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FLIM Playground: An interactive, end-to-end graphical user interface for analyzing single cells with fluorescence lifetime imaging microscopy.

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Τίτλος: FLIM Playground: An interactive, end-to-end graphical user interface for analyzing single cells with fluorescence lifetime imaging microscopy.
Συγγραφείς: Zhao W; Morgridge Institute for Research, Madison, WI, USA., Samimi K; Morgridge Institute for Research, Madison, WI, USA., Skala MC; Morgridge Institute for Research, Madison, WI, USA; Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA., Datta R; Morgridge Institute for Research, Madison, WI, USA. Electronic address: rdatta@morgridge.org.
Πηγή: Cell reports methods [Cell Rep Methods] 2026 Aug 17; Vol. 6 (8), pp. 101484. Date of Electronic Publication: 2026 Jun 10.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Elsevier Inc Country of Publication: United States NLM ID: 9918227360606676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2667-2375 (Electronic) Linking ISSN: 26672375 NLM ISO Abbreviation: Cell Rep Methods Subsets: MEDLINE
Imprint Name(s): Original Publication: [New York] : Elsevier Inc., [2021]-
Ιατρικοί όροι (MeSH): Single-Cell Analysis*/methods , User-Computer Interface*, Microscopy, Fluorescence/methods ; Image Processing, Computer-Assisted/methods ; Humans ; Software ; Cell Line, Tumor
Περίληψη: Fluorescence lifetime imaging microscopy (FLIM) is sensitive to molecular environments and enables high-resolution mapping of cellular heterogeneity. Yet, the journey from raw photon decays to biological insight remains fragmented by multi-step data extraction and siloed analyses, creating burdens for experts and non-experts alike. This work presents FLIM Playground, the first interactive graphical platform that unifies single-cell FLIM workflows. Modularly designed to encompass data extraction (if desired) and data analysis, FLIM Playground can check field-of-view metadata, calibrate and extract fluorescence lifetime features per region of interest along with morphology and texture features across channels, merge multiple datasets, and provide real-time visual analytic modules. Lifetime extraction was validated against commercial software and published results, and both data extraction and analysis were demonstrated on a FLIM dataset of cancer cell lines. By adopting best practices and offering interactivity, FLIM Playground promotes reproducibility, allows for expansion to new imaging modalities, and accelerates hypothesis-driven discovery.
(Copyright © 2026 The Authors. Published by Elsevier Inc. All rights reserved.)
Competing Interests: Declaration of interests M.C.S. is an adviser to Elephas Biosciences. All other authors declare they have no competing interests.
Σχόλια: Update of: bioRxiv. 2025 Nov 17:2025.09.30.679625. doi: 10.1101/2025.09.30.679625.. (PMID: 41256698)
References: Front Oncol. 2020 May 15;10:553. (PMID: 32500020)
PLoS One. 2013 Aug 05;8(8):e70687. (PMID: 23940626)
PLoS One. 2020 Dec 30;15(12):e0238327. (PMID: 33378370)
Biophys J. 2008 Jan 15;94(2):L14-6. (PMID: 17981902)
Nature. 2020 Sep;585(7825):357-362. (PMID: 32939066)
BMC Bioinformatics. 2021 Sep 10;22(1):433. (PMID: 34507520)
Methods Cell Biol. 2007;81:495-524. (PMID: 17519182)
Biometrika. 1947;34(1-2):28-35. (PMID: 20287819)
Sci Rep. 2025 Feb 14;15(1):5548. (PMID: 39952935)
Nat Methods. 2025 Mar;22(3):592-599. (PMID: 39939718)
Nat Protoc. 2018 Sep;13(9):1979-2004. (PMID: 30190551)
Biol Imaging. 2023 Nov 06;3:e21. (PMID: 38487690)
Front Immunol. 2025 Aug 07;16:1617993. (PMID: 40852713)
Nat Methods. 2020 Mar;17(3):261-272. (PMID: 32015543)
J Biomed Opt. 2026 Jul;31(7):076501. (PMID: 42394640)
J Biomed Opt. 2025 Dec;29(Suppl 2):S22702. (PMID: 38434231)
Cytometry A. 2024 Aug;105(8):607-620. (PMID: 38943226)
Proc Natl Acad Sci U S A. 2022 Mar 1;119(9):. (PMID: 35217616)
Trends Cell Biol. 1999 Feb;9(2):48-52. (PMID: 10087617)
J Biomed Opt. 2020 May;25(7):1-43. (PMID: 32406215)
Anal Chem. 2025 Jun 10;97(22):11382-11387. (PMID: 40408562)
Grant Information: R01 CA272855 United States CA NCI NIH HHS; R01 CA278051 United States CA NCI NIH HHS; R01 HL165726 United States HL NHLBI NIH HHS; R37 CA226526 United States CA NCI NIH HHS
Contributed Indexing: Keywords: CP: imaging; FLIM; GUI; TCSPC; fluorescence lifetime imaging microscopy; image analysis; interactive visualization; lifetime fitting; metabolic imaging; open-source software; phasor analysis
Entry Date(s): Date Created: 20260610 Date Completed: 20260817 Latest Revision: 20260824
Update Code: 20260824
PubMed Central ID: PMC13494553
DOI: 10.1016/j.crmeth.2026.101484
PMID: 42269617
Βάση Δεδομένων: MEDLINE
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  Data: FLIM Playground: An interactive, end-to-end graphical user interface for analyzing single cells with fluorescence lifetime imaging microscopy.
