Academic Journal
Morfo-anatomical insights into the germination and protocorm growth of the endangered Vanilla lindmaniana (Orchidaceae).
| Τίτλος: | Morfo-anatomical insights into the germination and protocorm growth of the endangered Vanilla lindmaniana (Orchidaceae). |
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| Συγγραφείς: | Campos JCM; Plant Micropropagation Laboratory, Department of Botany, Federal University of Paraná, Curitiba, Paraná, Brazil., Stefanello CA; Plant Micropropagation Laboratory, Department of Botany, Federal University of Paraná, Curitiba, Paraná, Brazil.; Graduation Program in Botany, Federal University of Paraná, Curitiba, Paraná, Brazil., Moraes VWP; Plant Micropropagation Laboratory, Department of Botany, Federal University of Paraná, Curitiba, Paraná, Brazil., Pansarin ER; FFCLRP, Department of Biology, University of São Paulo, Ribeirão Preto, São Paulo, Brazil., Pacheco de Freitas Fraga H; Plant Micropropagation Laboratory, Department of Botany, Federal University of Paraná, Curitiba, Paraná, Brazil. hugofraga@ufpr.br.; Graduation Program in Botany, Federal University of Paraná, Curitiba, Paraná, Brazil. hugofraga@ufpr.br. |
| Πηγή: | Biotechnology letters [Biotechnol Lett] 2026 Jun 22; Vol. 48 (4). Date of Electronic Publication: 2026 Jun 22. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8008051 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-6776 (Electronic) Linking ISSN: 01415492 NLM ISO Abbreviation: Biotechnol Lett Subsets: MEDLINE |
| Imprint Name(s): | Publication: 1999- : Dordrecht : Kluwer Academic Publishers Original Publication: [Kew, Eng., Science and Technology Letters] |
| Ιατρικοί όροι (MeSH): | Germination*/physiology , Seeds*/growth & development , Seeds*/anatomy & histology , Seeds*/ultrastructure , Vanilla*/growth & development , Vanilla*/anatomy & histology , Vanilla*/physiology, Plant Roots/growth & development ; Endangered Species ; Microscopy, Electron, Scanning |
| Περίληψη: | Despite their global importance, knowledge of Vanilla plant nutrition and physiology remains limited, particularly regarding germination processes that underpin species ecology, fruit attraction, and seed dispersal. This study elucidates the germination stages of the endangered Vanilla lindmaniana through detailed morpho-anatomical analyses. Mature seeds were disinfected, chemically scarified, and sown on BM1 medium under controlled conditions (30 °C, darkness). Protocorm development was characterized through stages at morphological and anatomical levels, utilizing light microscopy and scanning electron microscopy (SEM). Successful in vitro germination was achieved, with initiation at three months and a germination rate of 61.8%, alongside a protocorm rooting frequency of 93.86%. Germination was triggered by rupture of a specific seed coat layer, resulting in a "clam-like" split, concurrent with starch accumulation. Roots emerged at Stage IV, growing vigorously, while the shoot apex remained short; raphides were occasionally observed in the root cortex. This work presents a novel inoculation and germination procedure for mature V. lindmaniana seeds, highlighting BM1 medium as a suitable formulation for germination and early development. Understanding these mechanisms is vital for conservation efforts and genetic engineering, facilitating species propagation and advancing knowledge of the Vanilla life cycle. Our findings contribute essential insights into the early development stages of this rare species, supporting both its preservation and sustainable cultivation. (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Competing interests: The authors declare no competing interests. |
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| Grant Information: | PBA2022011000021 Paraná State Funding Agency Fundação Araucária |
| Contributed Indexing: | Keywords: BM1 culture medium; Conservation; Mature seeds; Micropropagation; Vanilloideae |
| Entry Date(s): | Date Created: 20260622 Date Completed: 20260622 Latest Revision: 20260726 |
| Update Code: | 20260726 |
| PubMed Central ID: | PMC13287153 |
| DOI: | 10.1007/s10529-026-03752-2 |
| PMID: | 42329378 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1573-6776 |
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| DOI: | 10.1007/s10529-026-03752-2 |