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BMSC-targeting aptamer conjugation enhances bone delivery of a rice bran peptide to mitigate osteoporosis via eIF3L.

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Τίτλος: BMSC-targeting aptamer conjugation enhances bone delivery of a rice bran peptide to mitigate osteoporosis via eIF3L.
Συγγραφείς: Yin H; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Wan TF; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Rao SS; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., He ZH; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Liu ZZ; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China., Wang ZX; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China., Lin ZR; Department of Metabolism and Endocrinology, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China., Wang YY; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Jin L; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Liu YW; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Zhu GQ; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Gong JS; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Cao J; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Wang Z; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; Xiangya School of Nursing, Central South University, Changsha, Hunan, 410013, China., Tan YJ; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Qian YX; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Wang X; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China., Lin QL; Molecular Nutrition Branch, National Engineering Research Center of Rice and By-product Deep Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China., Liang Y; Molecular Nutrition Branch, National Engineering Research Center of Rice and By-product Deep Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China., Chen CY; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China., Xie H; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.
Πηγή: Materials today. Bio [Mater Today Bio] 2026 May 27; Vol. 38, pp. 103289. Date of Electronic Publication: 2026 May 27 (Print Publication: 2026).
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Elsevier Ltd Country of Publication: England NLM ID: 101757228 Publication Model: eCollection Cited Medium: Internet ISSN: 2590-0064 (Electronic) Linking ISSN: 25900064 NLM ISO Abbreviation: Mater Today Bio Subsets: PubMed not MEDLINE
Imprint Name(s): Original Publication: [London] : Elsevier Ltd., [2019]-
Περίληψη: Osteoporosis is frequently accompanied by a shift in the fate of bone marrow mesenchymal stromal cells (BMSCs) from osteogenesis toward adipogenesis, contributing to bone-fat imbalance and fragility fractures. Here, we evaluated a naturally occurring eight-amino acid rice bran-derived peptide, RBAP (KHNRGDEF), together with an aptamer-RBAP conjugate designed to improve bone accumulation via a previously identified BMSC-targeting nucleic acid aptamer. In ovariectomized mice, RBAP administered either preventively (early after ovariectomy) or therapeutically (after osteoporotic phenotypes had developed) preserved trabecular microarchitecture, improved mechanical properties, reduced marrow adiposity, and accelerated osteoporotic fracture healing. RBAP also conferred bone-protective effects in models of natural aging and disuse osteoporosis. In vitro, RBAP promoted osteogenic differentiation and suppressed adipogenic differentiation of primary BMSCs without detectably affecting osteoclastogenesis. Proteomic pull-down and functional screening identified eukaryotic translation initiation factor 3 subunit L (eIF3L) as a key RBAP-interacting protein, and genetic suppression of eIF3l attenuated RBAP-induced osteogenesis and blunted its bone protective capacity in vivo. Conjugation of RBAP to the BMSC-targeting aptamer increased BMSC uptake and bone tissue accumulation, thereby enhancing its pro-osteogenic and anti-osteoporotic effects at the same administered dose. Our study identifies RBAP as a bioactive peptide candidate for correcting bone-fat imbalance in osteoporosis and support aptamer conjugation as a modular strategy to improve bone delivery.
(© 2026 Published by Elsevier Ltd.)
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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Contributed Indexing: Keywords: Aptamer; Bone marrow mesenchymal stromal cells; Osteogenesis; Osteoporosis; Rice bran-derived peptide; eIF3L
Entry Date(s): Date Created: 20260615 Date Completed: 20260615 Latest Revision: 20260813
Update Code: 20260813
PubMed Central ID: PMC13255075
DOI: 10.1016/j.mtbio.2026.103289
PMID: 42293385
Βάση Δεδομένων: MEDLINE
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  Data: BMSC-targeting aptamer conjugation enhances bone delivery of a rice bran peptide to mitigate osteoporosis via eIF3L.
