Neobacillus camarae sp. nov. a new bacterium isolated from the breast milk of a Senegalese mother breast-feeding a healthy child and genomic description of Neobacillus dielmonensis DSM 27844 FF4T and Neobacillus drentensis LMG 21831T.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Neobacillus camarae sp. nov. a new bacterium isolated from the breast milk of a Senegalese mother breast-feeding a healthy child and genomic description of Neobacillus dielmonensis DSM 27844 FF4^T and Neobacillus drentensis LMG 21831^T.
Συγγραφείς:	Sarr S; IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; Aix Marseille Univ, MEPHI, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France., Ndour O; IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; Aix Marseille Univ, MEPHI, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France., Mbow A; IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France., Beye M; IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; Assistance Publique-Hôpitaux de Marseille (APHM), 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France., Idrissa Abdoulaye A; IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; Aix Marseille Univ, MEPHI, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France., Bellali S; IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France., Alibar S; IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France., Semmar R; IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; Aix Marseille Univ, MEPHI, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France., Orain N; IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; Assistance Publique-Hôpitaux de Marseille (APHM), 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France., Diatta G; Aix Marseille Univ, IRD, MINES, Campus International IRD-UCAD, Dakar, Senegal., Valles C; IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; Aix Marseille Univ, MEPHI, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; Assistance Publique-Hôpitaux de Marseille (APHM), 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France., Thiakane C; ICP Saint Martin Health Centre, 99000, Dakar, Senegal., Tidjani Alou M; IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; Aix Marseille Univ, MEPHI, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France., Sokhna C; IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; Aix Marseille Univ, IRD, MINES, Campus International IRD-UCAD, Dakar, Senegal.; Aix Marseille Univ, RITMES, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France., Million M; IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France. matthieumillion@gmail.com.; Aix Marseille Univ, MEPHI, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France. matthieumillion@gmail.com.; Assistance Publique-Hôpitaux de Marseille (APHM), 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France. matthieumillion@gmail.com.
Πηγή:	Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2026 Jun 13; Vol. 119 (7). Date of Electronic Publication: 2026 Jun 13.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Springer Country of Publication: Netherlands NLM ID: 0372625 Publication Model: Electronic Cited Medium: Internet ISSN: 1572-9699 (Electronic) Linking ISSN: 00036072 NLM ISO Abbreviation: Antonie Van Leeuwenhoek Subsets: MEDLINE
Imprint Name(s):	Publication: 2004- : Berlin : Springer Original Publication: Wageningen, Netherland [etc.] Veenman [etc.]
Ιατρικοί όροι (MeSH):	Milk, Human/microbiology , Bacillaceae/genetics , Bacillaceae/isolation & purification , Bacillaceae/classification, RNA, Ribosomal, 16S/genetics ; DNA, Bacterial/genetics ; Humans ; Phylogeny ; Base Composition ; Female ; Breast Feeding ; Genome, Bacterial ; Senegal ; Sequence Analysis, DNA ; Bacterial Typing Techniques ; Nucleic Acid Hybridization ; Fatty Acids
Περίληψη:	As part of a comprehensive study investigating the breast milk microbiota of healthy and malnourished children through a culturomic approach, a novel bacterial strain belonging to the genus Neobacillus was isolated, identified, and characterized using a polyphasic strategy. This isolate, designated as strain Neobacillus camarae sp. nov. Marseille-QA0830^T, was recovered from the breast milk of a mother nursing a healthy infant. Strain Marseille-QA0830^T (= CSUR QA0830^T = CECT 31295^T) is a Gram-stain-positive, non-motile, non-spore-forming bacillus, facultatively anaerobic showing optimal growth under aerobic conditions but also capable of developing in anaerobic environment at temperatures ranging from 20 to 45 °C. With a genome size of 5.83 Mbp, strain Marseille-QA0830^T exhibits a G + C content of 42.3%. Phylogenetic analysis based on the 16S rRNA gene revealed a high sequence similarity (98.10%) between strain Marseille-QA0830^T and Neobacillus niacini NBRC 15566^T (= IFO15566). A significant genomic divergence was observed through digital DNA-DNA hybridization (dDDH), against Neobacillus dielmonensis with 26.2% similarity well below the 70% threshold for species delineation. At the same time, rpoB gene analysis indicated a distant relationship with Neobacillus drentensis LMG 21831^T (83.5% similarity). Collectively, these genomic data demonstrate that although strain Marseille-QA0830^T is phylogenetically closely related to N. niacini NBRC 15566^T based on 16S rRNA gene sequences, it remains distinct at the whole-genome and rpoB levels from previously described species within the Neobacillus nomenclature. These findings confirm that this isolate represents a unique and previously undescribed taxon. Consequently, we propose the description of the type strain Marseille-QA0830^T as a new species named Neobacillus camarae sp. nov. (© 2026. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Competing Interests:	Declarations. Conflict of interest: The authors declare no conflict of interest. Ethical approval: This collection was initiated as part of the study of the microbiota of breast milk in malnutrition following the granting of administrative authorisation from the Ethics and Scientific Committee of the Senegalese Ministry of Health and Social Action (MSAS) under no. 00000233/MSAS/CNERS/SP on 11 September 2022.
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Contributed Indexing:	Keywords: Neobacillus camarae sp. nov.; Breast milk; New species; Senegal; Severe acute malnutrition; Taxonogenomics
Substance Nomenclature:	0 (RNA, Ribosomal, 16S) 0 (DNA, Bacterial) 0 (Fatty Acids)
Entry Date(s):	Date Created: 20260613 Date Completed: 20260614 Latest Revision: 20260714
Update Code:	20260714
DOI:	10.1007/s10482-026-02359-x
PMID:	42288713
Βάση Δεδομένων:	MEDLINE

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ISSN:	1572-9699
DOI:	10.1007/s10482-026-02359-x