Academic Journal
FLIM Playground: An interactive, end-to-end graphical user interface for analyzing single cells with fluorescence lifetime imaging microscopy.
| Τίτλος: | FLIM Playground: An interactive, end-to-end graphical user interface for analyzing single cells with fluorescence lifetime imaging microscopy. |
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| Συγγραφείς: | Zhao W; Morgridge Institute for Research, Madison, WI, USA., Samimi K; Morgridge Institute for Research, Madison, WI, USA., Skala MC; Morgridge Institute for Research, Madison, WI, USA; Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA., Datta R; Morgridge Institute for Research, Madison, WI, USA. Electronic address: rdatta@morgridge.org. |
| Πηγή: | Cell reports methods [Cell Rep Methods] 2026 Aug 17; Vol. 6 (8), pp. 101484. Date of Electronic Publication: 2026 Jun 10. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Inc Country of Publication: United States NLM ID: 9918227360606676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2667-2375 (Electronic) Linking ISSN: 26672375 NLM ISO Abbreviation: Cell Rep Methods Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: [New York] : Elsevier Inc., [2021]- |
| Ιατρικοί όροι (MeSH): | Single-Cell Analysis*/methods , User-Computer Interface*, Microscopy, Fluorescence/methods ; Image Processing, Computer-Assisted/methods ; Humans ; Software ; Cell Line, Tumor |
| Περίληψη: | Fluorescence lifetime imaging microscopy (FLIM) is sensitive to molecular environments and enables high-resolution mapping of cellular heterogeneity. Yet, the journey from raw photon decays to biological insight remains fragmented by multi-step data extraction and siloed analyses, creating burdens for experts and non-experts alike. This work presents FLIM Playground, the first interactive graphical platform that unifies single-cell FLIM workflows. Modularly designed to encompass data extraction (if desired) and data analysis, FLIM Playground can check field-of-view metadata, calibrate and extract fluorescence lifetime features per region of interest along with morphology and texture features across channels, merge multiple datasets, and provide real-time visual analytic modules. Lifetime extraction was validated against commercial software and published results, and both data extraction and analysis were demonstrated on a FLIM dataset of cancer cell lines. By adopting best practices and offering interactivity, FLIM Playground promotes reproducibility, allows for expansion to new imaging modalities, and accelerates hypothesis-driven discovery. (Copyright © 2026 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of interests M.C.S. is an adviser to Elephas Biosciences. All other authors declare they have no competing interests. |
| Σχόλια: | Update of: bioRxiv. 2025 Nov 17:2025.09.30.679625. doi: 10.1101/2025.09.30.679625.. (PMID: 41256698) |
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| Grant Information: | R01 CA272855 United States CA NCI NIH HHS; R01 CA278051 United States CA NCI NIH HHS; R01 HL165726 United States HL NHLBI NIH HHS; R37 CA226526 United States CA NCI NIH HHS |
| Contributed Indexing: | Keywords: CP: imaging; FLIM; GUI; TCSPC; fluorescence lifetime imaging microscopy; image analysis; interactive visualization; lifetime fitting; metabolic imaging; open-source software; phasor analysis |
| Entry Date(s): | Date Created: 20260610 Date Completed: 20260817 Latest Revision: 20260824 |
| Update Code: | 20260824 |
| PubMed Central ID: | PMC13494553 |
| DOI: | 10.1016/j.crmeth.2026.101484 |
| PMID: | 42269617 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2667-2375 |
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| DOI: | 10.1016/j.crmeth.2026.101484 |