Academic Journal
CsMYB6 promotes crocins biosynthesis in response to JA signaling in Crocus sativus L.
| Τίτλος: | CsMYB6 promotes crocins biosynthesis in response to JA signaling in Crocus sativus L. |
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| Συγγραφείς: | Wang S; State Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou, 311300, China., Lin C; State Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou, 311300, China., Shi X; Huzhou academy of Agricultural Science, Huzhou, 313000, China., Zhou A; State Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou, 311300, China., Chen T; State Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou, 311300, China., Zheng Y; State Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou, 311300, China., Shao Q; State Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou, 311300, China., Zhang L; College of Life Sciences and Medicine, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, Zhejiang Sci-Tech University, Hangzhou, 310018, China., Ding L; Hangzhou Linan District Agricultural and Forestry Technology Extension Center, Hangzhou, 311300, China. Electronic address: 13727278@qq.com., Xing B; State Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou, 311300, China. Electronic address: xingbingcong@163.com. |
| Πηγή: | Journal of plant physiology [J Plant Physiol] 2026 Aug; Vol. 323, pp. 154801. Date of Electronic Publication: 2026 May 21. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Urban & Fischer Country of Publication: Germany NLM ID: 9882059 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-1328 (Electronic) Linking ISSN: 01761617 NLM ISO Abbreviation: J Plant Physiol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Jena, Germany : Urban & Fischer Original Publication: Stuttgart ; New York : G. Fischer, c1984- |
| Ιατρικοί όροι (MeSH): | Carotenoids*/metabolism , Cyclopentanes*/metabolism , Crocus*/metabolism , Crocus*/genetics , Oxylipins*/metabolism , Plant Proteins*/metabolism , Plant Proteins*/genetics , Transcription Factors*/metabolism , Transcription Factors*/genetics , Plant Growth Regulators*/metabolism , Signal Transduction*, Gene Expression Regulation, Plant ; Promoter Regions, Genetic |
| Περίληψη: | Crocins has been identified as the key bioactive compound in saffron. The plant hormone jasmonate promotes the accumulation of crocins in saffron, but its regulatory mechanism remains unclear. MYB transcription factors are essential for regulating plant development and metabolic processes. In this study, a MeJA-responsive R2R3-MYB transcription factor, CsMYB6, was identified. The expression of CsMYB6 showed a strong correlation with the expression of key genes involved in crocins biosynthesis. Overexpression of CsMYB6 in Crocus sativus stigmas enhanced crocin content and upregulated the expression levels of key enzyme genes involved in crocins biosynthesis. Yeast one-hybrid assays confirmed the interaction between CsMYB6 and CsZ-ISO3/CsCCD2 promoters. Dual-LUC assays demonstrated that CsMYB6 binds to and activates the promoter of CsZ-ISO3/CsCCD2. These findings reveal a novel regulatory role of CsMYB6 in MeJA-mediated crocins biosynthesis and propose a "MeJA-CsMYB6-CsZ-ISO3/CsCCD2-crocins" regulatory model, enriching the regulatory network of crocins biosynthesis and laying a foundation for further exploration of the molecular mechanisms underlying saffron secondary metabolism. (Copyright © 2026 Elsevier GmbH. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Crocins; Crocus sativus L; CsMYB6; JA signaling; Secondary metabolism regulation |
| Substance Nomenclature: | 36-88-4 (Carotenoids) 0 (Cyclopentanes) 877GWI46C2 (crocin) 6RI5N05OWW (jasmonic acid) 0 (Oxylipins) 0 (Plant Proteins) 0 (Transcription Factors) 0 (Plant Growth Regulators) |
| Entry Date(s): | Date Created: 20260527 Date Completed: 20260626 Latest Revision: 20260626 |
| Update Code: | 20260626 |
| DOI: | 10.1016/j.jplph.2026.154801 |
| PMID: | 42202640 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1618-1328 |
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| DOI: | 10.1016/j.jplph.2026.154801 |