Academic Journal
Chalcogenated phosphine derivatives as urease inhibitors for agricultural: soil application and biophysical studies.
| Τίτλος: | Chalcogenated phosphine derivatives as urease inhibitors for agricultural: soil application and biophysical studies. |
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| Συγγραφείς: | Enes KB; Departamento de Química, ICEx, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil., Santos EFA; Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Maceió 57072-900, AL, Brazil., Viana LPS; Departamento de Química, ICEx, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil., Júnior MMO; Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Maceió 57072-900, AL, Brazil., Batista PHJ; Departamento de Química, ICEx, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; Instituto Nacional de Pesquisas da Amazônia, Manaus 69067-375, AM, Brazil., Modolo LV; Departamento de Botânica, ICB, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; Department of Agricultural Biotechnology, Faculty of Agriculture, Ataturk University, Erzurum, Türkiye., Santos JCC; Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Maceió 57072-900, AL, Brazil; National Institute of Science and Technology in Bioanalytics Lauro Kubota (INCTBio-LK), Instituto de Química, Universidade Estadual de Campinas (Unicamp), PO Box 6154, Campinas 13083-970, SP, Brazil. Electronic address: josue@iqb.ufal.br., de Fátima Â; Departamento de Química, ICEx, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil; Department of Chemistry, Faculty of Science, Ataturk University, Erzurum, Türkiye. Electronic address: adefatima@qui.ufmg.br. |
| Πηγή: | Bioorganic chemistry [Bioorg Chem] 2026 Sep 05; Vol. 179, pp. 110009. Date of Electronic Publication: 2026 May 19. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: United States NLM ID: 1303703 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2120 (Electronic) Linking ISSN: 00452068 NLM ISO Abbreviation: Bioorg Chem Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Original Publication: New York, London, Academic Press. |
| Ιατρικοί όροι (MeSH): | Urease*/antagonists & inhibitors , Urease*/metabolism , Enzyme Inhibitors*/chemistry , Enzyme Inhibitors*/pharmacology , Enzyme Inhibitors*/chemical synthesis , Phosphines*/chemistry , Phosphines*/pharmacology , Phosphines*/chemical synthesis , Chalcogens*/chemistry , Chalcogens*/pharmacology , Soil*/chemistry, Canavalia/enzymology ; Structure-Activity Relationship ; Molecular Structure ; Dose-Response Relationship, Drug ; Kinetics |
| Περίληψη: | Urease, a metalloenzyme that catalyzes urea hydrolysis, is associated with nitrogen losses in soils. In agricultural settings, although the commercial inhibitor N-(butyl)thiophosphoric triamide (NBPT) is widely used, its low stability under adverse environmental conditions has prompted the search for new, more selective, and stable inhibitors. In this study, triphenylphosphine derivatives functionalized with chalcogens (SF1-SF3) and the corresponding precursor (SF4) were investigated for the ability to transiently inhibit urease catalytic activity. These compounds were tested against urease from Canavalia ensiformis and against soil with varying physicochemical properties. Classic kinetic assays and biophysical studies of urease-ligand interactions were carried out to investigate the mechanisms of urease inhibition. Even in the presence of humic substances, the selenium-containing derivative SF3 was the most effective urease inhibitor among the tested compounds, regardless of soil type. SF3 works as a typical uncompetitive inhibitor, likely by interacting with free cysteine residues located in the flap region near the active site. In situ spectroscopic evidence shows that SF3 may react with cysteine residues to form SF1 and H (Copyright © 2026 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Allosteric inhibition; Chalcogen-functionalized molecules; Selenium compounds; Urease inhibitors |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 0 (Enzyme Inhibitors) 0 (Phosphines) 0 (Chalcogens) 0 (Soil) FW6947296I (phosphine) |
| Entry Date(s): | Date Created: 20260523 Date Completed: 20260613 Latest Revision: 20260613 |
| Update Code: | 20260615 |
| DOI: | 10.1016/j.bioorg.2026.110009 |
| PMID: | 42176360 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1090-2120 |
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| DOI: | 10.1016/j.bioorg.2026.110009 |