Academic Journal
Biochemical characterization of a novel ochratoxin A-detoxifying amidohydrolase from Terribacillus saccharophilus JX-8 and its application in grape juice and wheat flour.
| Τίτλος: | Biochemical characterization of a novel ochratoxin A-detoxifying amidohydrolase from Terribacillus saccharophilus JX-8 and its application in grape juice and wheat flour. |
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| Συγγραφείς: | Liu B; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., Xiong M; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., Sun W; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., Wu H; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., Li J; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., Wang Y; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., Wang Q; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China., He J; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address: hejian@njau.edu.cn., Chen X; College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, China. Electronic address: xuelanchen162@163.com. |
| Πηγή: | Food research international (Ottawa, Ont.) [Food Res Int] 2026 Aug 01; Vol. 237, pp. 119366. Date of Electronic Publication: 2026 Apr 30. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science Country of Publication: Canada NLM ID: 9210143 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7145 (Electronic) Linking ISSN: 09639969 NLM ISO Abbreviation: Food Res Int Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Ottawa, Ontario, Canada : Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science, c1992- |
| Ιατρικοί όροι (MeSH): | Ochratoxins*/metabolism , Vitis*/chemistry , Vitis*/microbiology , Fruit and Vegetable Juices*/microbiology , Fruit and Vegetable Juices*/analysis , Triticum*/chemistry , Triticum*/microbiology , Flour*/analysis , Flour*/microbiology , Amidohydrolases*/metabolism , Amidohydrolases*/chemistry , Bacillaceae*/enzymology, Food Contamination/prevention & control ; Food Contamination/analysis ; Food Microbiology |
| Περίληψη: | Ochratoxin A (OTA) is a widespread mycotoxin contaminating cereals, grape products, coffee, and spices, threatening global food safety and human health. Efficient detoxification strategies are therefore urgently needed. Herein, an OTA-degrading bacterium, Terribacillus saccharophilus JX-8, was isolated from Chinese sauerkraut juice, which completely degraded 12.4 μM OTA within 12 h via amide bond hydrolysis to yield non-toxic ochratoxin α (OTα). A novel OTA amidohydrolase OtaH was identified, sharing only 33%-42% sequence identity with previously characterized homologs. OtaH exhibited high catalytic efficiency (k (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Amidohydrolase; Biodegradation; Grape juice; Ochratoxin A; Terribacillus saccharophilus; Wheat flour |
| Substance Nomenclature: | 0 (Ochratoxins) 1779SX6LUY (ochratoxin A) EC 3.5.- (Amidohydrolases) |
| Entry Date(s): | Date Created: 20260522 Date Completed: 20260717 Latest Revision: 20260717 |
| Update Code: | 20260717 |
| DOI: | 10.1016/j.foodres.2026.119366 |
| PMID: | 42169328 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-7145 |
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| DOI: | 10.1016/j.foodres.2026.119366 |