Academic Journal
Utility of ultrasensitive, US Food and Drug Administration-approved κ and λ dual in situ hybridization assay in the diagnosis of Hodgkin lymphomas.
| Τίτλος: | Utility of ultrasensitive, US Food and Drug Administration-approved κ and λ dual in situ hybridization assay in the diagnosis of Hodgkin lymphomas. |
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| Συγγραφείς: | Eltahir M; Department of Pathology, Stanford University School of Medicine, Stanford, CA, United States., Young K; Department of Pathology, Stanford University School of Medicine, Stanford, CA, United States., Younes S; Department of Pathology, Stanford University School of Medicine, Stanford, CA, United States., Zhao S; Department of Pathology, Stanford University School of Medicine, Stanford, CA, United States., Silva O; Department of Pathology, Stanford University School of Medicine, Stanford, CA, United States., Natkunam Y; Department of Pathology, Stanford University School of Medicine, Stanford, CA, United States. |
| Πηγή: | American journal of clinical pathology [Am J Clin Pathol] 2026 Jun 04; Vol. 165 (6). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0370470 Publication Model: Print Cited Medium: Internet ISSN: 1943-7722 (Electronic) Linking ISSN: 00029173 NLM ISO Abbreviation: Am J Clin Pathol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2016- : Oxford : Oxford University Press Original Publication: Philadelphia [etc.] Lippincott [etc.] |
| Ιατρικοί όροι (MeSH): | Hodgkin Disease*/diagnosis , In Situ Hybridization*/methods , Immunoglobulin kappa-Chains*/genetics , Immunoglobulin kappa-Chains*/analysis , Immunoglobulin lambda-Chains*/genetics , Immunoglobulin lambda-Chains*/analysis, B-Lymphocytes/pathology ; Biomarkers, Tumor/analysis ; Reed-Sternberg Cells/pathology ; Humans ; United States ; Female ; United States Food and Drug Administration ; Male ; Middle Aged ; Adult ; Aged ; Adolescent ; Aged, 80 and over ; Young Adult |
| Περίληψη: | Objectives: Determination of B-cell clonality in formalin-fixed, paraffin-embedded tissues is challenging, especially in cases where tumor B cells are sparse. Flow cytometry is of limited utility in Hodgkin lymphomas, at least in part due to the rarity and fragility of the malignant cells. Methods: We used a recently US Food and Drug Administration-approved, ultrasensitive κ and λ dual in situ hybridization assay (K/L dual ISH; Roche/Ventana Medical Systems) to evaluate B-cell clonality in 184 classic Hodgkin lymphomas (CHLs) and 113 nodular lymphocyte-predominant Hodgkin lymphomas (NLPHLs). Results: Five CHLs and 11 NLPHLs were not scorable due to preanalytical variables. Of the 179 scorable CHL cases, none showed light chain messenger RNA expression in the Hodgkin/Reed-Sternberg cells, whereas 89% of NLPHLs (91/102) exhibited light chain expression in lymphocyte-predominant (LP) cells. Among NLPHLs, 66% (68/102) showed κ restriction, 20% (21/102) showed λ restriction, and 1.9% (2/102) showed dual κ and λ expression in the LP cells. There was no appreciable light chain expression in 10% (11/102) of cases. Conclusions: Although morphology and immunohistochemistry are usually sufficient to separate CHL from NLPHL, determining light chain restriction by K/L dual ISH could provide a valuable adjunct in challenging cases, potentially improving diagnostic accuracy and patient outcomes. (© The Author(s) 2026. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.) |
| Contributed Indexing: | Keywords: HRS cells; Hodgkin lymphoma; LP cells; in situ hybridization; nodular lymphocyte predominant; κ; λ |
| Substance Nomenclature: | 0 (Immunoglobulin kappa-Chains) 0 (Immunoglobulin lambda-Chains) 0 (Biomarkers, Tumor) |
| Entry Date(s): | Date Created: 20260608 Date Completed: 20260612 Latest Revision: 20260730 |
| Update Code: | 20260730 |
| DOI: | 10.1093/ajcp/aqag043 |
| PMID: | 42252788 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1943-7722 |
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| DOI: | 10.1093/ajcp/aqag043 |