Academic Journal
Προβολή σε EDS

Epitope Mapping of Anti-Mouse C-C Motif Chemokine Receptor 1 Monoclonal Antibodies Developed by the Cell-Based Immunization and Screening Method.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Epitope Mapping of Anti-Mouse C-C Motif Chemokine Receptor 1 Monoclonal Antibodies Developed by the Cell-Based Immunization and Screening Method.
Συγγραφείς: Okada A; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan., Suzuki H; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan., Kaneko MK; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan., Kato Y; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
Πηγή: Monoclonal antibodies in immunodiagnosis and immunotherapy [Monoclon Antib Immunodiagn Immunother] 2026 Jul-Aug; Vol. 45 (4), pp. 58-68. Date of Electronic Publication: 2026 Jun 10.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101590955 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2167-9436 (Electronic) Linking ISSN: 21679436 NLM ISO Abbreviation: Monoclon Antib Immunodiagn Immunother Subsets: MEDLINE
Imprint Name(s): Original Publication: New Rochelle, NY : Mary Ann Liebert, Inc.
Ιατρικοί όροι (MeSH): Epitope Mapping*/methods , Antibodies, Monoclonal*/immunology , Receptors, CCR1*/immunology , Receptors, CCR1*/genetics , Epitopes*/immunology , Epitopes*/chemistry, Animals ; Mice ; Flow Cytometry ; Immunization ; Humans
Περίληψη: The C-C motif chemokine receptor 1 (CCR1) plays key roles in guiding leukocyte movement during immune surveillance and inflammatory responses. Targeting CCR1 is a promising approach for treating autoimmune diseases and cancers. We previously developed monoclonal antibodies against mouse CCR1 (mCCR1) (clones C1Mab-2 and C1Mab-6) for use in flow cytometry and Western blotting. However, the specific binding sites have not yet been identified. This study examined the binding epitope of C1Mab-2 and C1Mab-6 using flow cytometry. Analysis of mCCR1 mutants with altered extracellular domains showed that C1Mab-2 and C1Mab-6 bind to the N-terminal region of mCCR1. Additionally, PA-tag substitution experiments identified the epitope as 1st Met and amino acids 2-13 of mCCR1. Further alanine (or glycine) scanning within the N-terminal region (amino acids 2-13) demonstrated that Glu2, Asp5, and Phe6 are essential for recognition by C1Mab-2, and Glu2, Ile3, and Asp5 are crucial for recognition by C1Mab-6 in flow cytometry and Western blotting. These findings contribute to the understanding of mCCR1 and C1Mab interaction.
Competing Interests: Author Disclosure StatementThe authors have no conflicts of interest.
Contributed Indexing: Keywords: alanine scanning; epitope mapping; flow cytometry; monoclonal antibody; mouse CCR1
Substance Nomenclature: 0 (Antibodies, Monoclonal)
0 (Receptors, CCR1)
0 (Epitopes)
0 (Ccr1 protein, mouse)
Entry Date(s): Date Created: 20260610 Date Completed: 20260702 Latest Revision: 20260702
Update Code: 20260703
DOI: 10.1177/21679436261459606
PMID: 42267894
Βάση Δεδομένων: MEDLINE
FullText Text:
  Availability: 0
Header DbId: cmedm
DbLabel: MEDLINE
An: 42267894
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Epitope Mapping of Anti-Mouse C-C Motif Chemokine Receptor 1 Monoclonal Antibodies Developed by the Cell-Based Immunization and Screening Method.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Okada+A%22">Okada A</searchLink>; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.<br /><searchLink fieldCode="AU" term="%22Suzuki+H%22">Suzuki H</searchLink>; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.<br /><searchLink fieldCode="AU" term="%22Kaneko+MK%22">Kaneko MK</searchLink>; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.<br /><searchLink fieldCode="AU" term="%22Kato+Y%22">Kato Y</searchLink>; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22101590955%22">Monoclonal antibodies in immunodiagnosis and immunotherapy</searchLink> [Monoclon Antib Immunodiagn Immunother] 2026 Jul-Aug; Vol. 45 (4), pp. 58-68. <i>Date of Electronic Publication: </i>2026 Jun 10.
– Name: TypePub
  Label: Publication Type
  Group: TypPub
  Data: Journal Article
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Mary+Ann+Liebert%2C+Inc%22">Mary Ann Liebert, Inc </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>101590955 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>2167-9436 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2221679436%22">21679436 </searchLink><i>NLM ISO Abbreviation: </i>Monoclon Antib Immunodiagn Immunother <i>Subsets: </i>MEDLINE
– Name: PublisherInfo
  Label: Imprint Name(s)
  Group: PubInfo
  Data: <i>Original Publication</i>: New Rochelle, NY : Mary Ann Liebert, Inc.
