Epitope Mapping of Anti-Mouse C-C Motif Chemokine Receptor 1 Monoclonal Antibodies Developed by the Cell-Based Immunization and Screening Method.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Epitope Mapping of Anti-Mouse C-C Motif Chemokine Receptor 1 Monoclonal Antibodies Developed by the Cell-Based Immunization and Screening Method.
Συγγραφείς:	Okada A; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan., Suzuki H; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan., Kaneko MK; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan., Kato Y; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
Πηγή:	Monoclonal antibodies in immunodiagnosis and immunotherapy [Monoclon Antib Immunodiagn Immunother] 2026 Jul-Aug; Vol. 45 (4), pp. 58-68. Date of Electronic Publication: 2026 Jun 10.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101590955 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2167-9436 (Electronic) Linking ISSN: 21679436 NLM ISO Abbreviation: Monoclon Antib Immunodiagn Immunother Subsets: MEDLINE
Imprint Name(s):	Original Publication: New Rochelle, NY : Mary Ann Liebert, Inc.
Ιατρικοί όροι (MeSH):	Epitope Mapping/methods , Antibodies, Monoclonal/immunology , Receptors, CCR1/immunology , Receptors, CCR1/genetics , Epitopes/immunology , Epitopes/chemistry, Animals ; Mice ; Flow Cytometry ; Immunization ; Humans
Περίληψη:	The C-C motif chemokine receptor 1 (CCR1) plays key roles in guiding leukocyte movement during immune surveillance and inflammatory responses. Targeting CCR1 is a promising approach for treating autoimmune diseases and cancers. We previously developed monoclonal antibodies against mouse CCR1 (mCCR1) (clones C1Mab-2 and C1Mab-6) for use in flow cytometry and Western blotting. However, the specific binding sites have not yet been identified. This study examined the binding epitope of C1Mab-2 and C1Mab-6 using flow cytometry. Analysis of mCCR1 mutants with altered extracellular domains showed that C1Mab-2 and C1Mab-6 bind to the N-terminal region of mCCR1. Additionally, PA-tag substitution experiments identified the epitope as 1st Met and amino acids 2-13 of mCCR1. Further alanine (or glycine) scanning within the N-terminal region (amino acids 2-13) demonstrated that Glu2, Asp5, and Phe6 are essential for recognition by C1Mab-2, and Glu2, Ile3, and Asp5 are crucial for recognition by C1Mab-6 in flow cytometry and Western blotting. These findings contribute to the understanding of mCCR1 and C1Mab interaction.
Competing Interests:	Author Disclosure StatementThe authors have no conflicts of interest.
Contributed Indexing:	Keywords: alanine scanning; epitope mapping; flow cytometry; monoclonal antibody; mouse CCR1
Substance Nomenclature:	0 (Antibodies, Monoclonal) 0 (Receptors, CCR1) 0 (Epitopes) 0 (Ccr1 protein, mouse)
Entry Date(s):	Date Created: 20260610 Date Completed: 20260702 Latest Revision: 20260702
Update Code:	20260703
DOI:	10.1177/21679436261459606
PMID:	42267894
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	2167-9436
DOI:	10.1177/21679436261459606