Academic Journal
Efficient induction of EPSCs via episomal reprogramming: a functionally equivalent yet transcriptionally specialized alternative to EPSCs.
| Τίτλος: | Efficient induction of EPSCs via episomal reprogramming: a functionally equivalent yet transcriptionally specialized alternative to EPSCs. |
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| Συγγραφείς: | Zheng R; Shenzhen Key Laboratory of Metabolic Health, Shenzhen Metabolism and Reproductive Targeted Delivery Proof-of-Concept Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, No. 1068 Xueyuan Avenue, Shenzhen University Town, Xili, Nanshan District, Shenzhen, China; Shenzhen Beike Biotechnology Co., Ltd., Shenzhen 518054, China., Zhang M; Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Science, Hangzhou 310024, China; Shenzhen Beike Biotechnology Co., Ltd., Shenzhen 518054, China., Cheng Y; Shenzhen Beike Biotechnology Co., Ltd., Shenzhen 518054, China., Wen J; Shenzhen Beike Biotechnology Co., Ltd., Shenzhen 518054, China., Zhao X; Shenzhen Key Laboratory of Metabolic Health, Shenzhen Metabolism and Reproductive Targeted Delivery Proof-of-Concept Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, No. 1068 Xueyuan Avenue, Shenzhen University Town, Xili, Nanshan District, Shenzhen, China., Xiao D; Shenzhen Beike Biotechnology Co., Ltd., Shenzhen 518054, China., Xu J; Shenzhen Beike Biotechnology Co., Ltd., Shenzhen 518054, China., Pei K; Shenzhen Beike Biotechnology Co., Ltd., Shenzhen 518054, China., Wang L; Shenzhen Beike Biotechnology Co., Ltd., Shenzhen 518054, China., Xu J; Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Science, Hangzhou 310024, China., Zhang J; Shenzhen Key Laboratory of Metabolic Health, Shenzhen Metabolism and Reproductive Targeted Delivery Proof-of-Concept Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, No. 1068 Xueyuan Avenue, Shenzhen University Town, Xili, Nanshan District, Shenzhen, China; Faculty of Pharmaceutical Sciences, Shenzhen University of Advanced Technology, 1068 Xueyuan Avenue, Shenzhen University Town, Shenzhen, China; Sino-European Center of Biomedicine and Health, No. 7, Qingshuihe 3rd Road, Luohu District, Shenzhen, China. Electronic address: jian.zhang@siat.ac.cn., Cai C; Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Science, Hangzhou 310024, China; Shenzhen Beike Biotechnology Co., Ltd., Shenzhen 518054, China. Electronic address: cheguo_cai@ucas.ac.cn., Gao Y; Shenzhen Beike Biotechnology Co., Ltd., Shenzhen 518054, China; Shenzhen Beikeyuan Cell Technology Co., Ltd., Shenzhen 518054, China. Electronic address: yunfeng@beike.cc. |
| Πηγή: | Stem cell research [Stem Cell Res] 2026 Aug; Vol. 94, pp. 104027. Date of Electronic Publication: 2026 Jun 03. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: England NLM ID: 101316957 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7753 (Electronic) Linking ISSN: 18735061 NLM ISO Abbreviation: Stem Cell Res Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Kidlington, Oxford : Elsevier |
| Ιατρικοί όροι (MeSH): | Cellular Reprogramming*/genetics , Plasmids*/metabolism , Plasmids*/genetics , Pluripotent Stem Cells*/metabolism , Pluripotent Stem Cells*/cytology, Induced Pluripotent Stem Cells/metabolism ; Induced Pluripotent Stem Cells/cytology ; Leukocytes, Mononuclear/cytology ; Leukocytes, Mononuclear/metabolism ; Humans ; Cell Differentiation |
| Περίληψη: | This study establishes a streamlined one-step strategy using optimized episomal plasmid reprogramming to directly generate extended pluripotent stem cells (dEPSCs) from human peripheral blood mononuclear cells (PBMCs), bypassing the induced pluripotent stem cell(iPSCs) intermediary phase and reducing 50% of the generation compared to conventional protocols. Functional analyses confirm that dEPSCs (direct extended pluripotent stem cells), exhibit genomic stability, bidirectional developmental potential, and shared similar molecular characteristics with traditional extended pluripotent stem cells (EPSCs), Collectively, this strategy provides a robust and scalable platform for pluripotent stem cell research, with potential applications in disease modeling, lineage-specific differentiation, and regenerative medicine. (Copyright © 2026 The Authors. Published by Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Differentiation; Episomal plasmid reprogramming; Extended pluripotent stem cells; Pluripotent analysis; Transcriptome analysis |
| Entry Date(s): | Date Created: 20260605 Date Completed: 20260614 Latest Revision: 20260614 |
| Update Code: | 20260615 |
| DOI: | 10.1016/j.scr.2026.104027 |
| PMID: | 42248118 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1876-7753 |
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| DOI: | 10.1016/j.scr.2026.104027 |