Academic Journal
Biosynthesis of cerium and gadolinium nanoparticles by Pseudomonas putida KT2440 enables sustainable rare earth elements utilization.
| Τίτλος: | Biosynthesis of cerium and gadolinium nanoparticles by Pseudomonas putida KT2440 enables sustainable rare earth elements utilization. |
|---|---|
| Συγγραφείς: | García-García P; Biotechnology Department, Centro de Investigaciones Biológicas Margarita Salas-CSIC, Madrid, Spain.; Biotechnology Departament, Centro de Investigaciones Biológicas-CSIC, Madrid, Spain., Alonso-Fernandes E; Biotechnology Department, Centro de Investigaciones Biológicas Margarita Salas-CSIC, Madrid, Spain., Serrano-Pelejero C; Biotechnology Department, Centro de Investigaciones Biológicas Margarita Salas-CSIC, Madrid, Spain., González-Vega A; Biotechnology Department, Centro de Investigaciones Biológicas Margarita Salas-CSIC, Madrid, Spain., Castro L; Department of Material Science and Metallurgical Engineering, Facultad de Químicas, Universidad Complutense de Madrid, Madrid, Spain., Rodríguez A; Biotechnology Department, Centro de Investigaciones Biológicas Margarita Salas-CSIC, Madrid, Spain., Díaz E; Biotechnology Department, Centro de Investigaciones Biológicas Margarita Salas-CSIC, Madrid, Spain., Carmona M; Biotechnology Department, Centro de Investigaciones Biológicas Margarita Salas-CSIC, Madrid, Spain. mcarmona@cib.csic.es. |
| Πηγή: | Scientific reports [Sci Rep] 2026 Jun 23; Vol. 16 (1). Date of Electronic Publication: 2026 Jun 23. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Nature Publishing Group, copyright 2011- |
| Ιατρικοί όροι (MeSH): | Pseudomonas putida*/metabolism , Gadolinium*/metabolism , Gadolinium*/chemistry , Cerium*/metabolism , Cerium*/chemistry , Metals, Rare Earth*/metabolism , Metal Nanoparticles*/chemistry , Metal Nanoparticles*/ultrastructure, Nanoparticles/chemistry |
| Περίληψη: | Lanthanides (Ln), a group of 15 rare earth elements (REEs), are critical for advanced technologies, although their conventional extraction and processing are environmentally unsustainable. Here, we present a microbial platform based on Pseudomonas putida KT2440 for the eco-friendly recovery and transformation of Ln, introducing a key methodological innovation: the use of a resting cell system to circumvent the pervasive issue of abiotic lanthanide-phosphate precipitation. This approach enables controlled investigation of Ln biomineralization under mild conditions. Mechanistically, the results showed that Ln recovery proceeds via rapid cell-surface adsorption, followed by surface-templated nucleation and extracellular mineralization. This process leads to the formation of well-defined biogenic nanoparticles primary identified as CePO₄ and GdPO₄. Structural analyses reveal nanorod morphologies, while functional characterization shows that CePO₄ nanoparticles retain photoluminescent properties and GdPO₄ nanoparticles preserve paramagnetic behavior. Compared to conventional chemical synthesis, this biosynthetic strategy eliminates toxic reagents and energy-intensive steps, yielding biocompatible materials with controlled size and morphology. Our findings establish P. putida KT2440 as an efficient and sustainable platform for Ln recovery and functional nanoparticle production, providing a foundation for scalable green alternatives to traditional Ln processing. (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Competing interests: The authors declare no competing interests. |
