Academic Journal
Divergent impacts of additives on biological risks associated with antibiotic resistance genes during black soldier fly manure composting: A comparative study of biochar, humic acid, and tea residue amendments.
| Title: | Divergent impacts of additives on biological risks associated with antibiotic resistance genes during black soldier fly manure composting: A comparative study of biochar, humic acid, and tea residue amendments. |
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| Authors: | Wang N; College of Environment and Ecology, Hunan Agricultural University, Changsha, Hunan 410028, China; Yuelushan Laboratory, Hunan Agricultural University area, Changsha, Hunan 410000, China., Xu T; College of Environment and Ecology, Hunan Agricultural University, Changsha, Hunan 410028, China; Yuelushan Laboratory, Hunan Agricultural University area, Changsha, Hunan 410000, China., He W; College of Environment and Ecology, Hunan Agricultural University, Changsha, Hunan 410028, China; Yuelushan Laboratory, Hunan Agricultural University area, Changsha, Hunan 410000, China., Wu G; College of Environment and Ecology, Hunan Agricultural University, Changsha, Hunan 410028, China; Yuelushan Laboratory, Hunan Agricultural University area, Changsha, Hunan 410000, China., Li X; Hunan Haishang Environmental Biotechnology Co., Ltd., Changsha, Hunan 410205, China., Yang X; State Key Laboratory of Efficient Utilization of Arable Land in China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Yin H; Hunan Institute of Microbiology, Hunan Academy of Agricultural Sciences, Changsha 410009, China., Luo L; College of Environment and Ecology, Hunan Agricultural University, Changsha, Hunan 410028, China; Yuelushan Laboratory, Hunan Agricultural University area, Changsha, Hunan 410000, China., Zhang L; College of Environment and Ecology, Hunan Agricultural University, Changsha, Hunan 410028, China; Yuelushan Laboratory, Hunan Agricultural University area, Changsha, Hunan 410000, China., He L; Institute of Subtropical Agriculture, Chinese Academy of Science, Changsha, Hunan 410000, China., Chen A; College of Environment and Ecology, Hunan Agricultural University, Changsha, Hunan 410028, China; Yuelushan Laboratory, Hunan Agricultural University area, Changsha, Hunan 410000, China., Yu M; Xingzhi College, Zhejiang Normal University, Jinhua, Zhengjiang 321100, China., Huang H; College of Environment and Ecology, Hunan Agricultural University, Changsha, Hunan 410028, China; Yuelushan Laboratory, Hunan Agricultural University area, Changsha, Hunan 410000, China., Zhang J; College of Environment and Ecology, Hunan Agricultural University, Changsha, Hunan 410028, China; Yuelushan Laboratory, Hunan Agricultural University area, Changsha, Hunan 410000, China. Electronic address: jiachao.zhang@163.com. |
| Source: | Journal of hazardous materials [J Hazard Mater] 2026 Aug 01; Vol. 514, pp. 142721. Date of Electronic Publication: 2026 Jun 15. |
| Publication Type: | Journal Article; Comparative Study |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9422688 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3336 (Electronic) Linking ISSN: 03043894 NLM ISO Abbreviation: J Hazard Mater Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam : Elsevier |
| MeSH Terms: | Manure*/microbiology , Tea*/chemistry , Drug Resistance, Microbial*/genetics , Humic Substances* , Composting* , Charcoal*, Animals ; Diptera ; Genes, Bacterial ; Metals, Heavy |
| Abstract: | Black soldier fly (BSF) manure produced through biological conversion technology still raises safety concerns related to antibiotic resistance genes (ARGs) and heavy metal residues. Composting has emerged as a viable approach for mitigating the proliferation of ARGs within livestock manure matrices. This study evaluated the effects of biochar, humic acid, and tea residue on the fate of ARGs, mobile genetic elements (MGEs), heavy metal resistance genes (HMRGs), and virulence factors (VFs) during BSF manure composting. Results indicate that humic acid exhibits the strongest inhibitory effect on ARGs and MGEs, achieving a 95.6% removal rate for intI1 and reducing the abundance of MLS(B) genes by 80.5%. Biochar demonstrates the strongest suppression of HMRGs, substantially decreasing the abundance of genes such as arsC and copA. However, the abundance of certain genes, such as intl2, catB8, arsM and cusA, increased across all treatments. Although humic acid increased the overall abundance of VFs, it achieved a final suppression rate of 86.3% by the end of the composting process. Interactions between bacterial phyla during composting were predominantly positively correlated. Network analysis revealed that humic acid most significantly suppressed co-occurrence associations among ARGs. Environmental factors such as pH and organic matter were associatively linked to ARGs reduction through a pathway that sequentially involved bacterial abundance and VFs (indirect effect = -0.87), suggesting a potential mechanistic cascade that warrants further experimental validation. This study provides a theoretical framework for optimizing the BSF manure composting process while acknowledging both the potential and limitations of additive amendments. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Antibiotics resistance gene; Black soldier fly manure; Composting; Heavy metal resistance gene; Microorganism |
| Substance Nomenclature: | 0 (Humic Substances) 0 (Manure) 16291-96-6 (Charcoal) 0 (biochar) 0 (Tea) 0 (Metals, Heavy) |
| Entry Date(s): | Date Created: 20260616 Date Completed: 20260725 Latest Revision: 20260725 |
| Update Code: | 20260725 |
| DOI: | 10.1016/j.jhazmat.2026.142721 |
| PMID: | 42302364 |
| Database: | MEDLINE |
| ISSN: | 1873-3336 |
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| DOI: | 10.1016/j.jhazmat.2026.142721 |