Mapping recombination cold spots in wheat via meiotic recombination in a large biparental population.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Mapping recombination cold spots in wheat via meiotic recombination in a large biparental population.
Συγγραφείς:	Shaltouki-Rizi M; Department of Agronomy, Purdue University, 915 West State Street, West Lafayette, IN 47907, United States., Dewitt N; School of Plant, Environmental and Soil Sciences, Louisiana State University, 104 Sturgis Hall, Baton Rouge, LA 70803, United States., Mohammadi M; Department of Agronomy, Purdue University, 915 West State Street, West Lafayette, IN 47907, United States.
Πηγή:	G3 (Bethesda, Md.) [G3 (Bethesda)] 2026 Jul 06; Vol. 16 (7).
Τύπος έκδοσης:	Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Oxford University Press Country of Publication: England NLM ID: 101566598 Publication Model: Print Cited Medium: Internet ISSN: 2160-1836 (Electronic) Linking ISSN: 21601836 NLM ISO Abbreviation: G3 (Bethesda) Subsets: MEDLINE
Imprint Name(s):	Publication: 2021- : [Oxford] : Oxford University Press Original Publication: Bethesda, MD : Genetics Society of America, 2011-
Ιατρικοί όροι (MeSH):	Triticum/genetics , Meiosis/genetics , Chromosome Mapping* , Recombination, Genetic*, Chromosomes, Plant ; Polymorphism, Single Nucleotide ; Crossing Over, Genetic ; Genome, Plant ; Genetic Linkage ; Quantitative Trait Loci
Περίληψη:	Crossover (CO) recombination during meiosis is the fundamental driver of success in conventional plant breeding and a key determinant of genetic discoveries. Despite its importance, the fine-scale recombination landscape across the wheat genome remains partially characterized. Here, we constructed a dense genetic map by using 2,826 genotyping-by-sequencing SNP markers in a population of 345 F2:6 recombinant inbred lines, derived from a Penny x Yecora-Rojo cross. We constructed linkage maps spanning ∼3,507 cM across all 21 wheat chromosomes, and generated chromosome-wide recombination profiles and quantified local recombination rates in 5 Mb non-overlapping windows anchored to the reference genome. COs in wheat are overwhelmingly concentrated in distal and gene-rich regions, while the extensive pericentromeric cold spots span up to 77% of physical length. Low-recombination tracts (<0.1 cM/Mb) occupied 29.9 to 77.0% of chromosome physical length for all chromosomes. Average recombination rates ranged from 0.21 to 0.32 cM/Mb across the A subgenome, while local rates exceeded 5 cM/Mb in distal arms but dropped below 0.1 cM/Mb in centromeric regions. Chromosome 5A exhibited the longest genetic length (226 cM) but pronounced suppression in its subtelomeric and pericentromeric domains, whereas 4B showed the most extreme recombination desert, with 77% of its sequence nearly devoid of COs. These findings identified regions producing extended haplotype blocks and linkage drag in low-recombination regions that often constrain QTL resolution and limit access to allelic variation underlying key agronomic traits. The findings can also guide population design and inform strategies to redistribute COs through genetic, molecular, or epigenetic interventions by various genome rearrangement techniques in the future to unlock the hidden genetic diversity. (© The Author(s) 2026. Published by Oxford University Press on behalf of The Genetics Society of America.)
Grant Information:	College of Agriculture; Purdue University; the United States Department of Agriculture
Contributed Indexing:	Keywords: Marey map; RILs; cold spots; genetic map resources; genetic mapping; hot and cold recombination regions; linkage drag; recombination; trapped genetic variations; wheat
Entry Date(s):	Date Created: 20260519 Date Completed: 20260706 Latest Revision: 20260726
Update Code:	20260726
PubMed Central ID:	PMC13334167
DOI:	10.1093/g3journal/jkag097
PMID:	42154254
Βάση Δεδομένων:	MEDLINE

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ISSN:	2160-1836
DOI:	10.1093/g3journal/jkag097