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  Data: <searchLink fieldCode="AU" term="%22Zhao+W%22">Zhao W</searchLink>; Morgridge Institute for Research, Madison, WI, USA.<br /><searchLink fieldCode="AU" term="%22Samimi+K%22">Samimi K</searchLink>; Morgridge Institute for Research, Madison, WI, USA.<br /><searchLink fieldCode="AU" term="%22Skala+MC%22">Skala MC</searchLink>; Morgridge Institute for Research, Madison, WI, USA; Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA.<br /><searchLink fieldCode="AU" term="%22Datta+R%22">Datta R</searchLink>; Morgridge Institute for Research, Madison, WI, USA. Electronic address: rdatta@morgridge.org.
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  Data: <searchLink fieldCode="JN" term="%229918227360606676%22">Cell reports methods</searchLink> [Cell Rep Methods] 2026 Aug 17; Vol. 6 (8), pp. 101484. <i>Date of Electronic Publication: </i>2026 Jun 10.
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  Data: Fluorescence lifetime imaging microscopy (FLIM) is sensitive to molecular environments and enables high-resolution mapping of cellular heterogeneity. Yet, the journey from raw photon decays to biological insight remains fragmented by multi-step data extraction and siloed analyses, creating burdens for experts and non-experts alike. This work presents FLIM Playground, the first interactive graphical platform that unifies single-cell FLIM workflows. Modularly designed to encompass data extraction (if desired) and data analysis, FLIM Playground can check field-of-view metadata, calibrate and extract fluorescence lifetime features per region of interest along with morphology and texture features across channels, merge multiple datasets, and provide real-time visual analytic modules. Lifetime extraction was validated against commercial software and published results, and both data extraction and analysis were demonstrated on a FLIM dataset of cancer cell lines. By adopting best practices and offering interactivity, FLIM Playground promotes reproducibility, allows for expansion to new imaging modalities, and accelerates hypothesis-driven discovery.<br /> (Copyright © 2026 The Authors. Published by Elsevier Inc. All rights reserved.)
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  Data: Declaration of interests M.C.S. is an adviser to Elephas Biosciences. All other authors declare they have no competing interests.
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  Data: Update of: bioRxiv. 2025 Nov 17:2025.09.30.679625. doi: 10.1101/2025.09.30.679625.. (PMID: <searchLink fieldCode="PM" term="%2241256698%22">41256698)</searchLink>
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  Data: Front Oncol. 2020 May 15;10:553. (PMID: <searchLink fieldCode="PM" term="%2232500020%22">32500020)</searchLink><br />PLoS One. 2013 Aug 05;8(8):e70687. (PMID: <searchLink fieldCode="PM" term="%2223940626%22">23940626)</searchLink><br />PLoS One. 2020 Dec 30;15(12):e0238327. (PMID: <searchLink fieldCode="PM" term="%2233378370%22">33378370)</searchLink><br />Biophys J. 2008 Jan 15;94(2):L14-6. (PMID: <searchLink fieldCode="PM" term="%2217981902%22">17981902)</searchLink><br />Nature. 2020 Sep;585(7825):357-362. (PMID: <searchLink fieldCode="PM" term="%2232939066%22">32939066)</searchLink><br />BMC Bioinformatics. 2021 Sep 10;22(1):433. (PMID: <searchLink fieldCode="PM" term="%2234507520%22">34507520)</searchLink><br />Methods Cell Biol. 2007;81:495-524. (PMID: <searchLink fieldCode="PM" term="%2217519182%22">17519182)</searchLink><br />Biometrika. 1947;34(1-2):28-35. (PMID: <searchLink fieldCode="PM" term="%2220287819%22">20287819)</searchLink><br />Sci Rep. 2025 Feb 14;15(1):5548. (PMID: <searchLink fieldCode="PM" term="%2239952935%22">39952935)</searchLink><br />Nat Methods. 2025 Mar;22(3):592-599. (PMID: <searchLink fieldCode="PM" term="%2239939718%22">39939718)</searchLink><br />Nat Protoc. 2018 Sep;13(9):1979-2004. (PMID: <searchLink fieldCode="PM" term="%2230190551%22">30190551)</searchLink><br />Biol Imaging. 2023 Nov 06;3:e21. (PMID: <searchLink fieldCode="PM" term="%2238487690%22">38487690)</searchLink><br />Front Immunol. 2025 Aug 07;16:1617993. (PMID: <searchLink fieldCode="PM" term="%2240852713%22">40852713)</searchLink><br />Nat Methods. 2020 Mar;17(3):261-272. (PMID: <searchLink fieldCode="PM" term="%2232015543%22">32015543)</searchLink><br />J Biomed Opt. 2026 Jul;31(7):076501. (PMID: <searchLink fieldCode="PM" term="%2242394640%22">42394640)</searchLink><br />J Biomed Opt. 2025 Dec;29(Suppl 2):S22702. (PMID: <searchLink fieldCode="PM" term="%2238434231%22">38434231)</searchLink><br />Cytometry A. 2024 Aug;105(8):607-620. (PMID: <searchLink fieldCode="PM" term="%2238943226%22">38943226)</searchLink><br />Proc Natl Acad Sci U S A. 2022 Mar 1;119(9):. (PMID: <searchLink fieldCode="PM" term="%2235217616%22">35217616)</searchLink><br />Trends Cell Biol. 1999 Feb;9(2):48-52. (PMID: <searchLink fieldCode="PM" term="%2210087617%22">10087617)</searchLink><br />J Biomed Opt. 2020 May;25(7):1-43. (PMID: <searchLink fieldCode="PM" term="%2232406215%22">32406215)</searchLink><br />Anal Chem. 2025 Jun 10;97(22):11382-11387. (PMID: <searchLink fieldCode="PM" term="%2240408562%22">40408562)</searchLink>
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