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Biochemical characterization of a novel ochratoxin A-detoxifying amidohydrolase from Terribacillus saccharophilus JX-8 and its application in grape juice and wheat flour.

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Title: Biochemical characterization of a novel ochratoxin A-detoxifying amidohydrolase from Terribacillus saccharophilus JX-8 and its application in grape juice and wheat flour.
Authors: Liu B; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., Xiong M; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., Sun W; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., Wu H; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., Li J; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., Wang Y; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., Wang Q; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., He J; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address: hejian@njau.edu.cn., Chen X; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China. Electronic address: xuelanchen162@163.com.
Source: Food research international (Ottawa, Ont.) [Food Res Int] 2026 Aug 01; Vol. 237, pp. 119366. Date of Electronic Publication: 2026 Apr 30.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science Country of Publication: Canada NLM ID: 9210143 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7145 (Electronic) Linking ISSN: 09639969 NLM ISO Abbreviation: Food Res Int Subsets: MEDLINE
Imprint Name(s): Original Publication: Ottawa, Ontario, Canada : Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science, c1992-
MeSH Terms: Ochratoxins*/metabolism , Vitis*/chemistry , Vitis*/microbiology , Fruit and Vegetable Juices*/microbiology , Fruit and Vegetable Juices*/analysis , Triticum*/chemistry , Triticum*/microbiology , Flour*/analysis , Flour*/microbiology , Amidohydrolases*/metabolism , Amidohydrolases*/chemistry , Bacillaceae*/enzymology, Food Contamination/prevention & control ; Food Contamination/analysis ; Food Microbiology
Abstract: Ochratoxin A (OTA) is a widespread mycotoxin contaminating cereals, grape products, coffee, and spices, threatening global food safety and human health. Efficient detoxification strategies are therefore urgently needed. Herein, an OTA-degrading bacterium, Terribacillus saccharophilus JX-8, was isolated from Chinese sauerkraut juice, which completely degraded 12.4 μM OTA within 12 h via amide bond hydrolysis to yield non-toxic ochratoxin α (OTα). A novel OTA amidohydrolase OtaH was identified, sharing only 33%-42% sequence identity with previously characterized homologs. OtaH exhibited high catalytic efficiency (kcat/Km = 4028.57 mM-1 min-1) and remarkable thermostability, retaining >50% activity after incubation at 65 °C for 1 h. Moreover, 5 μg/mL OtaH achieved 64.9% and 94.6% degradation of OTA in grape juice and wheat flour, respectively, within 2 h at 25 °C, reducing residual OTA to 1.76 μg/L and 0.54 μg/kg. Notably, enzymatic treatment did not significantly alter pH, soluble solids content, titratable acidity, polyphenol content, or nutritional composition. This study provides a promising enzymatic strategy for OTA detoxification in food processing.
(Copyright © 2026 Elsevier Ltd. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Amidohydrolase; Biodegradation; Grape juice; Ochratoxin A; Terribacillus saccharophilus; Wheat flour
Substance Nomenclature: 0 (Ochratoxins)
1779SX6LUY (ochratoxin A)
EC 3.5.- (Amidohydrolases)
Entry Date(s): Date Created: 20260522 Date Completed: 20260717 Latest Revision: 20260717
Update Code: 20260717
DOI: 10.1016/j.foodres.2026.119366
PMID: 42169328
Database: MEDLINE
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  Data: Biochemical characterization of a novel ochratoxin A-detoxifying amidohydrolase from Terribacillus saccharophilus JX-8 and its application in grape juice and wheat flour.
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  Data: <searchLink fieldCode="AU" term="%22Liu+B%22">Liu B</searchLink>; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.<br /><searchLink fieldCode="AU" term="%22Xiong+M%22">Xiong M</searchLink>; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.<br /><searchLink fieldCode="AU" term="%22Sun+W%22">Sun W</searchLink>; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.<br /><searchLink fieldCode="AU" term="%22Wu+H%22">Wu H</searchLink>; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.<br /><searchLink fieldCode="AU" term="%22Li+J%22">Li J</searchLink>; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.<br /><searchLink fieldCode="AU" term="%22Wang+Y%22">Wang Y</searchLink>; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.<br /><searchLink fieldCode="AU" term="%22Wang+Q%22">Wang Q</searchLink>; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.<br /><searchLink fieldCode="AU" term="%22He+J%22">He J</searchLink>; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address: hejian@njau.edu.cn.<br /><searchLink fieldCode="AU" term="%22Chen+X%22">Chen X</searchLink>; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China. Electronic address: xuelanchen162@163.com.
