Academic Journal
NADES-assisted sustainable extraction of total protein content from Achyranthes bidentata seeds and their biochemical characterization.
| Τίτλος: | NADES-assisted sustainable extraction of total protein content from Achyranthes bidentata seeds and their biochemical characterization. |
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| Συγγραφείς: | Salunkhe PK; Department of Technology, Shivaji University, Kolhapur, 416004, Maharashtra, India. Electronic address: premrajsalunkhe09@gmail.com. |
| Πηγή: | Journal of pharmacological and toxicological methods [J Pharmacol Toxicol Methods] 2026 Aug; Vol. 140, pp. 108429. Date of Electronic Publication: 2026 Jun 01. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: United States NLM ID: 9206091 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-488X (Electronic) Linking ISSN: 10568719 NLM ISO Abbreviation: J Pharmacol Toxicol Methods Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: New York, NY : Elsevier, c1992- |
| Ιατρικοί όροι (MeSH): | Seeds*/chemistry , Achyranthes*/chemistry , Plant Proteins*/isolation & purification , Plant Proteins*/chemistry , Deep Eutectic Solvents*/chemistry , Plant Extracts*/chemistry, Flavonoids/chemistry ; Flavonoids/isolation & purification ; Phenols/chemistry ; Green Chemistry Technology/methods ; Lactic Acid/chemistry ; Solvents/chemistry ; Glycerol/chemistry |
| Περίληψη: | This study evaluates Natural Deep Eutectic Solvents (NADES) as sustainable alternatives for the extraction of proteins and phytochemicals from Achyranthes bidentata seeds. Six lactic acid-based NADES formulations were compared against water and ethanol using ultrasound-assisted extraction. Total protein, total phenolic content (TPC), and total flavonoid content (TFC) were quantified. NADES significantly outperformed conventional solvents in extraction efficiency. The binary NADES N1 (lactic acid: glycerol, 1:1) achieved the highest protein concentration (318.55 ± 24.57 μg/mL). In contrast, the ternary NADES N5 (lactic acid: glycerol: choline chloride, 2:1:1) exhibited the highest efficiency for secondary metabolites, yielding a TPC of 16.33 ± 4.61 mg GAE/g and TFC of 409.62 ± 60.47 mg QE/g. These findings demonstrate the tunable nature of NADES for targeted biomolecule recovery, validating A. bidentata seeds as a promising source of functional ingredients utilizing green extraction methodologies. (Copyright © 2026 Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of competing interest We have no financial or personal interest in any organization or entity that could be perceived as influencing the content of this manuscript. There are no conflicts of interest to disclose regarding the research or the findings presented in this paper. |
| Contributed Indexing: | Keywords: Achyranthes bidentata; Green chemistry; Natural deep eutectic solvents (NADES); Protein extraction; Ultrasound-assisted extraction (UAE) |
| Substance Nomenclature: | 0 (Plant Proteins) 0 (Deep Eutectic Solvents) 0 (Plant Extracts) 0 (Flavonoids) 0 (Phenols) 33X04XA5AT (Lactic Acid) 0 (Solvents) PDC6A3C0OX (Glycerol) |
| Entry Date(s): | Date Created: 20260601 Date Completed: 20260806 Latest Revision: 20260806 |
| Update Code: | 20260807 |
| DOI: | 10.1016/j.vascn.2026.108429 |
| PMID: | 42225216 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-488X |
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| DOI: | 10.1016/j.vascn.2026.108429 |