Academic Journal
Προβολή σε EDS

PGR5 is needed for regulation of ATP synthase in plant chloroplasts.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: PGR5 is needed for regulation of ATP synthase in plant chloroplasts.
Συγγραφείς: Nikkanen L; Molecular Plant Biology, Department of Life Technologies, University of Turku, Finland. Electronic address: lenikk@utu.fi., Woodford R; School of Biological Sciences, Monash University, Australia., Andersin E; Molecular Plant Biology, Department of Life Technologies, University of Turku, Finland., Wey LT; Molecular Plant Biology, Department of Life Technologies, University of Turku, Finland., Ermakova M; School of Biological Sciences, Monash University, Australia., Rintamäki E; Molecular Plant Biology, Department of Life Technologies, University of Turku, Finland.
Πηγή: Biochimica et biophysica acta. Bioenergetics [Biochim Biophys Acta Bioenerg] 2026 Nov 01; Vol. 1867 (4), pp. 149596. Date of Electronic Publication: 2026 May 29.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101731706 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-2650 (Electronic) Linking ISSN: 00052728 NLM ISO Abbreviation: Biochim Biophys Acta Bioenerg Subsets: MEDLINE
Imprint Name(s): Original Publication: Amsterdam : Elsevier
Ιατρικοί όροι (MeSH): Arabidopsis*/genetics , Arabidopsis*/enzymology , Arabidopsis*/metabolism , Arabidopsis Proteins*/metabolism , Arabidopsis Proteins*/genetics , Chloroplasts*/enzymology , Chloroplasts*/metabolism , Chloroplasts*/genetics , Photosynthetic Reaction Center Complex Proteins*/metabolism , Photosynthetic Reaction Center Complex Proteins*/genetics , Chloroplast Proton-Translocating ATPases*/metabolism, Light ; Oxidation-Reduction ; Proton-Motive Force ; Electron Transport ; Photosynthesis
Περίληψη: Regulation of proton motive force (pmf) via ATP synthase activity is a critical mechanism by which photosynthetic organisms maintain redox homeostasis and control the activation and inactivation of photoprotective responses under fluctuating light conditions. Here, we used time-resolved electrochromic shift measurements to investigate pmf dynamics in the C3 model plant Arabidopsis thaliana and the C4 model grass Setaria viridis. Our results reveal that ATP synthase is dynamically regulated during light fluctuations, but in Arabidopsis this regulation could not be explained by the established light-induced reduction of the CF₁γ subunit by thioredoxins, suggesting alternative control mechanisms. The PROTON GRADIENT REGULATION 5 (PGR5) protein, previously proposed to facilitate cyclic electron transport (CET) in plants and algae, also has a potential role in regulation of ATP synthase. We therefore investigated pmf dynamics, cytochrome f redox changes, and linear and cyclic PSI electron transport rates in WT and pgr5 knock-out mutants and revealed that while PGR5 was not required for CET, it was needed for downregulating ATP synthase under high irradiance in both species. Furthermore, in Arabidopsis disturbance of thiol redox regulation by addition of N-ethylmaleimide resulted in downregulation of ATP synthase conductivity in WT but not in pgr5 mutants, and PGR5 interacted with CF₁γ in planta. We suggest that PGR5 functions as a conserved thiol redox state dependent inhibitor of chloroplast ATP synthase under high light, contributing to pmf retention and photoprotection.
(Copyright © 2026. Published by Elsevier B.V.)
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Lauri Nikkanen reports financial support was provided by Research Council of Finland. Eevi Rintamäki reports financial support was provided by Research Council of Finland. Eevi Rintamäki reports financial support was provided by Jane and Aatos Erkko Fundation. Laura T Wey reports financial support was provided by Novo Nordisk Foundation. Maria Ermakova reports financial support was provided by Australian Research Council. Maria Ermakova reports financial support was provided by Australian Academy of Science. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: ATP synthase; Arabidopsis; Chloroplast; Electrochromic shift; Photosynthesis; Proton motive force; Thioredoxin
Substance Nomenclature: 0 (Arabidopsis Proteins)
0 (PGR5 protein, Arabidopsis)
0 (Photosynthetic Reaction Center Complex Proteins)
EC 3.6.3.- (Chloroplast Proton-Translocating ATPases)
Entry Date(s): Date Created: 20260530 Date Completed: 20260909 Latest Revision: 20260909
Update Code: 20260910
DOI: 10.1016/j.bbabio.2026.149596
PMID: 42217716
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1879-2650
DOI:10.1016/j.bbabio.2026.149596