Academic Journal
BMSC-targeting aptamer conjugation enhances bone delivery of a rice bran peptide to mitigate osteoporosis via eIF3L.
| Τίτλος: | BMSC-targeting aptamer conjugation enhances bone delivery of a rice bran peptide to mitigate osteoporosis via eIF3L. |
|---|---|
| Συγγραφείς: | Yin H; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Wan TF; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Rao SS; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., He ZH; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Liu ZZ; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China., Wang ZX; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China., Lin ZR; Department of Metabolism and Endocrinology, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China., Wang YY; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Jin L; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Liu YW; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Zhu GQ; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Gong JS; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Cao J; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Wang Z; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; Xiangya School of Nursing, Central South University, Changsha, Hunan, 410013, China., Tan YJ; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Qian YX; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China., Wang X; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China., Lin QL; Molecular Nutrition Branch, National Engineering Research Center of Rice and By-product Deep Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China., Liang Y; Molecular Nutrition Branch, National Engineering Research Center of Rice and By-product Deep Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China., Chen CY; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China., Xie H; Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.; Hunan Key Laboratory of Angiomedicine, Changsha, Hunan, 410008, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China. |
| Πηγή: | Materials today. Bio [Mater Today Bio] 2026 May 27; Vol. 38, pp. 103289. Date of Electronic Publication: 2026 May 27 (Print Publication: 2026). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Ltd Country of Publication: England NLM ID: 101757228 Publication Model: eCollection Cited Medium: Internet ISSN: 2590-0064 (Electronic) Linking ISSN: 25900064 NLM ISO Abbreviation: Mater Today Bio Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: [London] : Elsevier Ltd., [2019]- |
| Περίληψη: | Osteoporosis is frequently accompanied by a shift in the fate of bone marrow mesenchymal stromal cells (BMSCs) from osteogenesis toward adipogenesis, contributing to bone-fat imbalance and fragility fractures. Here, we evaluated a naturally occurring eight-amino acid rice bran-derived peptide, RBAP (KHNRGDEF), together with an aptamer-RBAP conjugate designed to improve bone accumulation via a previously identified BMSC-targeting nucleic acid aptamer. In ovariectomized mice, RBAP administered either preventively (early after ovariectomy) or therapeutically (after osteoporotic phenotypes had developed) preserved trabecular microarchitecture, improved mechanical properties, reduced marrow adiposity, and accelerated osteoporotic fracture healing. RBAP also conferred bone-protective effects in models of natural aging and disuse osteoporosis. In vitro, RBAP promoted osteogenic differentiation and suppressed adipogenic differentiation of primary BMSCs without detectably affecting osteoclastogenesis. Proteomic pull-down and functional screening identified eukaryotic translation initiation factor 3 subunit L (eIF3L) as a key RBAP-interacting protein, and genetic suppression of eIF3l attenuated RBAP-induced osteogenesis and blunted its bone protective capacity in vivo. Conjugation of RBAP to the BMSC-targeting aptamer increased BMSC uptake and bone tissue accumulation, thereby enhancing its pro-osteogenic and anti-osteoporotic effects at the same administered dose. Our study identifies RBAP as a bioactive peptide candidate for correcting bone-fat imbalance in osteoporosis and support aptamer conjugation as a modular strategy to improve bone delivery. (© 2026 Published by Elsevier Ltd.) |
