Academic Journal
Carpesium abrotanoides extract attenuates chronic obstructive pulmonary disease-associated airway inflammation through inhibition of MAPK signaling.
| Τίτλος: | Carpesium abrotanoides extract attenuates chronic obstructive pulmonary disease-associated airway inflammation through inhibition of MAPK signaling. |
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| Συγγραφείς: | Han YK; KM Science Research Division, Korea Institute of Oriental Medicine, Daejeon, 34054, Republic of Korea., Kim SY; KM Science Research Division, Korea Institute of Oriental Medicine, Daejeon, 34054, Republic of Korea., Yang WK; DJU Industry-University Cooperation Foundation, Daejeon University, Daejeon, 34520, Republic of Korea., Ji YE; DJU Industry-University Cooperation Foundation, Daejeon University, Daejeon, 34520, Republic of Korea., Kim SH; DJU Industry-University Cooperation Foundation, Daejeon University, Daejeon, 34520, Republic of Korea., Kim DS; KM Science Research Division, Korea Institute of Oriental Medicine, Daejeon, 34054, Republic of Korea., Sung YY; KM Science Research Division, Korea Institute of Oriental Medicine, Daejeon, 34054, Republic of Korea. Electronic address: yysung@kiom.re.kr. |
| Πηγή: | Journal of ethnopharmacology [J Ethnopharmacol] 2026 Oct 28; Vol. 369, pp. 121894. Date of Electronic Publication: 2026 May 26. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Limerick : Elsevier Sequoia Original Publication: Lausanne, Elsevier Sequoia. |
| Ιατρικοί όροι (MeSH): | Plant Extracts*/pharmacology , Plant Extracts*/therapeutic use , Pulmonary Disease, Chronic Obstructive*/drug therapy , Pulmonary Disease, Chronic Obstructive*/pathology , Asteraceae*/chemistry , Anti-Inflammatory Agents*/pharmacology , Anti-Inflammatory Agents*/therapeutic use , Anti-Inflammatory Agents*/isolation & purification , MAP Kinase Signaling System*/drug effects , Pneumonia*/drug therapy, Bronchoalveolar Lavage Fluid/cytology ; Lung/drug effects ; Lung/pathology ; Cytokines/metabolism ; Mitogen-Activated Protein Kinases/metabolism ; Smoke/adverse effects ; Nitric Oxide/metabolism ; Animals ; Mice, Inbred BALB C ; Mice ; Lipopolysaccharides ; RAW 264.7 Cells ; Male ; Disease Models, Animal ; Female |
| Περίληψη: | Ethnopharmacological Relevance: Carpesium abrotanoides L. (Asteraceae) is traditionally used to treat inflammatory conditions including bronchitis, sore throat, and tonsillitis. However, the evidence supporting its potential in chronic obstructive pulmonary disease (COPD) treatment remains limited. Aim of the Study: To evaluate the effects of C. abrotanoides extract (CA) in a cigarette smoke extract/lipopolysaccharide (CSE/LPS)-induced mouse model relevant to COPD and to investigate CA's association with modulation of inflammatory mediators and MAPK signaling. Materials and Methods: CA was chemically profiled using LC-QTOF MS/MS. Anti-inflammatory activity was assessed in LPS-stimulated RAW 264.7 macrophages by measuring viability, nitric oxide (NO), cytokine release, and MAPK phosphorylation. BALB/c mice exposed to CSE/LPS and received oral CA (25 or 100 mg/kg) or dexamethasone (3 mg/kg). Airway inflammation and lung injury were evaluated via bronchoalveolar lavage fluid (BALF) cell counts and mediator levels, flow cytometry, lung histopathology with semi-quantitative scoring, immunofluorescence (TNF-α, CXCL1, IRAK1), lung MAPK phosphorylation, and qRT-PCR. Antitussive and expectorant properties were evaluated using ammonia-induced cough and phenol red secretion assays, respectively. Results: Ten major constituents of CA were identified. In macrophages, CA reduced LPS-induced NO, pro-inflammatory mediators, and MAPK phosphorylation. In CSE/LPS-exposed mice, CA decreased BALF cellularity, neutrophil infiltration, and inflammatory mediator levels; improved lung histopathology; reduced TNF-α/CXCL1/IRAK1 signal; and inhibited inflammatory and mucus/remodeling-related genes, including TRPA1. CA also reduced cough frequency and increased phenol red secretion. Conclusion: CA attenuated airway inflammation and lung injury in a preclinical model relevant to COPD, with effects consistent with modulation of IRAK1-related signaling and reduced MAPK activation. Further studies in chronic models are warranted. (Copyright © 2026 The Authors. Published by Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Airway inflammation; Carpesium abrotanoides; Chronic obstructive pulmonary disease; Cigarette smoke extract–lipopolysaccharide; MAPK signaling |
| Substance Nomenclature: | 0 (Plant Extracts) 0 (Anti-Inflammatory Agents) 0 (Lipopolysaccharides) 0 (Cytokines) EC 2.7.11.24 (Mitogen-Activated Protein Kinases) 0 (Smoke) 31C4KY9ESH (Nitric Oxide) |
| Entry Date(s): | Date Created: 20260527 Date Completed: 20260612 Latest Revision: 20260612 |
| Update Code: | 20260612 |
| DOI: | 10.1016/j.jep.2026.121894 |
| PMID: | 42202916 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1872-7573 |
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| DOI: | 10.1016/j.jep.2026.121894 |