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Electron Paramagnetic Resonance Spectroscopy of Spin-Labeled Lipids for Studying Lipid-Protein Interaction in Model and Biomembranes.

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Title: Electron Paramagnetic Resonance Spectroscopy of Spin-Labeled Lipids for Studying Lipid-Protein Interaction in Model and Biomembranes.
Authors: Páli T; Institute of Biophysics, HUN-REN Biological Research Centre, Szeged, Hungary. tpali@brc.hu., Kóta Z; Institute of Biophysics, HUN-REN Biological Research Centre, Szeged, Hungary.
Source: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 3001, pp. 555-588.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
Imprint Name(s): Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press
MeSH Terms: Membrane Proteins*/metabolism , Membrane Proteins*/chemistry , Lipids*/chemistry , Membrane Lipids*/chemistry , Membrane Lipids*/metabolism , Spin Labels*, Electron Spin Resonance Spectroscopy/methods ; Protein Binding
Abstract: Spin label electron paramagnetic resonance (EPR) of lipid-protein interactions reveals crucial features of the structure and assembly of integral membrane proteins. Spin label EPR spectroscopy is the technique of choice to characterize the protein-solvating lipid shell in its highly dynamic nature, because the EPR spectra of lipids that are spin-labeled close to the terminal methyl end of their acyl chains display two spectral components: those corresponding to lipids directly contacting the protein and those corresponding to lipids in the bulk fluid bilayer regions of the membrane. In this chapter, typical spin label EPR procedures are presented that allow the determination of the stoichiometry of interaction of spin-labeled lipids with the intramembranous region of membrane proteins or polypeptides, as well as the association constant of the spin-labeled lipid with respect to the host lipid. The experiments can be made on the most common, standard EPR spectrometers. The lipids giving rise to a so-called immobile spectral component in the EPR spectrum of such samples are identified as the motionally restricted first-shell lipids solvating membrane proteins in biomembranes. Stoichiometry and selectivity are directly related to the structure of the intramembranous sections of membrane-associated proteins or polypeptides [1] and can be used to study the state of assembly of such proteins in the membrane. Since these characteristics of lipid-protein interactions are discussed in detail in the literature (see [2, 3] for reviews), here we focus more on how to spin label model and biomembranes and how to measure and analyze the two-component EPR spectra of spin-labeled lipids in phospholipid bilayers that contain proteins or polypeptides. After a description of how to prepare spin-labeled model and native biological membranes, we present the reader with computational procedures for determining the molar fraction of motionally restricted lipids when both, one or none of the pure isolated-mobile or immobile-spectral components are available. With these topics, this chapter complements a previous methodological paper [4]. The interpretation of the data is discussed briefly, as well as other relevant and recent spin label EPR techniques for studying lipid-protein interactions, not only from the point of view of lipid chain dynamics.
(© 2026. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
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Contributed Indexing: Keywords: Annular, Solvation, Motionally restricted and bulk lipids; Electron paramagnetic resonance (EPR); Electron spin resonance (ESR); Immobile and mobile membrane lipids; Integral membrane proteins; Lipid–protein interactions; Phospholipids; Spin label; Spin-labeled; Two-component spectrum
Substance Nomenclature: 0 (Spin Labels)
0 (Membrane Proteins)
0 (Lipids)
0 (Membrane Lipids)
Entry Date(s): Date Created: 20260519 Date Completed: 20260717 Latest Revision: 20260717
Update Code: 20260717
DOI: 10.1007/978-1-0716-5054-7_22
PMID: 42156691
Database: MEDLINE
Description
ISSN:1940-6029
DOI:10.1007/978-1-0716-5054-7_22