Academic Journal
Metabolic division of labor drives synergistic production of biomass, lipid and α-tocopherol in Euglena gracilis-Chlorella pyrenoidosa co-cultures.
| Τίτλος: | Metabolic division of labor drives synergistic production of biomass, lipid and α-tocopherol in Euglena gracilis-Chlorella pyrenoidosa co-cultures. |
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| Συγγραφείς: | Wang Z; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei 230009, China., Tian Q; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei 230009, China., Fu L; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei 230009, China., Xing X; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei 230009, China., Liu W; Intelligent Control and Compute Vision Lab, Hefei University, Hefei 230601, China., Liu C; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei 230009, China. Electronic address: changhong22@hfut.edu.cn., Zheng L; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei 230009, China. Electronic address: lzheng@hfut.edu.cn. |
| Πηγή: | Bioresource technology [Bioresour Technol] 2026 Oct; Vol. 458, pp. 135066. Date of Electronic Publication: 2026 Jun 04. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991- |
| Ιατρικοί όροι (MeSH): | Euglena gracilis*/metabolism , Euglena gracilis*/growth & development , alpha-Tocopherol*/metabolism , Chlorella*/metabolism , Chlorella*/growth & development , Lipids*/biosynthesis , Biomass*, Coculture Techniques/methods ; Lipid Metabolism |
| Περίληψη: | Microalgal co-culture is a promising strategy to enhance biomass and high-value metabolite production, yet the underlying metabolic coordination remains to be fully elucidated. This study developed a synergistic co-culture system using Euglena gracilis (EG) and Chlorella pyrenoidosa (CP) to maximize biomass, lipid, and α-tocopherol yields. Under an optimized inoculation ratio of EG:CP = 3:7 in TAP medium, the co-culture achieved a biomass concentration of 1.23 g/L, lipid productivity of 163.7 mg/L/d, and α-tocopherol yield of 1976.28 μg/L, corresponding to 1.18-1.52-fold, 1.58-2.81-fold, and 2.13-9.90-fold increases relative to monoculture systems. The system produced a lipid profile enriched in polyunsaturated fatty acids (76.81%), specifically linolenic acid (C18:3, 56.47%). Notably, the logarithmic growth phase was reduced by 50% (3 days), significantly enhancing cultivation efficiency. Multi-omics analysis revealed a clear metabolic division of labor, wherein EG primarily enhanced light-harvesting capacity and antioxidative metabolite secretion, while CP intensified electron transport activity, enhanced carbon fixation capacity, and released lipid-associated metabolites, collectively driving process intensification in terms of biomass and lipid productivity. Moreover, exogenous α-lipoic acid supplementation further amplified biomass accumulation (1.34 g/L), lipid productivity (182.4 mg/L/d), and α-tocopherol production (2320.48 μg/L). These findings demonstrate that metabolic cooperation in microalgal consortia can significantly improve bioresource recovery, offering a scalable strategy for high-efficiency microalgal biorefineries. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Co-production; Linolenic acid; Microalgae; Mutualism; Secondary Metabolites |
| Substance Nomenclature: | H4N855PNZ1 (alpha-Tocopherol) 0 (Lipids) |
| Entry Date(s): | Date Created: 20260605 Date Completed: 20260721 Latest Revision: 20260721 |
| Update Code: | 20260721 |
| DOI: | 10.1016/j.biortech.2026.135066 |
| PMID: | 42248260 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-2976 |
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| DOI: | 10.1016/j.biortech.2026.135066 |