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  Data: <searchLink fieldCode="AU" term="%22Yin+H%22">Yin H</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22Wan+TF%22">Wan TF</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22Rao+SS%22">Rao SS</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22He+ZH%22">He ZH</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22Liu+ZZ%22">Liu ZZ</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22Wang+ZX%22">Wang ZX</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22Lin+ZR%22">Lin ZR</searchLink>; Department of Metabolism and Endocrinology, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China.<br /><searchLink fieldCode="AU" term="%22Wang+YY%22">Wang YY</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22Jin+L%22">Jin L</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22Liu+YW%22">Liu YW</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22Zhu+GQ%22">Zhu GQ</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22Gong+JS%22">Gong JS</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22Cao+J%22">Cao J</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22Wang+Z%22">Wang Z</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; 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Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22Lin+QL%22">Lin QL</searchLink>; Molecular Nutrition Branch, National Engineering Research Center of Rice and By-product Deep Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China.<br /><searchLink fieldCode="AU" term="%22Liang+Y%22">Liang Y</searchLink>; Molecular Nutrition Branch, National Engineering Research Center of Rice and By-product Deep Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China.<br /><searchLink fieldCode="AU" term="%22Chen+CY%22">Chen CY</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.<br /><searchLink fieldCode="AU" term="%22Xie+H%22">Xie H</searchLink>; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.
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  Data: <i>Original Publication</i>: [London] : Elsevier Ltd., [2019]-
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  Data: Osteoporosis is frequently accompanied by a shift in the fate of bone marrow mesenchymal stromal cells (BMSCs) from osteogenesis toward adipogenesis, contributing to bone-fat imbalance and fragility fractures. Here, we evaluated a naturally occurring eight-amino acid rice bran-derived peptide, RBAP (KHNRGDEF), together with an aptamer-RBAP conjugate designed to improve bone accumulation via a previously identified BMSC-targeting nucleic acid aptamer. In ovariectomized mice, RBAP administered either preventively (early after ovariectomy) or therapeutically (after osteoporotic phenotypes had developed) preserved trabecular microarchitecture, improved mechanical properties, reduced marrow adiposity, and accelerated osteoporotic fracture healing. RBAP also conferred bone-protective effects in models of natural aging and disuse osteoporosis. In vitro, RBAP promoted osteogenic differentiation and suppressed adipogenic differentiation of primary BMSCs without detectably affecting osteoclastogenesis. Proteomic pull-down and functional screening identified eukaryotic translation initiation factor 3 subunit L (eIF3L) as a key RBAP-interacting protein, and genetic suppression of eIF3l attenuated RBAP-induced osteogenesis and blunted its bone protective capacity in vivo. Conjugation of RBAP to the BMSC-targeting aptamer increased BMSC uptake and bone tissue accumulation, thereby enhancing its pro-osteogenic and anti-osteoporotic effects at the same administered dose. Our study identifies RBAP as a bioactive peptide candidate for correcting bone-fat imbalance in osteoporosis and support aptamer conjugation as a modular strategy to improve bone delivery.<br /> (© 2026 Published by Elsevier Ltd.)
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  Data: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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  Data: Acta Pharm Sin B. 2021 Aug;11(8):2416-2448. (PMID: <searchLink fieldCode="PM" term="%2234522593%22">34522593)</searchLink><br />Exp Cell Res. 2017 Mar 15;352(2):346-356. (PMID: <searchLink fieldCode="PM" term="%2228215635%22">28215635)</searchLink><br />Sci Adv. 2021 Nov 26;7(48):eabl6432. (PMID: <searchLink fieldCode="PM" term="%2234818042%22">34818042)</searchLink><br />Adv Drug Deliv Rev. 2025 Nov;226:115680. (PMID: <searchLink fieldCode="PM" term="%2240886894%22">40886894)</searchLink><br />J Agric Food Chem. 2020 Nov 4;68(44):12271-12283. (PMID: <searchLink fieldCode="PM" term="%2232942847%22">32942847)</searchLink><br />Protein Pept Lett. 2021;28(6):623-642. (PMID: <searchLink fieldCode="PM" term="%2233319654%22">33319654)</searchLink><br />Int J Mol Sci. 2022 Jan 26;23(3):. (PMID: <searchLink fieldCode="PM" term="%2235163342%22">35163342)</searchLink><br />Adv Pharm Bull. 2024 Mar;14(1):11-33. 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