| Competing Interests: | The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| References: | Acta Pharm Sin B. 2021 Aug;11(8):2416-2448. (PMID: 34522593) Exp Cell Res. 2017 Mar 15;352(2):346-356. (PMID: 28215635) Sci Adv. 2021 Nov 26;7(48):eabl6432. (PMID: 34818042) Adv Drug Deliv Rev. 2025 Nov;226:115680. (PMID: 40886894) J Agric Food Chem. 2020 Nov 4;68(44):12271-12283. (PMID: 32942847) Protein Pept Lett. 2021;28(6):623-642. (PMID: 33319654) Int J Mol Sci. 2022 Jan 26;23(3):. (PMID: 35163342) Adv Pharm Bull. 2024 Mar;14(1):11-33. (PMID: 38585454) Nanoscale. 2019 Nov 21;11(43):20884-20892. (PMID: 31660556) J Biol Chem. 2020 Apr 10;295(15):4870-4880. (PMID: 32127399) Foods. 2017 Apr 26;6(5):. (PMID: 28445415) J Clin Invest. 2015 Apr;125(4):1509-22. (PMID: 25751060) Calcif Tissue Int. 2022 May;110(5):592-604. (PMID: 33738515) Stem Cells Int. 2020 Sep 10;2020:8836258. (PMID: 32963550) Sci Adv. 2022 Apr 15;8(15):eabg8335. (PMID: 35417243) J Control Release. 2018 Oct 10;287:167-176. (PMID: 30145135) Pharmacol Ther. 2022 Sep;237:108168. (PMID: 35283172) Crit Rev Food Sci Nutr. 2017 Nov 22;57(17):3771-3780. (PMID: 27015585) Nat Commun. 2022 Mar 18;13(1):1453. (PMID: 35304471) Phytomedicine. 2024 Sep;132:155864. (PMID: 39032281) Nucleic Acids Res. 2026 Jan 14;54(2):. (PMID: 41556339) Bone Rep. 2020 Feb 05;12:100250. (PMID: 32090156) Bone Res. 2018 Mar 30;6:9. (PMID: 29619269) Biochem Biophys Res Commun. 2019 Dec 3;520(2):420-427. (PMID: 31607480) Nature. 2015 Jun 4;522(7554):111-4. (PMID: 25849773) Cell Commun Signal. 2020 Nov 4;18(1):175. (PMID: 33148274) Nutrients. 2018 Nov 12;10(11):. (PMID: 30424533) Cell Stem Cell. 2018 Aug 02;23(2):193-209.e5. (PMID: 30017591) Autophagy. 2021 Oct;17(10):2766-2782. (PMID: 33143524) Int J Mol Sci. 2022 Jan 27;23(3):. (PMID: 35163367) Adv Sci (Weinh). 2021 Dec;8(24):e2100808. (PMID: 34719888) Genes Dis. 2023 Sep 07;11(3):101075. (PMID: 38292169) Mater Today Bio. 2023 Nov 07;23:100854. (PMID: 38024846) Bone Res. 2019 Jun 26;7:18. (PMID: 31263627) Chem Soc Rev. 2019 Mar 4;48(5):1390-1419. (PMID: 30707214) RNA Biol. 2018 Jan 2;15(1):26-34. (PMID: 29099306) Stem Cells Int. 2014;2014:917168. (PMID: 25610471) Cell Rep Med. 2024 Aug 20;5(8):101657. (PMID: 38986622) Crit Rev Food Sci Nutr. 2018;58(13):2273-2284. (PMID: 28604060) Elife. 2019 Aug 15;8:. (PMID: 31414986) Oxid Med Cell Longev. 2021 Sep 29;2021:2637577. (PMID: 34630847) Lancet. 2019 Jan 26;393(10169):364-376. (PMID: 30696576) J Agric Food Chem. 2018 Jan 17;66(2):440-448. (PMID: 29276944) Bone Res. 2022 Feb 18;10(1):16. (PMID: 35181672) Dis Model Mech. 2020 Dec 1;13(11):. (PMID: 33033107) Annu Rev Med. 2020 Jan 27;71:277-288. (PMID: 31509477) Food Funct. 2021 Aug 21;12(16):7108-7125. (PMID: 34223585) World J Stem Cells. 2024 Feb 26;16(2):102-113. (PMID: 38455105) Elife. 2025 Mar 28;13:. (PMID: 40153305) Sci Adv. 2025 Apr 11;11(15):eadq5484. (PMID: 40203091) Nat Rev Mol Cell Biol. 2010 Feb;11(2):113-27. (PMID: 20094052) Nat Rev Rheumatol. 2009 Aug;5(8):442-7. (PMID: 19581903) Nat Genet. 2019 Apr;51(4):716-727. (PMID: 30833796) Nat Rev Drug Discov. 2017 Mar;16(3):181-202. (PMID: 27807347) Methods Mol Biol. 2022;2533:217-228. (PMID: 35796991) Curr Med Chem. 2020;27(13):2189-2219. (PMID: 30295183) PLoS Biol. 2025 Mar 11;23(3):e3003049. (PMID: 40067813) |
| Contributed Indexing: | Keywords: Aptamer; Bone marrow mesenchymal stromal cells; Osteogenesis; Osteoporosis; Rice bran-derived peptide; eIF3L |
| Entry Date(s): | Date Created: 20260615 Date Completed: 20260615 Latest Revision: 20260813 |
| Update Code: | 20260813 |
| PubMed Central ID: | PMC13255075 |
| DOI: | 10.1016/j.mtbio.2026.103289 |
| PMID: | 42293385 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2590-0064 |
|---|---|
| DOI: | 10.1016/j.mtbio.2026.103289 |