Academic Journal
Genomic characterisation and global phylogenomic placement of a high-level azithromycin (HL-AziR) and fluoroquinolone-resistant Neisseria gonorrhoeae ST9363 strain from Chile.
| Τίτλος: | Genomic characterisation and global phylogenomic placement of a high-level azithromycin (HL-AziR) and fluoroquinolone-resistant Neisseria gonorrhoeae ST9363 strain from Chile. |
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| Συγγραφείς: | Aguayo-Reyes A; Grupo de Estudio de Enfermedades Infecciosas (GrEEn-UdeC), Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepción, Concepción, Chile; Grupo de Estudio en Resistencia Antimicrobiana (GRAM), Universidad de Concepción, Concepción, Chile; Laboratorio Clínico, Hospital Clínico Regional de Concepción Dr. Guillermo Grant Benavente (HGGB), Concepción, Chile., Quezada-Aguiluz M; Grupo de Estudio de Enfermedades Infecciosas (GrEEn-UdeC), Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepción, Concepción, Chile; Grupo de Estudio en Resistencia Antimicrobiana (GRAM), Universidad de Concepción, Concepción, Chile., Chabouty-García H; Laboratorio Clínico, Hospital Clínico Regional de Concepción Dr. Guillermo Grant Benavente (HGGB), Concepción, Chile., Gonzalez-Muñoz P; Grupo de Estudio de Enfermedades Infecciosas (GrEEn-UdeC), Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepción, Concepción, Chile; Laboratorio de Investigación en Agentes Antibacterianos (LIAA), Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile; Departamento de Ciencias Biológicas y Químicas, Facultad de Medicina y Ciencia, Universidad San Sebastián, Concepción, Chile., Ilabaca-Carrasco F; Grupo de Estudio en Resistencia Antimicrobiana (GRAM), Universidad de Concepción, Concepción, Chile; Laboratorio de Investigación en Agentes Antibacterianos (LIAA), Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile., Morales-León F; Unidad de Paciente Crítico (UPC), Hospital Las Higueras, Talcahuano, Chile., Matus-Köhler M; Grupo de Estudio en Resistencia Antimicrobiana (GRAM), Universidad de Concepción, Concepción, Chile; Laboratorio de Investigación en Agentes Antibacterianos (LIAA), Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile., Fuller S; Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile., Aguilera F; Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile., Neil M; Norwich Medical School, University of East Anglia, Norwich, UK; Metabolic Health Research Department, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, UK; Centre for Microbial Interactions, Norwich Research Park, Norwich, UK., Evans BA; Norwich Medical School, University of East Anglia, Norwich, UK; Metabolic Health Research Department, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, UK; Centre for Microbial Interactions, Norwich Research Park, Norwich, UK., González-Rocha G; Grupo de Estudio en Resistencia Antimicrobiana (GRAM), Universidad de Concepción, Concepción, Chile; Laboratorio de Investigación en Agentes Antibacterianos (LIAA), Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile., Opazo-Capurro A; Grupo de Estudio en Resistencia Antimicrobiana (GRAM), Universidad de Concepción, Concepción, Chile; Laboratorio de Investigación en Agentes Antibacterianos (LIAA), Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile. Electronic address: andopazo@udec.cl. |
| Πηγή: | Journal of global antimicrobial resistance [J Glob Antimicrob Resist] 2026 Sep; Vol. 50, pp. 91-94. Date of Electronic Publication: 2026 Jun 24. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer Country of Publication: Netherlands NLM ID: 101622459 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-7173 (Electronic) Linking ISSN: 22137165 NLM ISO Abbreviation: J Glob Antimicrob Resist Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam : Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer |
| Ιατρικοί όροι (MeSH): | Neisseria gonorrhoeae*/genetics , Neisseria gonorrhoeae*/drug effects , Neisseria gonorrhoeae*/classification , Neisseria gonorrhoeae*/isolation & purification , Azithromycin*/pharmacology , Fluoroquinolones*/pharmacology , Anti-Bacterial Agents*/pharmacology , Drug Resistance, Multiple, Bacterial*/genetics, Gonorrhea/microbiology ; Tetracycline/pharmacology ; Phylogeny ; Humans ; Chile ; Microbial Sensitivity Tests ; Whole Genome Sequencing ; Genome, Bacterial ; Multilocus Sequence Typing ; Third Generation Cephalosporins ; Drug Resistance, Bacterial |
| Περίληψη: | Background: Antibiotic-resistant Neisseria gonorrhoeae (Ng) is a WHO priority pathogen and represents a serious public health threat. This study reports the first Chilean Ng ST9363 isolate (GC11/23) exhibiting dual high-level resistance to azithromycin and fluoroquinolone, recovered from a urethral discharge sample in July 2023. Methods: Antimicrobial susceptibility was assessed using disk diffusion and MIC strip tests. Whole-genome sequencing (WGS) was performed using a hybrid approach, followed by annotation using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). In silico analyses included Staramr, MLST and NG-STAR. Phylogenetic analysis was performed on the core genome of 2417 global ST9363 Ng isolates using IQ-TREE and visualised with TreeViewer. Results: GC11/23 exhibited resistance to ciprofloxacin, tetracycline, penicillin and azithromycin, while remaining susceptible only to ceftriaxone and spectinomycin. The isolate belonged to ST9363 and NG-STAR 3194. Mutations associated with fluoroquinolone resistance were identified in gyrA and parC, while high-level azithromycin resistance (HL-AziR) was linked to mutations in all four copies of the 23S rRNA gene, as well as in mtrD and mtrR. Additional resistance-associated alterations included an insertion (ins346D) in penA and a mutation in rpsJ, conferring resistance to penicillin and tetracycline, respectively. Phylogenetic analysis clustered GC11/23 with isolates from the USA and Europe collected in 2019. Conclusions: GC11/23 represents a multidrug-resistant Ng strain belonging to ST9363/NG-STAR 3194 and carrying mutations conferring resistance to fluoroquinolones, azithromycin, penicillin and tetracycline. Its phylogenetic placement within a European lineage highlights the global dissemination of HL-AziR and fluoroquinolone-resistant strains. (Copyright © 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved.) |
| Contributed Indexing: | Keywords: Fluoroquinolone-resistance; High-level azithromycin resistance; Neisseria gonorrhoeae; ST9363; WHO priority pathogen |
| Substance Nomenclature: | 83905-01-5 (Azithromycin) 0 (Fluoroquinolones) 0 (Anti-Bacterial Agents) F8VB5M810T (Tetracycline) 0 (Third Generation Cephalosporins) |
| Entry Date(s): | Date Created: 20260624 Date Completed: 20260923 Latest Revision: 20260923 |
| Update Code: | 20260924 |
| DOI: | 10.1016/j.jgar.2026.06.008 |
| PMID: | 42342119 |
| Βάση Δεδομένων: | MEDLINE |
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