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  Data: <i>Original Publication</i>: Ottawa, Ontario, Canada : Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science, c1992-
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  Data: <searchLink fieldCode="MM" term="%22Ochratoxins%22">Ochratoxins*</searchLink>/<searchLink fieldCode="MM" term="%22Ochratoxins+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Vitis%22">Vitis*</searchLink>/<searchLink fieldCode="MM" term="%22Vitis+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Vitis%22">Vitis*</searchLink>/<searchLink fieldCode="MM" term="%22Vitis+microbiology%22">microbiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Fruit+and+Vegetable+Juices%22">Fruit and Vegetable Juices*</searchLink>/<searchLink fieldCode="MM" term="%22Fruit+and+Vegetable+Juices+microbiology%22">microbiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Fruit+and+Vegetable+Juices%22">Fruit and Vegetable Juices*</searchLink>/<searchLink fieldCode="MM" term="%22Fruit+and+Vegetable+Juices+analysis%22">analysis</searchLink> <br /><searchLink fieldCode="MM" term="%22Triticum%22">Triticum*</searchLink>/<searchLink fieldCode="MM" term="%22Triticum+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Triticum%22">Triticum*</searchLink>/<searchLink fieldCode="MM" term="%22Triticum+microbiology%22">microbiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Flour%22">Flour*</searchLink>/<searchLink fieldCode="MM" term="%22Flour+analysis%22">analysis</searchLink> <br /><searchLink fieldCode="MM" term="%22Flour%22">Flour*</searchLink>/<searchLink fieldCode="MM" term="%22Flour+microbiology%22">microbiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Amidohydrolases%22">Amidohydrolases*</searchLink>/<searchLink fieldCode="MM" term="%22Amidohydrolases+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Amidohydrolases%22">Amidohydrolases*</searchLink>/<searchLink fieldCode="MM" term="%22Amidohydrolases+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillaceae%22">Bacillaceae*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillaceae+enzymology%22">enzymology</searchLink><br /><searchLink fieldCode="MH" term="%22Food+Contamination%22">Food Contamination</searchLink>/<searchLink fieldCode="MH" term="%22Food+Contamination+prevention+%26+control%22">prevention & control</searchLink> ; <searchLink fieldCode="MH" term="%22Food+Contamination%22">Food Contamination</searchLink>/<searchLink fieldCode="MH" term="%22Food+Contamination+analysis%22">analysis</searchLink> ; <searchLink fieldCode="MH" term="%22Food+Microbiology%22">Food Microbiology</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Ochratoxin A (OTA) is a widespread mycotoxin contaminating cereals, grape products, coffee, and spices, threatening global food safety and human health. Efficient detoxification strategies are therefore urgently needed. Herein, an OTA-degrading bacterium, Terribacillus saccharophilus JX-8, was isolated from Chinese sauerkraut juice, which completely degraded 12.4 μM OTA within 12 h via amide bond hydrolysis to yield non-toxic ochratoxin α (OTα). A novel OTA amidohydrolase OtaH was identified, sharing only 33%-42% sequence identity with previously characterized homologs. OtaH exhibited high catalytic efficiency (k<subscript>cat</subscript>/K<subscript>m</subscript> = 4028.57 mM<superscript>-1</superscript> min<superscript>-1</superscript>) and remarkable thermostability, retaining >50% activity after incubation at 65 °C for 1 h. Moreover, 5 μg/mL OtaH achieved 64.9% and 94.6% degradation of OTA in grape juice and wheat flour, respectively, within 2 h at 25 °C, reducing residual OTA to 1.76 μg/L and 0.54 μg/kg. Notably, enzymatic treatment did not significantly alter pH, soluble solids content, titratable acidity, polyphenol content, or nutritional composition. This study provides a promising enzymatic strategy for OTA detoxification in food processing.<br /> (Copyright © 2026 Elsevier Ltd. All rights reserved.)
– Name: Abstract
  Label: Competing Interests
  Group: Ab
  Data: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>Amidohydrolase; Biodegradation; Grape juice; Ochratoxin A; Terribacillus saccharophilus; Wheat flour
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 0 (Ochratoxins)<br />1779SX6LUY (ochratoxin A)<br />EC 3.5.- (Amidohydrolases)
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  Data: <i>Date Created: </i>20260522 <i>Date Completed: </i>20260717 <i>Latest Revision: </i>20260717
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        Value: 10.1016/j.foodres.2026.119366
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        Text: English
    PhysicalDescription:
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        StartPage: 119366
    Subjects:
      – SubjectFull: Food Contamination prevention & control
        Type: general
      – SubjectFull: Food Contamination analysis
        Type: general
      – SubjectFull: Food Microbiology
        Type: general
      – SubjectFull: Ochratoxins metabolism
        Type: general
      – SubjectFull: Vitis chemistry
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      – SubjectFull: Vitis microbiology
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      – SubjectFull: Fruit and Vegetable Juices microbiology
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      – SubjectFull: Fruit and Vegetable Juices analysis
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      – SubjectFull: Triticum chemistry
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      – SubjectFull: Triticum microbiology
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      – SubjectFull: Flour microbiology
        Type: general
      – SubjectFull: Amidohydrolases metabolism
        Type: general
      – SubjectFull: Amidohydrolases chemistry
        Type: general
      – SubjectFull: Bacillaceae enzymology
        Type: general
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      – TitleFull: Biochemical characterization of a novel ochratoxin A-detoxifying amidohydrolase from Terribacillus saccharophilus JX-8 and its application in grape juice and wheat flour.
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              Text: 2026 Aug 01
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