Academic Journal
Light spectra shape population structure and the allocation of fucoxanthin and fatty acids with secondary phytohormone modulation in Tisochrysis lutea.
| Τίτλος: | Light spectra shape population structure and the allocation of fucoxanthin and fatty acids with secondary phytohormone modulation in Tisochrysis lutea. |
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| Συγγραφείς: | Kang S; Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea; Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea., Chansawang N; Biodiversity Research Centre, Thailand Institute of Scientific and Technological Research (TISTR), Pathum Thani 12120, Thailand., Kim KH; Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea., Chung N; Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea., Shin HH; Division of Fisheries Life Science, Pukyong National University, Busan 48574, Republic of Korea. Electronic address: shh961121@pknu.ac.kr., Li Z; Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea; Department of Bioresource and Environmental Engineering, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong, Daejeon 34113, Republic of Korea. Electronic address: lizhun@kribb.re.kr. |
| Πηγή: | Bioresource technology [Bioresour Technol] 2026 Oct; Vol. 457, pp. 134953. Date of Electronic Publication: 2026 May 22. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991- |
| Ιατρικοί όροι (MeSH): | Xanthophylls*/metabolism , Fatty Acids*/metabolism , Plant Growth Regulators*/pharmacology , Plant Growth Regulators*/metabolism , Light*, Photosynthesis/drug effects ; Photosynthesis/radiation effects ; Blue Light |
| Περίληψη: | Fucoxanthin and long-chain omega-3 fatty acids, particularly docosahexaenoic acid, are valuable microalgal products for nutraceutical and aquaculture applications. We investigated how light spectrum and phytohormone treatment affected growth, photosynthetic traits, population structure, and metabolite allocation in Tisochrysis lutea KCTC AG61399. Cultures were grown under white, red, or blue light-emitting diodes and four phytohormone regimes (control, abscisic acid, gibberellic acid, and indole-3-acetic acid) under continuous light at 100 μmol photons m-2 s-1. Growth, chlorophyll a, Fv/Fm, fucoxanthin, total fatty acids, fatty acid composition, and image-based single-cell cytometry profiles were quantified. Light spectrum was the dominant factor structuring the response. By day 8, red light produced the highest cell density (9.21 × 106 cells mL-1) and endpoint cell productivity, approximately 1.5-fold and 1.4-fold higher than under white and blue light, respectively. Red light also yielded the highest fucoxanthin content (1.43-1.62% DW), total fatty acid content (maximum 14.60% DW), and strongly single-cell-dominant populations (96.72-98.46%) with minimal aggregation. Auxiliary morphometric analysis showed that red-light cultures converged toward a smaller-cell, lower-biovolume single-cell state, whereas white and blue light retained larger dividing and aggregated fractions. Fatty acid class composition shifted with light spectrum, indicating altered lipid allocation and omega-3 partitioning rather than uniform expansion of the fatty acid pool. Phytohormones modified selected endpoints within each spectral regime but did not override the spectrum-driven pattern. These results show that biochemical measurements combined with event-based phenotyping improve interpretation of cultivation responses and support condition screening for coordinated fucoxanthin and fatty acid production in T. lutea. (Copyright © 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Tisochrysis lutea; fucoxanthin; image-based single-cell cytometry; light spectrum; omega-3 fatty acids; phytohormone |
| Substance Nomenclature: | 06O0TC0VSM (fucoxanthin) 0 (Xanthophylls) 0 (Fatty Acids) 0 (Plant Growth Regulators) |
| Entry Date(s): | Date Created: 20260522 Date Completed: 20260613 Latest Revision: 20260622 |
| Update Code: | 20260623 |
| DOI: | 10.1016/j.biortech.2026.134953 |
| PMID: | 42173372 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-2976 |
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| DOI: | 10.1016/j.biortech.2026.134953 |