Academic Journal
Passive liquefaction and colloidal centrifugation demonstrate improved semen quality following liquefaction of semen collected in the bonobo (Pan paniscus).
| Τίτλος: | Passive liquefaction and colloidal centrifugation demonstrate improved semen quality following liquefaction of semen collected in the bonobo (Pan paniscus). |
|---|---|
| Συγγραφείς: | Dziubinski M; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA., Olds J; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA., Stricklin OE; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA., Skiba SA; Ape Cognition and Conservation Initiative (Ape Initiative), Des Moines, IA., Beachler TM; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA. |
| Πηγή: | American journal of veterinary research [Am J Vet Res] 2026 May 15; Vol. 87 (8). Date of Electronic Publication: 2026 May 15 (Print Publication: 2026). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Veterinary Medical Association Country of Publication: United States NLM ID: 0375011 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1943-5681 (Electronic) Linking ISSN: 00029645 NLM ISO Abbreviation: Am J Vet Res Subsets: MEDLINE |
| Imprint Name(s): | Publication: Schaumburg, Ill. : American Veterinary Medical Association Original Publication: Chicago : American Veterinary Medical Assn. |
| Ιατρικοί όροι (MeSH): | Semen*/physiology , Semen Analysis*/veterinary , Pan paniscus*/physiology, Centrifugation/veterinary ; Centrifugation/methods ; Spermatozoa/physiology ; Animals ; Male ; Sperm Motility ; Cross-Sectional Studies ; Colloids |
| Περίληψη: | Objective: To evaluate different processing methods on the liquefaction of the coagulum from semen collected in the bonobo (Pan paniscus). Methods: In this experimental cross-sectional study, ejaculates voluntarily collected were divided among 4 processing methods and evaluated for subjective total and progressive motility, numbers of total and progressively motile spermatozoa per ejaculate, and morphology. Data were assessed for normality using a Shapiro-Wilk test and subsequently tested for between-group differences using a Kruskal-Wallis test, with significance set at P < .05. Results: A total of 22 ejaculates collected from 3 males were divided among 4 groups: passive liquefaction (PL; n = 6), HEPES-balanced salt solution (4), trypsin enzymatic digestion (6), and colloidal centrifugation (CC; 6). The PL and CC groups showed the highest subjective mean total (43.3 ± 31.25% and 51.7 ± 32.81%) and progressive motility (35.0 ± 28.28% and 45.8 ± 30.07%), both of which were significantly greater than the HEPES-balanced salt solution group (0%). No method significantly surpassed the others in either the total or the progressively motile number of spermatozoa. The PL and CC groups also showed significantly higher percentages of normal morphology (60.67 ± 9.65% and 58.67 ± 13.97%, respectively) compared to the trypsin enzymatic digestion group (30.5 ± 8.87%). Conclusions: PL and CC processing methods produced ejaculates with the highest subjective total and progressive motility and normal morphology upon microscopic evaluation. Clinical Relevance: This study suggests that the processing methods of passive liquefaction and colloidal centrifugation may be most efficacious for preserving bonobo spermatozoa motility and morphology. |
| Contributed Indexing: | Keywords: bonobo; coagulum; colloidal centrifugation; passive liquefaction; semen |
| Substance Nomenclature: | 0 (Colloids) |
| Entry Date(s): | Date Created: 20260515 Date Completed: 20260731 Latest Revision: 20260731 |
| Update Code: | 20260801 |
| DOI: | 10.2460/ajvr.26.03.0101 |
| PMID: | 42140278 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1943-5681 |
|---|---|
| DOI: | 10.2460/ajvr.26.03.0101 |