Academic Journal
Development and validation of a high-performance thin-layer chromatographic method for the quantitative analysis of polymethoxyflavones in citrus by-products.
| Τίτλος: | Development and validation of a high-performance thin-layer chromatographic method for the quantitative analysis of polymethoxyflavones in citrus by-products. |
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| Συγγραφείς: | Sharma A; Division of Food and Nutritional Biotechnology, National Agri-Food Biotechnology Institute (NABI), Sector-81, Mohali 140306, Punjab, India., Rani A; Division of Food and Nutritional Biotechnology, National Agri-Food Biotechnology Institute (NABI), Sector-81, Mohali 140306, Punjab, India., Nile SH; Division of Food and Nutritional Biotechnology, National Agri-Food Biotechnology Institute (NABI), Sector-81, Mohali 140306, Punjab, India. Electronic address: shivraj.nile@nabi.res.in. |
| Πηγή: | Journal of chromatography. A [J Chromatogr A] 2026 Aug 30; Vol. 1783, pp. 467196. Date of Electronic Publication: 2026 Jun 19. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9318488 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3778 (Electronic) Linking ISSN: 00219673 NLM ISO Abbreviation: J Chromatogr A Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam ; New York : Elsevier, 1993- |
| Ιατρικοί όροι (MeSH): | Citrus*/chemistry , Flavones*/analysis , Plant Extracts*/chemistry, Chromatography, Thin Layer/methods ; Chromatography, High Pressure Liquid/methods ; Reproducibility of Results ; Linear Models |
| Περίληψη: | Citrus peel and pomace, major byproducts of citrus processing, are rich in polymethoxyflavones (PMFs), such as nobiletin, tangeretin, and sinensetin. In this study, a cost-effective and reliable high-performance thin-layer chromatography (HPTLC) method was developed and validated for the simultaneous quantification of these PMFs in eleven citrus waste varieties. Separation was achieved on silica gel 60 F₂₅₄ plates using an optimized mobile phase of hexane:1-butanol: formic acid (80:20:1, v/v/v), with densitometric detection at 254 nm. The method exhibited excellent linearity over 0.078-2.50 µg band⁻¹ (R² = 0.9912-0.9986), high precision (intra- and interday %RSD < 2%), satisfactory recoveries (98.14%-100.97%), and robustness against deliberate chromatographic variations, in accordance with International Council for Harmonization (ICH) guidelines. The application of the method to eleven citrus waste varieties revealed pronounced interspecies variability in PMF content. Overall, the validated method provides a rapid, reproducible, and low-solvent analytical platform suitable for routine analysis and high-throughput analytical screening of citrus processing byproducts. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Citrus byproducts; HPTLC; Method validation; Phytochemical analysis; Polymethoxyflavones |
| Substance Nomenclature: | 0 (Flavones) 0 (Plant Extracts) |
| Entry Date(s): | Date Created: 20260624 Date Completed: 20260629 Latest Revision: 20260629 |
| Update Code: | 20260630 |
| DOI: | 10.1016/j.chroma.2026.467196 |
| PMID: | 42341508 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-3778 |
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| DOI: | 10.1016/j.chroma.2026.467196 |