| References: | Front Microbiol. 2022 Mar 17;13:820327. (PMID: 35369483) Curr Issues Mol Biol. 2019;33:101-116. (PMID: 31166187) RSC Adv. 2024 Jun 13;14(27):18978-19000. (PMID: 38873547) Proc Natl Acad Sci U S A. 1981 Dec;78(12):7458-62. (PMID: 6950388) Chem Rev. 2018 Jan 24;118(2):801-838. (PMID: 28876904) mSphere. 2018 Aug 29;3(4):. (PMID: 30158283) ACS Omega. 2020 Jun 04;5(23):14180-14185. (PMID: 32566886) Adv Mater. 2023 Aug;35(33):e2303457. (PMID: 37243571) mSphere. 2024 Oct 29;9(10):e0068524. (PMID: 39291981) Microb Biotechnol. 2023 May;16(5):931-946. (PMID: 36682039) Front Microbiol. 2019 Jan 30;10:81. (PMID: 30761115) Bioresour Technol. 2025 Sep;432:132680. (PMID: 40378972) Environ Sci Pollut Res Int. 2017 Oct;24(29):22764-22789. (PMID: 27722986) mBio. 2017 Jun 27;8(3):. (PMID: 28655819) Microb Cell Fact. 2017 Jun 13;16(1):106. (PMID: 28610636) Int J Mol Sci. 2023 Mar 27;24(7):. (PMID: 37047269) Environ Microbiol. 2002 Dec;4(12):799-808. (PMID: 12534463) Chem Soc Rev. 2006 Jun;35(6):524-33. (PMID: 16729146) Proc Natl Acad Sci U S A. 2021 May 4;118(18):. (PMID: 33903247) Ann Med Exp Biol Fenn. 1958;36(Supp 6):1-54. (PMID: 13595352) Microb Biotechnol. 2019 Sep;12(5):845-848. (PMID: 31199068) Metab Eng. 2018 Nov;50:142-155. (PMID: 29758287) Appl Environ Microbiol. 2021 Jun 11;87(13):e0314420. (PMID: 33893117) Ecotoxicol Environ Saf. 2025 Jan 01;289:117494. (PMID: 39647373) Angew Chem Int Ed Engl. 2005 Jan 14;44(4):576-9. (PMID: 15612077) Nanomedicine. 2010 Dec;6(6):738-44. (PMID: 20553964) J Nanobiotechnology. 2021 Mar 26;19(1):86. (PMID: 33771172) Bioorg Chem. 2024 Feb;143:107040. (PMID: 38141331) ChemSusChem. 2013 Nov;6(11):2045-55. (PMID: 24009098) Environ Toxicol Chem. 2015 Aug;34(8):1711-9. (PMID: 25772589) Nanomaterials (Basel). 2020 Feb 28;10(3):. (PMID: 32121089) Microb Biotechnol. 2023 May;16(5):915-930. (PMID: 36366868) J Hazard Mater. 2022 Mar 15;426:127844. (PMID: 34838363) Environ Microbiol. 2020 Jan;22(1):255-269. (PMID: 31657101) J Biol Chem. 2000 Oct 6;275(40):31061-8. (PMID: 10913128) Adv Microb Physiol. 2022;81:1-24. (PMID: 36167440) RSC Adv. 2023 Jun 13;13(26):17787-17811. (PMID: 37323462) Microb Biotechnol. 2024 Jun;17(6):e14503. (PMID: 38829373) Biomater Adv. 2025 Jan;166:214063. (PMID: 39476683) Front Microbiol. 2022 Mar 21;13:855077. (PMID: 35387087) J Nanobiotechnology. 2024 Oct 18;22(1):637. (PMID: 39420353) |
| Contributed Indexing: | Keywords: Biomining; Biotechnology; Circular economy; Lanthanides; Metallic nanoparticle; Rare earth elements |
| Substance Nomenclature: | AU0V1LM3JT (Gadolinium) 30K4522N6T (Cerium) 0 (Metals, Rare Earth) |
| Entry Date(s): | Date Created: 20260623 Date Completed: 20260915 Latest Revision: 20260917 |
| Update Code: | 20260917 |
| PubMed Central ID: | PMC13575118 |
| DOI: | 10.1038/s41598-026-59096-4 |
| PMID: | 42337336 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2045-2322 |
|---|---|
| DOI: | 10.1038/s41598-026-59096-4 |