Academic Journal
Προβολή σε EDS

Genome-wide identification and temperature-responsive expression profiling of TRP gene family in red swamp crayfish (Procambarus clarkii).

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Genome-wide identification and temperature-responsive expression profiling of TRP gene family in red swamp crayfish (Procambarus clarkii).
Συγγραφείς: Yang B; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China., Ma Y; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China., Bai Z; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China., Wang Q; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China., Liu C; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China., Jia L; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China. ljia@shou.edu.cn.; Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals, Shanghai, 201306, China. ljia@shou.edu.cn., Bai Z; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China. zybai@shou.edu.cn.; Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals, Shanghai, 201306, China. zybai@shou.edu.cn.
Πηγή: BMC genomics [BMC Genomics] 2026 Jun 12; Vol. 27 (1). Date of Electronic Publication: 2026 Jun 12.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
Imprint Name(s): Original Publication: London : BioMed Central, [2000-
Ιατρικοί όροι (MeSH): Astacoidea*/genetics , Transient Receptor Potential Channels*/genetics , Transient Receptor Potential Channels*/metabolism , Gene Expression Profiling* , Multigene Family* , Temperature*, Animals ; Phylogeny ; Transcriptome ; Alternative Splicing
Περίληψη: Background: Temperature variations significantly influence physiological processes in arthropod. The transient receptor potential (TRP) channels are key cellular sensors in the detection of temperature, pain, and mechanical stimuli. However, a comprehensive genome-wide identification and functional characterization of the TRP gene family in Procambarus clarkii has not yet been conducted.
Results: In this study, we performed a genome-wide analysis and identified 29 TRP genes in P. clarkii, including 11 newly reported, which were classified into seven subfamilies: TRPA, TRPC, TRPP, TRPM, TRPML, TRPN, and TRPV. Phylogenetic analysis revealed a remarkable expansion of the TRPA subfamily in crayfish compared with insects. Tissue expression profiling demonstrated diverse transcriptional patterns of PclaTRP genes across different organs. Comparative transcriptome analysis under temperature stress further indicated that P. clarkii employs distinct molecular strategies to cope with thermal extremes: heat stress induces proteostasis disruption and biosynthetic suppression, whereas cold stress promotes controlled metabolic downregulation. Specifically, cold stress (10 °C) significantly modulated the expression of 9 PclaTRP genes (4 genes up-regulated and 5 genes down-regulated), whereas non-noxious (30 °C) and noxious heat (35 °C) challenges drastically affected the mRNA levels of 9 (6 genes up-regulated and 3 genes down-regulated) and 12 (10 genes up-regulated and 2 genes down-regulated) PclaTRP genes, respectively. Moreover, temperature-responsive alternative splicing events were identified in two key thermo-responsive candidate factors, PclaTRPA1 and PclaPain2, implicating them in thermosensation.
Conclusion: Together, these findings provide a comprehensive overview of the TRP gene family in P. clarkii, revealing their differential expression patterns and alternative splicing regulation under heat and cold challenges, and identifying candidate genes that warrant further investigation for temperature tolerance improvement in crayfish breeding.
(© 2026. The Author(s).)
