Academic Journal
Staged microbial consortium valorizes cattle manure wastewater and promotes maize growth.
| Τίτλος: | Staged microbial consortium valorizes cattle manure wastewater and promotes maize growth. |
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| Συγγραφείς: | Wang X; College of Agronomy, Henan University of Science and Technology, Luoyang 471003 Henan, China., Cao J; College of Agronomy, Henan University of Science and Technology, Luoyang 471003 Henan, China., Ni L; College of Agronomy, Henan University of Science and Technology, Luoyang 471003 Henan, China., Zhao H; College of Agronomy, Henan University of Science and Technology, Luoyang 471003 Henan, China., Zhou L; College of Agronomy, Henan University of Science and Technology, Luoyang 471003 Henan, China., Wang Y; College of Agronomy, Henan University of Science and Technology, Luoyang 471003 Henan, China. |
| Πηγή: | Journal of applied microbiology [J Appl Microbiol] 2026 Jul 06; Vol. 137 (7). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2022- : Oxford : Oxford University Press Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997- |
| Ιατρικοί όροι (MeSH): | Manure*/microbiology , Manure*/analysis , Zea mays*/growth & development , Zea mays*/microbiology , Zea mays*/metabolism , Wastewater*/microbiology , Microbial Consortia*, Ammonia/metabolism ; Bacteria/metabolism ; Bacteria/genetics ; Nitrogen/metabolism ; Animals ; Cattle ; Nitrification ; Fermentation |
| Περίληψη: | Aims: Ammonia-oxidizing bacteria play important roles in nitrogen transformation and agricultural-waste valorization; however, their large-scale application is often limited by high cultivation costs and insufficient functional stability. This study aimed to develop a low-cost and stable microbial consortium for converting cattle manure wastewater into a bioactive fermentation broth and to investigate its underlying mechanisms. Methods and Results: The heterotrophic ammonia-oxidizing bacterium Ensifer sp. S2-8-1 was used as the core strain, and Bacillus subtilis was employed as the auxiliary strain. Three cultivation systems were compared: S2-8-1 monoculture, simultaneous co-culture, and a staged consortium (SYC). Among them, SYC exhibited the best overall performance, maintaining the highest viable bacterial density and reaching 3.93 × 107 CFU ml-1 on day 12. In a soil-maize pot experiment, SYC significantly enhanced rhizosphere nitrification, increased NO3--N availability, and promoted maize growth, resulting in a 41.4% increase in whole-plant dry matter compared with the water control. The consortium also improved photosynthetic performance and cytokinin accumulation. Comparative metatranscriptomic analysis revealed significant upregulation of heterotrophic ammonia oxidation-related genes (CYP450) and cytokinin-related genes (miaA and LOG) in S2-8-1 under SYC conditions, suggesting coordinated activation of nitrogen transformation and plant growth-promoting signaling pathways. Conclusions: The staged "pre-modification followed by synergy" consortium effectively converts cattle manure wastewater into a stable and bioactive fermentation broth, enhancing both microbial biomass production and plant growth-promoting activity. This strategy provides a scalable approach for livestock wastewater valorization and the development of compound microbial fertilizers. (© The Author(s) 2026. Published by Oxford University Press on behalf of Applied Microbiology International. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com.) |
| Grant Information: | U1304326 National Natural Science Foundation of China; 242300421242 Natural Science Foundation of Henan Provincial |
| Contributed Indexing: | Keywords: Bacillus subtilis; ammonia-oxidizing bacterium S2-8-1; crop growth promotion; cytokinin; metatranscriptomics; nitrification; wastewater treatment |
| Substance Nomenclature: | 0 (Manure) 0 (Wastewater) 7664-41-7 (Ammonia) N762921K75 (Nitrogen) |
| Entry Date(s): | Date Created: 20260624 Date Completed: 20260722 Latest Revision: 20260723 |
| Update Code: | 20260723 |
| DOI: | 10.1093/jambio/lxag151 |
| PMID: | 42341825 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1365-2672 |
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| DOI: | 10.1093/jambio/lxag151 |