Academic Journal
Discovery of Q-Markers of Mudan Granules by Chemical Profile, Pharmacokinetics, Neuroprotective Effect Evaluation, and Network Pharmacology.
| Τίτλος: | Discovery of Q-Markers of Mudan Granules by Chemical Profile, Pharmacokinetics, Neuroprotective Effect Evaluation, and Network Pharmacology. |
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| Συγγραφείς: | Wang Z; College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.; Innovation Center of Translational Pharmacy, Jinhua Institute of Zhejiang University, Jinhua, China., Zhang S; College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.; Innovation Center of Translational Pharmacy, Jinhua Institute of Zhejiang University, Jinhua, China., Hu C; College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.; Innovation Center of Translational Pharmacy, Jinhua Institute of Zhejiang University, Jinhua, China., Wang S; College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.; Innovation Center of Translational Pharmacy, Jinhua Institute of Zhejiang University, Jinhua, China. |
| Πηγή: | Biomedical chromatography : BMC [Biomed Chromatogr] 2026 Jul; Vol. 40 (7), pp. e70494. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley Country of Publication: England NLM ID: 8610241 Publication Model: Print Cited Medium: Internet ISSN: 1099-0801 (Electronic) Linking ISSN: 02693879 NLM ISO Abbreviation: Biomed Chromatogr Subsets: MEDLINE |
| Imprint Name(s): | Publication: 1990- : Chichester : Wiley Original Publication: London : Heyden & Son, c1986-1990 |
| Ιατρικοί όροι (MeSH): | Drugs, Chinese Herbal*/pharmacokinetics , Drugs, Chinese Herbal*/chemistry , Drugs, Chinese Herbal*/pharmacology , Drugs, Chinese Herbal*/analysis , Neuroprotective Agents*/pharmacokinetics , Neuroprotective Agents*/chemistry , Neuroprotective Agents*/pharmacology , Neuroprotective Agents*/analysis, Chromatography, High Pressure Liquid/methods ; Liquid Chromatography-Mass Spectrometry/methods ; Biomarkers/analysis ; Schwann Cells/drug effects ; Animals ; Rats ; Male ; Rats, Sprague-Dawley ; Network Pharmacology |
| Περίληψη: | Mudan granules (MDs) are a widely used Chinese patent medicine for treating diabetic peripheral neuropathy (DPN). TCM quality is the basis to ensure its efficacy; however, the current quality standard for MD relies on the control of only two constituents derived from the minister drugs. Therefore, it is necessary to establish a more comprehensive quality evaluation system based on rational quality markers (Q-markers). In this study, Q-markers of MD were discovered through systematic evaluations. Thirty-seven compounds were newly characterized in MD by UPLC-QTOF-MS. Among all the 173 constituents in MD, 64 were found to be absorbed into the bloodstream and/or sciatic nerve of rats in prototype. The contents of 24 candidate Q-markers were determined, and the pharmacokinetics features of nine ones were analyzed by two developed UPLC-MS methods. Six candidate Q-markers were first found to exhibit protective effects on Schwann cells against the injury of high glucose/lipid. Finally, nine Q-markers were screened, and network pharmacology analysis showed that the enriched pathways for these nine Q-markers covered the major DPN-related pathways for the full set of 173 constituents. The results provide important evidence for improving the quality control system of MD and reference for discovering Q-markers of other TCM prescriptions. (© 2026 John Wiley & Sons Ltd.) |
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| Grant Information: | 82274088 National Natural Science Foundation of China; 2024-1-093 Jinhua Science and Technology Program |
| Contributed Indexing: | Keywords: Mudan granules; Q‐markers; network pharmacology; neuroprotective effect; pharmacokinetics |
| Substance Nomenclature: | 0 (Drugs, Chinese Herbal) 0 (Neuroprotective Agents) 0 (Biomarkers) |
| Entry Date(s): | Date Created: 20260526 Date Completed: 20260717 Latest Revision: 20260717 |
| Update Code: | 20260717 |
| DOI: | 10.1002/bmc.70494 |
| PMID: | 42187695 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1099-0801 |
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| DOI: | 10.1002/bmc.70494 |