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Epitope+Mapping%22">Epitope Mapping*</searchLink>/<searchLink fieldCode="MM" term="%22Epitope+Mapping+methods%22">methods</searchLink> <br /><searchLink fieldCode="MM" term="%22Antibodies%2C+Monoclonal%22">Antibodies, Monoclonal*</searchLink>/<searchLink fieldCode="MM" term="%22Antibodies%2C+Monoclonal+immunology%22">immunology</searchLink> <br /><searchLink fieldCode="MM" term="%22Receptors%2C+CCR1%22">Receptors, CCR1*</searchLink>/<searchLink fieldCode="MM" term="%22Receptors%2C+CCR1+immunology%22">immunology</searchLink> <br /><searchLink fieldCode="MM" term="%22Receptors%2C+CCR1%22">Receptors, CCR1*</searchLink>/<searchLink fieldCode="MM" term="%22Receptors%2C+CCR1+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Epitopes%22">Epitopes*</searchLink>/<searchLink fieldCode="MM" term="%22Epitopes+immunology%22">immunology</searchLink> <br /><searchLink fieldCode="MM" term="%22Epitopes%22">Epitopes*</searchLink>/<searchLink fieldCode="MM" term="%22Epitopes+chemistry%22">chemistry</searchLink><br /><searchLink fieldCode="MH" term="%22Animals%22">Animals</searchLink> ; <searchLink fieldCode="MH" term="%22Mice%22">Mice</searchLink> ; <searchLink fieldCode="MH" term="%22Flow+Cytometry%22">Flow Cytometry</searchLink> ; <searchLink fieldCode="MH" term="%22Immunization%22">Immunization</searchLink> ; <searchLink fieldCode="MH" term="%22Humans%22">Humans</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The C-C motif chemokine receptor 1 (CCR1) plays key roles in guiding leukocyte movement during immune surveillance and inflammatory responses. Targeting CCR1 is a promising approach for treating autoimmune diseases and cancers. We previously developed monoclonal antibodies against mouse CCR1 (mCCR1) (clones C<subscript>1</subscript>Mab-2 and C<subscript>1</subscript>Mab-6) for use in flow cytometry and Western blotting. However, the specific binding sites have not yet been identified. This study examined the binding epitope of C<subscript>1</subscript>Mab-2 and C<subscript>1</subscript>Mab-6 using flow cytometry. Analysis of mCCR1 mutants with altered extracellular domains showed that C<subscript>1</subscript>Mab-2 and C<subscript>1</subscript>Mab-6 bind to the N-terminal region of mCCR1. Additionally, PA-tag substitution experiments identified the epitope as 1st Met and amino acids 2-13 of mCCR1. Further alanine (or glycine) scanning within the N-terminal region (amino acids 2-13) demonstrated that Glu2, Asp5, and Phe6 are essential for recognition by C<subscript>1</subscript>Mab-2, and Glu2, Ile3, and Asp5 are crucial for recognition by C<subscript>1</subscript>Mab-6 in flow cytometry and Western blotting. These findings contribute to the understanding of mCCR1 and C<subscript>1</subscript>Mab interaction.
– Name: Abstract
  Label: Competing Interests
  Group: Ab
  Data: Author Disclosure StatementThe authors have no conflicts of interest.
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>alanine scanning; epitope mapping; flow cytometry; monoclonal antibody; mouse CCR1
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 0 (Antibodies, Monoclonal)<br />0 (Receptors, CCR1)<br />0 (Epitopes)<br />0 (Ccr1 protein, mouse)
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: <i>Date Created: </i>20260610 <i>Date Completed: </i>20260702 <i>Latest Revision: </i>20260702
– Name: DateUpdate
  Label: Update Code
  Group: Date
  Data: 20260703
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1177/21679436261459606
– Name: AN
  Label: PMID
  Group: ID
  Data: 42267894
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42267894
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1177/21679436261459606
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: 58
    Subjects:
      – SubjectFull: Animals
        Type: general
      – SubjectFull: Mice
        Type: general
      – SubjectFull: Flow Cytometry
        Type: general
      – SubjectFull: Immunization
        Type: general
      – SubjectFull: Humans
        Type: general
      – SubjectFull: Epitope Mapping methods
        Type: general
      – SubjectFull: Antibodies, Monoclonal immunology
        Type: general
      – SubjectFull: Receptors, CCR1 immunology
        Type: general
      – SubjectFull: Receptors, CCR1 genetics
        Type: general
      – SubjectFull: Epitopes immunology
        Type: general
      – SubjectFull: Epitopes chemistry
        Type: general
    Titles:
      – TitleFull: Epitope Mapping of Anti-Mouse C-C Motif Chemokine Receptor 1 Monoclonal Antibodies Developed by the Cell-Based Immunization and Screening Method.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Okada A
      – PersonEntity:
          Name:
            NameFull: Suzuki H
      – PersonEntity:
          Name:
            NameFull: Kaneko MK
      – PersonEntity:
          Name:
            NameFull: Kato Y
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 07
              Text: 2026 Jul-Aug
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-electronic
              Value: 2167-9436
          Numbering:
            – Type: volume
              Value: 45
            – Type: issue
              Value: 4
          Titles:
            – TitleFull: Monoclonal antibodies in immunodiagnosis and immunotherapy
              Type: main
ResultId 1