Academic Journal
HPV genotyping in head and neck cancer: Insights from a Colombian cohort.
| Τίτλος: | HPV genotyping in head and neck cancer: Insights from a Colombian cohort. |
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| Συγγραφείς: | Cruz-Romero SD; University Hospital Fundación Santa Fe de Bogotá, Pathology Department, Bogotá, Colombia., Ramos AE; Universidad Nacional de Colombia, Faculty of Science, Bogotá, Colombia., Hakim JA; University Hospital Fundación Santa Fe de Bogotá, Head and Neck Surgery, Bogotá, Colombia., Cubillos AE; University Hospital Fundación Santa Fe de Bogotá, Head and Neck Surgery, Bogotá, Colombia., Baldión AM; University Hospital Fundación Santa Fe de Bogotá, Pathology Department, Bogotá, Colombia., Perdomo S; International Agency for Research on Cancer (IARC/WHO), Genomic Epidemiology Branch, Lyon, France., Baldión PA; Universidad Nacional de Colombia, Dental School, Oral Health Department, Bogotá, Colombia. Electronic address: pabaldione@unal.edu.co., Rodríguez-Urrego PA; University Hospital Fundación Santa Fe de Bogotá, Pathology Department, Bogotá, Colombia. Electronic address: paula.rodriguez@fsfb.org.co. |
| Πηγή: | Brazilian journal of otorhinolaryngology [Braz J Otorhinolaryngol] 2026 Jul-Aug; Vol. 92 (4), pp. 101845. Date of Electronic Publication: 2026 Jun 16. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: E.N.T. Brazilian Society Country of Publication: Brazil NLM ID: 101207337 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1808-8686 (Electronic) Linking ISSN: 18088686 NLM ISO Abbreviation: Braz J Otorhinolaryngol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: São Paulo, SP, Brasil : E.N.T. Brazilian Society |
| Ιατρικοί όροι (MeSH): | Papillomavirus Infections*/virology , Papillomavirus Infections*/epidemiology , Human Papillomavirus Viruses*/genetics , Head and Neck Neoplasms*/virology , Squamous Cell Carcinoma of Head and Neck*/virology , Papillomaviridae*/genetics, Colombia/epidemiology ; Humans ; Female ; Genotype ; Male ; Cross-Sectional Studies ; Aged ; Middle Aged ; DNA, Viral ; Immunohistochemistry ; Polymerase Chain Reaction |
| Περίληψη: | Objective: To describe the frequency and distribution of Human Papillomavirus (HPV) infection in patients with Head and Neck Squamous Cell Carcinoma (HNSCC) treated at a tertiary hospital in Colombia. Methods: A descriptive cross-sectional study was conducted in 81 patients diagnosed with HNSCC between 2015 and 2021 at Fundación Santa Fe de Bogota, Colombia, as part of the Colombian cohort of the InterCHANGE and HEADSpace study. Formalin-Fixed Paraffin-Embedded (FFPE) tumor samples were analyzed for HPV DNA detection and genotyping. Fifty-nine samples were processed using the HPV-Direct Flow CHIP assay (Master Diagnóstica, Seville, Spain), and 22 with the INNO-LiPA HPV Genotyping Extra assay (Fujirebio, Gothenburg, Sweden). Both techniques identify high- and low-risk HPV genotypes through PCR amplification and reverse hybridization. Immunohistochemistry for p16 was performed in all cases using the anti-p16INK4a (E6H4 clone) antibody, considering strong nuclear and cytoplasmic staining in ≥ 70% of tumor cells as positive. Descriptive statistics included absolute and relative frequencies. Results: The mean age was 66-years (±11), and 64.2% were male. The oropharynx was the most frequent tumor location (50.6%, n = 41), followed by the oral cavity (35.8%, n = 29) and larynx (13.6%, n = 11). Overall, 51.9% (n = 42) of tumors were HPV DNA-positive. HPV infection was more frequent in oropharyngeal carcinomas (92.7%, n = 38) than in the oral cavity (6.9%, n = 2) or larynx (18.2%, n = 2). HPV-16 was the predominant genotype (79.4%), followed by HPV-35, HPV-18, and HPV-33. p16 overexpression was observed in 49.4% (n = 40) of cases, mainly in oropharyngeal carcinomas (87.8%, n = 36). Among HPV DNA-positive tumors, 92.5% (n = 37) were also p16-positive. Conclusions: This study demonstrates a high prevalence of HPV, predominantly HPV-16, in oropharyngeal carcinomas compared with other head and neck sites in our cohort. These results provide baseline data for Colombia and highlight the need for multicenter studies including more heterogeneous populations to guide future prevention and control strategies. Level of Evidence: 4 (According to the Oxford Centre for Evidence-Based Medicine, 2011). (Copyright © 2026. Published by Elsevier España S.L.U.) |
| Competing Interests: | Declaration of competing interest The authors declare no conflicts of interest related to this study. Where authors are identified as personnel of the International Agency for Research on Cancer/World Health Organisation, the authors alone are responsible for the views expressed in this article and they do not necessarily represent the decisions, policy or views of the International Agency for Research on Cancer/World Health Organisation. |
| Contributed Indexing: | Keywords: Colombia; Genotype; Head and neck squamous cell carcinoma; Human papillomavirus virus; Papillomavirus infections |
| Substance Nomenclature: | 0 (DNA, Viral) |
| Entry Date(s): | Date Created: 20260616 Date Completed: 20260714 Latest Revision: 20260726 |
| Update Code: | 20260726 |
| PubMed Central ID: | PMC13292555 |
| DOI: | 10.1016/j.bjorl.2026.101845 |
| PMID: | 42302422 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1808-8686 |
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| DOI: | 10.1016/j.bjorl.2026.101845 |