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Hedyotis diffusa Willd-Scutellaria barbata D.Don Suppresses Colorectal Cancer Liver Metastasis by Targeting hsa_circ_0039933-Mediated Epithelial-Mesenchymal Transition.

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Τίτλος: Hedyotis diffusa Willd-Scutellaria barbata D.Don Suppresses Colorectal Cancer Liver Metastasis by Targeting hsa_circ_0039933-Mediated Epithelial-Mesenchymal Transition.
Συγγραφείς: Yuan S; Department of Traditional Chinese Medicine, Ganzhou People's Hospital, No.16, Meiguan Avenue, Zhanggong District, Ganzhou City, 341000, Jiangxi Province, China., Li S; Department of Traditional Chinese Medicine, Ganzhou People's Hospital, No.16, Meiguan Avenue, Zhanggong District, Ganzhou City, 341000, Jiangxi Province, China., Chen C; Department of Traditional Chinese Medicine, Ganzhou People's Hospital, No.16, Meiguan Avenue, Zhanggong District, Ganzhou City, 341000, Jiangxi Province, China. c15793478243@126.com.
Πηγή: Biochemical genetics [Biochem Genet] 2026 Oct; Vol. 64 (5), pp. 7608-7627. Date of Electronic Publication: 2026 Jun 08.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Kluwer Academic/Plenum Publishers Country of Publication: United States NLM ID: 0126611 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4927 (Electronic) Linking ISSN: 00062928 NLM ISO Abbreviation: Biochem Genet Subsets: MEDLINE
Imprint Name(s): Publication: 1999- : New York : Kluwer Academic/Plenum Publishers
Original Publication: New York, Plenum Press.
Ιατρικοί όροι (MeSH): Colorectal Neoplasms*/pathology , Colorectal Neoplasms*/drug therapy , Colorectal Neoplasms*/genetics , Colorectal Neoplasms*/metabolism , Epithelial-Mesenchymal Transition*/drug effects , Liver Neoplasms*/secondary , Liver Neoplasms*/drug therapy , Liver Neoplasms*/genetics , Liver Neoplasms*/metabolism , Hedyotis*/chemistry , RNA, Circular*/genetics , RNA, Circular*/metabolism , Drugs, Chinese Herbal*/pharmacology, Cell Proliferation/drug effects ; Cell Movement/drug effects ; Animals ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Cell Line, Tumor ; Male
Περίληψη: Colorectal cancer (CRC) liver metastasis remains a major cause of mortality, necessitating novel therapeutic strategies. Hedyotis diffusa Willd -Scutellaria barbata D.Don (HD-SB), a traditional Chinese herbal formulation, has demonstrated anti-tumor potential. However, its mechanism in CRC metastasis, particularly through the regulation of circular RNAs, remains unexplored. A CRC liver metastasis model was established in Balb/c nude mice via intrasplenic injection of HCT-15 cells. Mice received HD-SB treatment. Hematoxylin-eosin staining, Masson staining, and immunohistochemistry (Ki67 and Cytokeratin 19 [CK19]) were carried out to observe pathologic changes in CRC liver metastases. In vitro, HCT-15 cells were treated with tumor necrosis factor-α (TNF-α) to induce metastasis. hsa_circ_0039933 was knocked down and overexpressed in vitro. Transwell, wound healing, EdU, and cell counting kit-8 assays were employed to assess cell invasion, migration and proliferation, respectively. The levels of epithelial-mesenchymal transition (EMT) markers were evaluated by quantitative real-time polymerase chain reaction and western blot. HD-SB treatment dose-dependently reduced hepatic tumor nodules, fibrosis, Ki67 and CK19 expression in vivo, with high-dose HD-SB showing superior efficacy. EMT reverse (upregulated E-cadherin, downregulated N-cadherin/Vimentin) and hsa_circ_0039933 suppression were observed in HD-SB treated CRC mouse. In vitro, HD-SB attenuated TNF-α-induced proliferation, migration, and invasion, synergizing with hsa_circ_0039933 knockdown to suppress EMT and metastatic phenotypes. Conversely, hsa_circ_0039933 overexpression exacerbated TNF-α-driven aggressiveness, which was effectively counteracted by HD-SB. Collectively, HD-SB inhibits CRC liver metastasis by targeting hsa_circ_0039933 and modulating EMT. These findings highlight HD-SB as a promising circRNA-directed therapeutic agent for metastatic CRC.
(© 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Competing Interests: Declarations. Competing Interests: The authors declare no competing interests. Ethical Approval and Consent to Participate: The experiments conformed to the Guide for the Care and Use of Laboratory Animals. Animal study has been approved by the Animal Ethics Committee of Nanchang University (SYXK (Ganzhou) 2021-0004). All methods are reported in accordance with ARRIVE guidelines.
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Grant Information: 202510095 Jiangxi Provincial Health Technology Project; 2023LNS17457 Ganzhou Municipal Science and Technology Project; BSQD2020011 Doctoral research start-up fund project of Ganzhou People's Hospital
Contributed Indexing: Keywords: Hedyotis diffusa Willd-Scutellaria barbata D.Don; Colorectal cancer; Epithelial-mesenchymal transition; Hsa_circ_0039933; Liver metastasis
Substance Nomenclature: 0 (RNA, Circular)
0 (Drugs, Chinese Herbal)
Entry Date(s): Date Created: 20260608 Date Completed: 20260906 Latest Revision: 20260906
Update Code: 20260907
DOI: 10.1007/s10528-026-11392-4
PMID: 42258086
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1573-4927
DOI:10.1007/s10528-026-11392-4