Academic Journal
Bacteria and viruses associated with antibiotic resistome in hyperthermophilic co-composting of cow manure and mushroom residue.
| Τίτλος: | Bacteria and viruses associated with antibiotic resistome in hyperthermophilic co-composting of cow manure and mushroom residue. |
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| Συγγραφείς: | Zhou X; Guangdong Key Laboratory of Environmental Pollution and Health, College of Environment and Climate, Jinan University, Guangzhou 511443, China., Yu Z; School of Environment and Civil Engineering, Dongguan University of Technology, Dongguan 523808, China. Electronic address: yuzhen@dgut.edu.cn., Liao H; Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China., Wang Y; Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China., Zhuang L; Guangdong Key Laboratory of Environmental Pollution and Health, College of Environment and Climate, Jinan University, Guangzhou 511443, China. Electronic address: zhuangli@jnu.edu.cn., Zhou S; Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China. |
| Πηγή: | Bioresource technology [Bioresour Technol] 2026 Oct; Vol. 458, pp. 135075. Date of Electronic Publication: 2026 Jun 02. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991- |
| Ιατρικοί όροι (MeSH): | Manure*/microbiology , Manure*/virology , Composting*/methods , Bacteria*/genetics , Bacteria*/drug effects , Drug Resistance, Microbial*/genetics , Viruses*/genetics , Drug Resistance, Bacterial*/genetics , Agaricales*, Animals ; Cattle ; Genes, Bacterial |
| Περίληψη: | Antibiotic resistance in livestock-derived wastes represents a critical environmental and public health concern. Here, we applied genome-resolved metagenomic analysis to characterize antibiotic resistance genes (ARGs), antibiotic-resistant bacteria (ARB), and associated viral communities during co-composting of cow manure and mushroom residue. By day 20, hyperthermophilic composting (HTC) achieved markedly higher ARG removal (93%) than conventional thermophilic composting (TC, 84%). This enhanced performance was associated with the enrichment of thermophilic taxa (e.g., Bacillaceae and Sporolactobacillaceae) and the suppression of mesophilic ARG reservoirs (e.g., Enterobacteriaceae and Pseudomonadaceae). Genome-resolved analysis further revealed that a majority of multidrug-resistant ARB were eliminated during HTC, particularly Klebsiella pneumoniae and Escherichia coli harboring diverse ARGs and virulence factor genes. These high-risk pathogens were predicted to be targeted by a subset of lytic phages, including those affiliated with Autographiviridae and Schitoviridae, suggesting a potential role of lytic phages in suppressing resistance- and virulence-associated ARB. Collectively, these findings provide genome-resolved insights into the coordinated roles of thermophile-driven suppression and phage-mediated predation of ARB in ARG removal, highlighting HTC as a promising strategy for safer manure recycling and resistance risk mitigation. (Copyright © 2026. Published by Elsevier Ltd.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Antibiotic resistance genes; Antibiotic-resistant bacteria; Bacteriophages; Hyperthermophilic composting; Virulence factor genes |
| Substance Nomenclature: | 0 (Manure) |
| Entry Date(s): | Date Created: 20260603 Date Completed: 20260721 Latest Revision: 20260721 |
| Update Code: | 20260721 |
| DOI: | 10.1016/j.biortech.2026.135075 |
| PMID: | 42235698 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-2976 |
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| DOI: | 10.1016/j.biortech.2026.135075 |