
| Substance Nomenclature: | 0 (Plant Proteins) 0 (expansin protein, plant) |
| Entry Date(s): | Date Created: 20260626 Date Completed: 20260626 Latest Revision: 20260726 |
| Update Code: | 20260726 |
| PubMed Central ID: | PMC13299222 |
| DOI: | 10.3390/ijms27125213 |
| PMID: | 42352938 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1422-0067 |
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| DOI: | 10.3390/ijms27125213 |