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A segmented electron donor dosing strategy for enhancing thiosulfate-driven partial denitrifying efficiency: Insights into sulfur oxidation pathway, electron transfer and metagenomic microbial ecology.

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Τίτλος: A segmented electron donor dosing strategy for enhancing thiosulfate-driven partial denitrifying efficiency: Insights into sulfur oxidation pathway, electron transfer and metagenomic microbial ecology.
Συγγραφείς: Wang Y; College of Civil Engineering, Fuzhou University, Fuzhou 350116, China., Huang Y; College of Civil Engineering, Fuzhou University, Fuzhou 350116, China., Yin D; Guangzhou Municipal Engineering Design and Research Institute Co., Ltd., Guangzhou 510000, China., Gong B; Changjiang Survey Planning Design and Research Co., Ltd, Wuhan, Hubei 430010, China., Fan G; College of Civil Engineering, Fuzhou University, Fuzhou 350116, China. Electronic address: fgdfz@fzu.edu.cn.
Πηγή: Bioresource technology [Bioresour Technol] 2026 Oct; Vol. 458, pp. 135106. Date of Electronic Publication: 2026 Jun 07.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE
Imprint Name(s): Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
Ιατρικοί όροι (MeSH): Denitrification*/drug effects , Sulfur*/metabolism , Thiosulfates*/pharmacology , Thiosulfates*/metabolism , Metagenomics*/methods, Electron Transport/drug effects ; Thiobacillus/metabolism ; Thiobacillus/genetics ; Oxidation-Reduction ; Electrons
Περίληψη: Thiosulfate-driven partial denitrification (TPD) is a highly efficient denitrification process that exhibits good stability when coupled with Anammox. This study aimed to enhance the performance of the TPD system by employing different electron donor dosing strategies. The data show that the NO3--N removal efficiency (NRE) and NO2--N accumulation efficiency (NAE) in the segmented dosing group reached 98 % and 90 %, respectively. The study indicates that segmented electron donor dosing significantly enhances the activity of the electron transport chain. Specifically, Complex I and Complex III are associated with electron utilization by nitrate reductase (Nar) and nitrite reductase (Nir). The increased activity of Complex I and the inhibited activity of Complex III in the segmented dosing group contribute to improved NRE and NAE. Metagenomic analysis revealed that Thiobacillus predominated and served as the key functional species for Nar, Nir, and sulfur oxidation. Combined with qPCR analysis, segmented dosing significantly increased the expression levels of functional genes and elevated the NarG/(NirK + NirS) ratio, which further facilitated the accumulation of NO2--N. Furthermore, the segmented dosing group possessed a complete sulfur oxidation pathway capable of fully oxidizing S2O32- to SO42-, suggesting a reduced metabolic potential for S0 production within the system. Overall, this study offers a potential strategy for ensuring a stable supply of nitrite in future anaerobic ammonium oxidation processes.
(Copyright © 2026 Elsevier Ltd. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Electron donor dosing strategy; Electron transport system activity; Metagenomic microbial ecology; Nitrite accumulation; Thiosulfate-driven partial denitrification system
Substance Nomenclature: 70FD1KFU70 (Sulfur)
0 (Thiosulfates)
Entry Date(s): Date Created: 20260608 Date Completed: 20260721 Latest Revision: 20260721
Update Code: 20260721
DOI: 10.1016/j.biortech.2026.135106
PMID: 42259455
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1873-2976
DOI:10.1016/j.biortech.2026.135106