Academic Journal
Antimicrobial peptide SAAP-148 inhibits Helicobacter pylori and is associated with membrane disruption, biofilm suppression, and reduced cell-associated urease activity.
| Τίτλος: | Antimicrobial peptide SAAP-148 inhibits Helicobacter pylori and is associated with membrane disruption, biofilm suppression, and reduced cell-associated urease activity. |
|---|---|
| Συγγραφείς: | Wang Y; National Center for Clinical Laboratories, Beijing Hospital, National Center for Gerontology, National Clinical Research Center for Gerontology, The Key Laboratory of Geriatrics of NHC, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, P.R. China., Zhang R; National Center for Clinical Laboratories, Beijing Hospital, National Center for Gerontology, National Clinical Research Center for Gerontology, The Key Laboratory of Geriatrics of NHC, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, P.R. China., Gao Z; National Center for Clinical Laboratories, Beijing Hospital, National Center for Gerontology, National Clinical Research Center for Gerontology, The Key Laboratory of Geriatrics of NHC, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, P.R. China., Liu X; Department of Gastroenterology, Emergency General Hospital, Beijing 100028, China., Pian Y; National Center for Clinical Laboratories, Beijing Hospital, National Center for Gerontology, National Clinical Research Center for Gerontology, The Key Laboratory of Geriatrics of NHC, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, P.R. China. |
| Πηγή: | Journal of applied microbiology [J Appl Microbiol] 2026 Jun 03; Vol. 137 (6). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2022- : Oxford : Oxford University Press Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997- |
| Ιατρικοί όροι (MeSH): | Helicobacter pylori*/drug effects , Helicobacter pylori*/enzymology , Helicobacter pylori*/physiology , Helicobacter pylori*/genetics , Biofilms*/drug effects , Urease*/metabolism , Anti-Bacterial Agents*/pharmacology , Antimicrobial Peptides*/pharmacology , Antimicrobial Cationic Peptides*/pharmacology, Epithelial Cells/drug effects ; Helicobacter Infections/microbiology ; Cell Membrane/drug effects ; Microbial Sensitivity Tests ; Humans |
| Περίληψη: | Aims: To investigate the in vitro antibacterial activity and related phenotypic effects of the novel antimicrobial peptide SAAP-148 against Helicobacter pylori (H. pylori). Materials and Methods: The minimum inhibitory concentration (MIC) of SAAP-148 against H. pylori strains, including multidrug-resistant isolates, was determined using the broth microdilution method. Its activity after short-term exposure to pH-adjusted conditions and its cytotoxicity toward gastric epithelial cells were evaluated. The effects of SAAP-148 on cell morphology, membrane permeability, established biofilms, bacterial viability, cell-associated urease activity, and ureA/ureB gene expression were assessed. Antibacterial activity against one four-drug-resistant H. pylori clinical isolate was further evaluated by colony counting and OD600 measurement. Results: SAAP-148 exhibited potent antibacterial activity against H. pylori strains with different resistance profiles, with MIC values ranging from 16-32 µg/mL. SAAP-148 retained antibacterial activity after short-term exposure to pH-adjusted conditions and showed low cytotoxicity toward gastric epithelial cells. SAAP-148 treatment was associated with bacterial morphological damage, increased inner and outer membrane permeability, reduced biofilm biomass and biofilm-associated protein content, decreased cell-associated urease activity, and reduced ureA/ureB mRNA expression. In the dedicated MDR model, SAAP-148 also reduced viable counts of one four-drug-resistant H. pylori clinical isolate. Conclusion: SAAP-148 demonstrated promising in vitro activity against H. pylori and was associated with membrane disruption, antibiofilm effects, and reduced urease-associated readouts. These findings support further preclinical evaluation of SAAP-148 as a potential antimicrobial strategy against difficult-to-eradicate H. pylori. (© The Author(s) 2026. Published by Oxford University Press on behalf of Applied Microbiology International. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com.) |
| Contributed Indexing: | Keywords: Helicobacter pylori; SAAP-148; antibacterial activity; antimicrobial peptide |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 0 (Anti-Bacterial Agents) 0 (Antimicrobial Peptides) 0 (Antimicrobial Cationic Peptides) |
| Entry Date(s): | Date Created: 20260613 Date Completed: 20260626 Latest Revision: 20260626 |
| Update Code: | 20260626 |
| DOI: | 10.1093/jambio/lxag145 |
| PMID: | 42287196 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1365-2672 |
|---|---|
| DOI: | 10.1093/jambio/lxag145 |