Academic Journal
Development of a sheep model of mastitis caused by Mycoplasma bovis.
| Τίτλος: | Development of a sheep model of mastitis caused by Mycoplasma bovis. |
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| Συγγραφείς: | Wawegama NK; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address: nadeekaw@unimelb.edu.au., Kanci Condello A; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3010, Australia., Salgadu A; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3010, Australia., Elapathage D; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3010, Australia., Allen JL; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3010, Australia., Regmi S; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3010, Australia., Vaz PK; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3010, Australia., Sanaei R; Melbourne Veteinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3010, Australia., Dobson L; Melbourne Veteinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3010, Australia., Barber S; Melbourne Veteinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3010, Australia., Browning GF; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3010, Australia. |
| Πηγή: | Veterinary microbiology [Vet Microbiol] 2026 Aug; Vol. 319, pp. 111067. Date of Electronic Publication: 2026 May 09. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7705469 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2542 (Electronic) Linking ISSN: 03781135 NLM ISO Abbreviation: Vet Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam, Elsevier Scientific Pub. Co. |
| Ιατρικοί όροι (MeSH): | Mycoplasma bovis*/immunology , Mastitis*/microbiology , Mastitis*/veterinary , Mastitis*/pathology , Mycoplasma Infections*/veterinary , Mycoplasma Infections*/microbiology , Mycoplasma Infections*/pathology , Sheep Diseases*/microbiology , Sheep Diseases*/pathology , Disease Models, Animal*, Mammary Glands, Animal/microbiology ; Mammary Glands, Animal/pathology ; Milk/microbiology ; Milk/cytology ; Antibodies, Bacterial/blood ; Animals ; Female ; Sheep ; Cattle |
| Περίληψη: | Mycoplasma bovis (M. bovis) is an important pathogen of cattle, causing pneumonia, arthritis and mastitis. The study described here aimed to develop an ovine model of mastitis caused by M. bovis to facilitate studies of the pathogenesis of this disease. The left mammary glands of five lactating ewes were inoculated with M. bovis, and the left glands of another five lactating ewes were inoculated with culture medium. From day 2 after inoculation, milk production in the infected left glands dropped significantly, from an average of 250 mL/day to 40 mL/day. The mean somatic cell counts (SSC) of the milk from the infected glands were significantly higher than those of milk from the uninfected glands from day 1 to day 20 after inoculation, with the mean SCC of the milk from infected glands increasing from 64 cells/µL to > 4000 cells/µL. At necropsy on day 21 after inoculation, the infected glands were reduced in size, had lost most of the glandular tissue, and were filled with caseous material. Microscopically, the normal architecture of the mammary tissue was lost, with alveolar tissue and lobular ducts filled with inflammatory cells. Antibodies against M. bovis were detectable in the serum and milk of all the infected sheep by day 14 after inoculation. Thus, infection of the mammary gland of sheep with M. bovis induced mastitis typical of the naturally occurring disease associated with M. bovis in cattle, indicating that it could be used as a model to advance understanding of the pathogenesis of this disease. (Copyright © 2026 The Authors. Published by Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Nadeeka K Wawegama reports financial support was provided by Australian Research Council. Reports a relationship with that includes:. Has patent pending to. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Experimental infection; Infection model; Mammary infection; Mastitis; Mycoplasma bovis; Mycoplasma mastitis; Sheep |
| Substance Nomenclature: | 0 (Antibodies, Bacterial) |
| Entry Date(s): | Date Created: 20260516 Date Completed: 20260603 Latest Revision: 20260603 |
| Update Code: | 20260604 |
| DOI: | 10.1016/j.vetmic.2026.111067 |
| PMID: | 42142483 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-2542 |
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| DOI: | 10.1016/j.vetmic.2026.111067 |