Academic Journal
Melatonin alleviates mitochondrial damage by regulating SIRT3‑mediated mitochondrial dynamics in granulosa cells under PCOS‑like conditions.
| Τίτλος: | Melatonin alleviates mitochondrial damage by regulating SIRT3‑mediated mitochondrial dynamics in granulosa cells under PCOS‑like conditions. |
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| Συγγραφείς: | Xie C; Hebei Key Laboratory of Reproductive Medicine, Hebei Reproductive Health Hospital, Shijiazhuang, Hebei 050071, P.R. China., Liu Z; Institute of Paediatrics, Hebei Provincial Clinical Research Centre for Child Health and Disease, Hebei Children's Hospital, Shijiazhuang, Hebei 050031, P.R. China., Chen T; Department of Biochemistry and Molecule Biology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China., Lu H; Hebei Key Laboratory of Reproductive Medicine, Hebei Reproductive Health Hospital, Shijiazhuang, Hebei 050071, P.R. China., Zhang X; Hebei Key Laboratory of Reproductive Medicine, Hebei Reproductive Health Hospital, Shijiazhuang, Hebei 050071, P.R. China., Yu W; Hebei Key Laboratory of Reproductive Medicine, Hebei Reproductive Health Hospital, Shijiazhuang, Hebei 050071, P.R. China., Zhang J; Hebei Key Laboratory of Reproductive Medicine, Hebei Reproductive Health Hospital, Shijiazhuang, Hebei 050071, P.R. China., Shang D; Department of Biochemistry and Molecule Biology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China., Wang X; Hebei Key Laboratory of Reproductive Medicine, Hebei Reproductive Health Hospital, Shijiazhuang, Hebei 050071, P.R. China. |
| Πηγή: | Molecular medicine reports [Mol Med Rep] 2026 Aug; Vol. 34 (2). Date of Electronic Publication: 2026 Jun 11. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: D. A. Spandidos Country of Publication: Greece NLM ID: 101475259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1791-3004 (Electronic) Linking ISSN: 17912997 NLM ISO Abbreviation: Mol Med Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Athens, Greece : D. A. Spandidos |
| Ιατρικοί όροι (MeSH): | Sirtuin 3*/metabolism , Sirtuin 3*/genetics , Melatonin*/pharmacology , Polycystic Ovary Syndrome*/metabolism , Polycystic Ovary Syndrome*/pathology , Polycystic Ovary Syndrome*/drug therapy , Granulosa Cells*/metabolism , Granulosa Cells*/drug effects , Granulosa Cells*/pathology , Mitochondrial Dynamics*/drug effects , Mitochondria*/metabolism , Mitochondria*/drug effects , Mitochondria*/pathology, Mitophagy/drug effects ; Membrane Potential, Mitochondrial/drug effects ; Dihydrotestosterone/pharmacology ; Autophagy/drug effects ; Female ; Humans ; Cell Line |
| Περίληψη: | The present study investigated the role of melatonin (MT) in regulating mitochondrial function via sirtuin 3 (SIRT3) in granulosa cells (GCs) from patients with polycystic ovary syndrome (PCOS), with a focus on mitochondrial protection. Notably, GCs isolated from patients with PCOS exhibited mitochondrial dysfunction. Using an in vitro PCOS model established by treating KGN cells with dihydrotestosterone (DHT), decreased SIRT3 expression, dysregulated mitochondrial dynamics and hyperactivation of mitophagy were observed. Both SIRT3 overexpression and MT treatment restored the mitochondrial membrane potential, rebalanced mitochondrial dynamics and suppressed excessive autophagy in DHT‑treated cells. Additionally, MT levels were shown to be reduced in the follicular fluid of patients with PCOS. Notably, the protective effects of MT on proteins associated with both mitochondrial dynamics and autophagy were abolished upon SIRT3 inhibition. In conclusion, mitochondrial dysfunction and aberrant mitophagy in GCs may serve a role in the pathogenesis of PCOS. MT appears to ameliorate these defects by modulating mitochondrial dynamics and function in a SIRT3‑dependent manner. Moreover, the current study identified SIRT3 as a key molecular target of MT in PCOS. |
| Contributed Indexing: | Keywords: melatonin; mitochondrial function; polycystic ovary syndrome; sirtuin 3 |
| Substance Nomenclature: | EC 3.5.1.- (Sirtuin 3) JL5DK93RCL (Melatonin) EC 3.5.1.- (SIRT3 protein, human) 08J2K08A3Y (Dihydrotestosterone) |
| Entry Date(s): | Date Created: 20260611 Date Completed: 20260613 Latest Revision: 20260726 |
| Update Code: | 20260726 |
| PubMed Central ID: | PMC13280408 |
| DOI: | 10.3892/mmr.2026.13937 |
| PMID: | 42272252 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1791-3004 |
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| DOI: | 10.3892/mmr.2026.13937 |