Competing Interests: Declarations. Ethics approval and consent to participate: The experiment was conducted following the procedure of Animal Care and Use Committee of Shanghai Ocean University. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
References: Sci Rep. 2017 May 19;7(1):2173. (PMID: 28526851)
Nat Genet. 2005 Mar;37(3):305-10. (PMID: 15731759)
Bioinformatics. 2018 Sep 1;34(17):i884-i890. (PMID: 30423086)
Arch Environ Contam Toxicol. 2013 May;64(4):676-91. (PMID: 23329359)
Nature. 2011 Dec 04;481(7379):76-80. (PMID: 22139422)
Nature. 2024 Jun;630(8016):493-500. (PMID: 38718835)
Nature. 1997 Oct 23;389(6653):816-24. (PMID: 9349813)
Imeta. 2022 Sep 28;1(4):e56. (PMID: 38867905)
J Neurosci. 2008 Oct 1;28(40):9929-38. (PMID: 18829951)
Mol Biol Evol. 2015 Jan;32(1):268-74. (PMID: 25371430)
Genome Biol. 2011;12(3):R22. (PMID: 21410973)
Nucleic Acids Res. 2019 Jan 8;47(D1):D330-D338. (PMID: 30395331)
Chem Senses. 2016 Mar;41(3):261-72. (PMID: 26826723)
Bioinformatics. 2015 Jan 15;31(2):166-9. (PMID: 25260700)
Pflugers Arch. 2018 May;470(5):745-759. (PMID: 29484488)
Genes (Basel). 2021 Feb 21;12(2):. (PMID: 33669984)
PLoS One. 2020 Mar 12;15(3):e0230266. (PMID: 32163507)
Cell Metab. 2010 Oct 6;12(4):386-397. (PMID: 20889130)
Cell. 2002 Mar 8;108(5):705-15. (PMID: 11893340)
PLoS One. 2011;6(8):e24343. (PMID: 21909389)
Proc Natl Acad Sci U S A. 2011 Nov 15;108(46):E1184-91. (PMID: 21930928)
Life Sci. 2013 Mar 19;92(8-9):394-403. (PMID: 22877650)
Nucleic Acids Res. 2002 Jul 15;30(14):3059-66. (PMID: 12136088)
Science. 2014 May 2;344(6183):1247579. (PMID: 24786084)
Curr Biol. 2016 Dec 5;26(23):3116-3128. (PMID: 27818173)
BMC Genomics. 2018 Sep 14;19(1):674. (PMID: 30217143)
Mol Biosyst. 2008 May;4(5):372-9. (PMID: 18414734)
Cells. 2019 Dec 24;9(1):. (PMID: 31878344)
Front Physiol. 2022 Jan 31;12:827077. (PMID: 35173626)
Genome Biol. 2014;15(12):550. (PMID: 25516281)
Comp Biochem Physiol A Mol Integr Physiol. 2023 Apr;278:111357. (PMID: 36572141)
Neuron. 2015 May 6;86(3):665-71. (PMID: 25950634)
BMC Genomics. 2024 Jan 17;25(1):72. (PMID: 38233779)
Cell. 2003 Apr 18;113(2):261-73. (PMID: 12705873)
Cell Rep. 2012 Jan 26;1(1):43-55. (PMID: 22347718)
Nature. 2000 May 11;405(6783):183-7. (PMID: 10821274)
Proc Biol Sci. 2020 Aug 26;287(1933):20201309. (PMID: 32842926)
Comp Biochem Physiol B Biochem Mol Biol. 2022 Jan;257:110654. (PMID: 34371155)
J Therm Biol. 2019 Oct;85:102404. (PMID: 31657746)
Nature. 2003 Jun 19;423(6942):822-3. (PMID: 12815418)
Bioinformatics. 2009 Aug 1;25(15):1972-3. (PMID: 19505945)
Nature. 2002 Mar 7;416(6876):52-8. (PMID: 11882888)
Nucleic Acids Res. 2008 Jan;36(Database issue):D480-4. (PMID: 18077471)
Nat Methods. 2015 Apr;12(4):357-60. (PMID: 25751142)
Nature. 1975 Nov 6;258(5530):84-7. (PMID: 810728)
Cell Calcium. 2018 Jan;69:112-121. (PMID: 28689729)
Dev Comp Immunol. 2025 Sep;170:105442. (PMID: 40816337)
Proc Natl Acad Sci U S A. 2014 Dec 23;111(51):E5593-601. (PMID: 25480548)
PLoS One. 2010 May 06;5(5):e10519. (PMID: 20463899)
Neuron. 2014 Jun 4;82(5):1017-31. (PMID: 24814535)
Cell Rep. 2017 Dec 19;21(12):3329-3337. (PMID: 29262313)
Proc Natl Acad Sci U S A. 2011 Nov 1;108(44):18114-9. (PMID: 22025699)
Grant Information: 23N11900300 Shanghai Science and Technology Innovation action plan project
Contributed Indexing: Keywords: Procambarus clarkii; Alternative splicing; Gene expression; Temperature stress; Transient receptor potential channels
Substance Nomenclature: 0 (Transient Receptor Potential Channels)
Entry Date(s): Date Created: 20260613 Date Completed: 20260819 Latest Revision: 20260820
Update Code: 20260820
PubMed Central ID: PMC13483504
DOI: 10.1186/s12864-026-13020-2
PMID: 42286486
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1471-2164
DOI:10.1186/s12864-026-13020-2