Academic Journal
Isoquinoline alkaloids from Stephania japonica var. discolor and their neuroprotective effects against OGD/R-induced oxidative injury.
| Title: | Isoquinoline alkaloids from Stephania japonica var. discolor and their neuroprotective effects against OGD/R-induced oxidative injury. |
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| Authors: | Du J; School of Traditional Chinese Materia Medica, Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, Shenyang Pharmaceutical University, Shenyang 110016, PR China., Xiao J; School of Traditional Chinese Materia Medica, Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, Shenyang Pharmaceutical University, Shenyang 110016, PR China., Zhou D; School of Traditional Chinese Materia Medica, Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, Shenyang Pharmaceutical University, Shenyang 110016, PR China., Chen G; School of Traditional Chinese Materia Medica, Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, Shenyang Pharmaceutical University, Shenyang 110016, PR China., Wang Y; School of Traditional Chinese Materia Medica, Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, Shenyang Pharmaceutical University, Shenyang 110016, PR China. Electronic address: wyj666163@163.com., Li N; School of Traditional Chinese Materia Medica, Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, Shenyang Pharmaceutical University, Shenyang 110016, PR China. Electronic address: liningsypharm@163.com. |
| Source: | Fitoterapia [Fitoterapia] 2026 Jul; Vol. 192, pp. 107338. Date of Electronic Publication: 2026 Jun 16. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 16930290R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6971 (Electronic) Linking ISSN: 0367326X NLM ISO Abbreviation: Fitoterapia Subsets: MEDLINE |
| Imprint Name(s): | Publication: 1999- : [Amsterdam] : Elsevier Original Publication: Milano : Inverni & Della Beffa S.p.A. |
| MeSH Terms: | Neuroprotective Agents*/pharmacology , Neuroprotective Agents*/isolation & purification , Alkaloids*/pharmacology , Alkaloids*/isolation & purification , Isoquinolines*/pharmacology , Isoquinolines*/isolation & purification , Oxidative Stress*/drug effects , Stephania*/chemistry , Reperfusion Injury*/drug therapy, Reactive Oxygen Species/metabolism ; Antioxidants/pharmacology ; Antioxidants/isolation & purification ; Cell Survival/drug effects ; Glucose/metabolism ; Plant Extracts/pharmacology ; Oxygen/metabolism ; Plants, Medicinal/chemistry ; Animals ; Rats ; PC12 Cells |
| Abstract: | Stephania japonica var. discolor is a traditional medicinal plant reported to contain alkaloids. In this study, eight isoquinoline alkaloids were isolated from the whole plant, including four previously undescribed compounds (1-3, 6). These compounds were evaluated for neuroprotective effects using an oxygen-glucose deprivation/reperfusion (OGD/R) model in PC-12 cells. Several of the isolated alkaloids significantly improved cell viability and reduced intracellular reactive oxygen species (ROS) levels under OGD/R conditions. These results suggest that the neuroprotective activity of these compounds may be closely associated with their antioxidant properties. This is the first study to report the neuroprotective activity of isoquinoline alkaloids from S. japonica var. discolor and to explore their preliminary antioxidant mechanisms. The findings expand the chemical profile of this plant and highlight promising natural product-derived leads for the development of therapeutic agents against ischemic stroke. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that may appear to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Anti-oxidative; Isoquinoline alkaloids; Neuroprotective; Stephania japonica var. discolor |
| Substance Nomenclature: | 0 (Neuroprotective Agents) 0 (Alkaloids) 0 (Isoquinolines) 0 (Reactive Oxygen Species) 0 (Antioxidants) IY9XDZ35W2 (Glucose) 0 (Plant Extracts) S88TT14065 (Oxygen) |
| SCR Organism: | Stephania japonica |
| Entry Date(s): | Date Created: 20260616 Date Completed: 20260624 Latest Revision: 20260624 |
| Update Code: | 20260625 |
| DOI: | 10.1016/j.fitote.2026.107338 |
| PMID: | 42303164 |
| Database: | MEDLINE |
| ISSN: | 1873-6971 |
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| DOI: | 10.1016/j.fitote.2026.107338 |