Academic Journal
Dialysis bioreactor enables high-density, serum-free expansion of dental pulp-derived mesenchymal stromal cells with enhanced secretome.
| Τίτλος: | Dialysis bioreactor enables high-density, serum-free expansion of dental pulp-derived mesenchymal stromal cells with enhanced secretome. |
|---|---|
| Συγγραφείς: | Torizal FG; Biotechnology Product Development Laboratory, Department of Biotechnology, Faculty of Science and Technology, Universitas Aisyiyah Yogyakarta, Yogyakarta, Indonesia; Division of Stem Cell Biomanufacturing, Research Group for Regenerative Application of Functional Tissues (GRAFT), Yogyakarta, Indonesia. Electronic address: t_gandhi@unisayogya.ac.id., Tirtadani Y; Biotechnology Product Development Laboratory, Department of Biotechnology, Faculty of Science and Technology, Universitas Aisyiyah Yogyakarta, Yogyakarta, Indonesia; Division of Stem Cell Biomanufacturing, Research Group for Regenerative Application of Functional Tissues (GRAFT), Yogyakarta, Indonesia., Gunady AV; Department of Biotechnology, Graduate School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, Indonesia., Mohamed EMA; Biotechnology Product Development Laboratory, Department of Biotechnology, Faculty of Science and Technology, Universitas Aisyiyah Yogyakarta, Yogyakarta, Indonesia; Division of Stem Cell Biomanufacturing, Research Group for Regenerative Application of Functional Tissues (GRAFT), Yogyakarta, Indonesia., Angelius C; Faculty of Medicine, Universitas Pelita Harapan, Tangerang, Banten, Indonesia. |
| Πηγή: | Cytotherapy [Cytotherapy] 2026 Aug; Vol. 28 (8), pp. 102820. Date of Electronic Publication: 2026 Apr 04. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: England NLM ID: 100895309 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-2566 (Electronic) Linking ISSN: 14653249 NLM ISO Abbreviation: Cytotherapy Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2013- : London : Elsevier Original Publication: Oxford, England : ISIS Medical Media, c1999- |
| Ιατρικοί όροι (MeSH): | Mesenchymal Stem Cells*/cytology , Mesenchymal Stem Cells*/metabolism , Dental Pulp*/cytology , Secretome*/metabolism , Cell Culture Techniques*/methods , Bioreactors*, Humans ; Culture Media, Serum-Free ; Cell Differentiation ; Cell Proliferation ; Dialysis ; Cells, Cultured |
| Περίληψη: | Dental pulp-derived mesenchymal stromal cells (DPSCs) offer a potential source for both autologous cell-based and cell-free therapies aimed at treating a wide range of diseases. However, current culture practices predominantly rely on serum-based media, which introduce variability and contain potential xenogeneic components, limiting their applicability for therapeutic use. While serum-free media containing growth factors offer an alternative solution to mitigate this issue, they are often inefficient and costly due to the removal of remaining essential growth factors during media replacement. In this study, we developed a scalable dialysis bioreactor that recycles and accumulates endogenous and exogenous growth factors, allowing for the efficient high-density propagation of DPSCs. This bioreactor achieved a cell density of approximately 4.1 × 10⁷ cells/mL DPSCs per batch in a serum-free environment, while maintaining multipotency and differentiation potential after long-term culture. DPSCs grown with this system demonstrated improved proliferation, preserved stemness and enhanced differentiation capacity into odontogenic, osteogenic, chondrogenic, adipogenic and neurogenic lineages. Additionally, the profile of secreted therapeutic proteins related to immunomodulation and tissue regeneration significantly improved compared to manual medium replacement method. By making optimal use of similar amounts of growth factors, the production efficiency significantly increased relative to traditional serum-free culture system. In summary, the dialysis-based bioreactor offers a proof of concept for a scalable platform to improve DPSCs production and optimize their secreted products for therapeutic use. This system overcomes key challenges in serum-free DPSC culture by providing a scalable, efficient and clinically relevant biomanufacturing process. (Copyright © 2026 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of Competing Interest The submission is original, has not been previously published and is not currently under consideration elsewhere. We declare that there are no conflicts of interest. All authors have approved the manuscript. |
| Contributed Indexing: | Keywords: DPSCs; dialysis bioreactor; growth factor; high cell density; serum-free media |
| Substance Nomenclature: | 0 (Culture Media, Serum-Free) |
| Entry Date(s): | Date Created: 20260610 Date Completed: 20260707 Latest Revision: 20260707 |
| Update Code: | 20260708 |
| DOI: | 10.1016/j.jcyt.2026.102820 |
| PMID: | 42269277 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1477-2566 |
|---|---|
| DOI: | 10.1016/j.jcyt.2026.102820 |