Academic Journal
Development of a rapid and sensitive EnLIGHT OMEGA assay for extracellular AGR2 detection in biological fluids.
| Τίτλος: | Development of a rapid and sensitive EnLIGHT OMEGA assay for extracellular AGR2 detection in biological fluids. |
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| Συγγραφείς: | Gosset L; OncoGyne Lab, Univ. Bordeaux, INSERM U1312, F-33000 Bordeaux, France., Babin G; OncoGyne Lab, Univ. Bordeaux, INSERM U1312, F-33000 Bordeaux, France; Institut Bergonié, Bordeaux, France., Roby P; BioSignal(2) Inc, 2271 boul. Fernand-Lafontaine Suite 010, Longueuil, Québec, J4G 2R7, Canada., Fafard C; BioSignal(2) Inc, 2271 boul. Fernand-Lafontaine Suite 010, Longueuil, Québec, J4G 2R7, Canada., Bosse R; BioSignal(2) Inc, 2271 boul. Fernand-Lafontaine Suite 010, Longueuil, Québec, J4G 2R7, Canada. Electronic address: roger.bosse@biosignal2.com., Delom F; OncoGyne Lab, Univ. Bordeaux, INSERM U1312, F-33000 Bordeaux, France. Electronic address: frederic.delom@u-bordeaux.fr., Fessart D; OncoGyne Lab, Univ. Bordeaux, INSERM U1312, F-33000 Bordeaux, France. Electronic address: delphine.fessart@inserm.fr. |
| Πηγή: | SLAS discovery : advancing life sciences R & D [SLAS Discov] 2026 Sep; Vol. 42, pp. 100315. Date of Electronic Publication: 2026 Jun 10. |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: SAGE Publications Country of Publication: United States NLM ID: 101697563 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2472-5560 (Electronic) Linking ISSN: 24725552 NLM ISO Abbreviation: SLAS Discov Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Thousand Oaks, CA : SAGE Publications, [2017]- |
| Ιατρικοί όροι (MeSH): | Mucoproteins*/metabolism , Mucoproteins*/analysis , Mucoproteins*/blood , Oncogene Proteins*/metabolism , Body Fluids*/metabolism , Body Fluids*/chemistry, Fluorescence Resonance Energy Transfer/methods ; Humans |
| Περίληψη: | Anterior gradient 2 (AGR2) is an endoplasmic reticulum -resident protein belonging to the disulfide isomerase family. AGR2 contributes to protein quality control and unfolded protein response (UPR). In addition to its canonical intracellular role, AGR2 also exists in an extracellular form (eAGR2), which is strongly associated with tumour progression and cancer aggressiveness. The objective was to develop a novel approach for identifying and quantifying AGR2 from a broad range of biological fluids (cell lysates, extracellular medium, serum). Therefore, an amplified luminescent proximity homogeneous assay (EnLIGHT OMEGA ™) was established using a proximity method based on the principles of fluorescence resonance energy transfer and antigen-antibody specific binding. This method is sensitive, rapid, performed without washing, and can detect 0.02-300 ng/mL of AGR2. In conclusion, our study establishes a simple, sensitive, and rapid method for eAGR2 detection in oncology applications, including cancer drug resistance and metastatic progression. (Copyright © 2026 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of competing interest LG, DF, FD, GB declare no conflict of interests. PR, CF and RB are employees of BioSignal2 Inc. |
| Contributed Indexing: | Keywords: AGR2; Biological fluids; EnLIGHT OMEGA; Quantification |
| Substance Nomenclature: | 0 (Mucoproteins) 0 (Oncogene Proteins) 0 (AGR2 protein, human) |
| Entry Date(s): | Date Created: 20260610 Date Completed: 20260707 Latest Revision: 20260709 |
| Update Code: | 20260711 |
| DOI: | 10.1016/j.slasd.2026.100315 |
| PMID: | 42269775 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2472-5560 |
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| DOI: | 10.1016/j.slasd.2026.100315 |