Academic Journal
In-syringe solid-phase extraction coupled with UPLC-MS/MS for simultaneous determination of reducing sugars, animo acids and Amadori compounds in tobacco.
| Title: | In-syringe solid-phase extraction coupled with UPLC-MS/MS for simultaneous determination of reducing sugars, animo acids and Amadori compounds in tobacco. |
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| Authors: | Wang X; School of Chemistry, Sun Yat-sen University, Guangzhou, 510006, China., Lai Y; Technology Center, China Tobacco Guangdong Industrial Co., Ltd., Guangzhou 510385, China., Guo S; Technology Center, China Tobacco Guangdong Industrial Co., Ltd., Guangzhou 510385, China., Qin H; School of Chemistry, Sun Yat-sen University, Guangzhou, 510006, China., Liu Z; School of Chemistry, Sun Yat-sen University, Guangzhou, 510006, China., Xia L; School of Chemistry, Sun Yat-sen University, Guangzhou, 510006, China., Wang X; Technology Center, China Tobacco Guangdong Industrial Co., Ltd., Guangzhou 510385, China., Wu J; Technology Center, China Tobacco Guangdong Industrial Co., Ltd., Guangzhou 510385, China. Electronic address: wujunzhang@gdzygy.com., Li G; School of Chemistry, Sun Yat-sen University, Guangzhou, 510006, China. Electronic address: cesgkl@mail.sysu.edu.cn. |
| Source: | Journal of chromatography. A [J Chromatogr A] 2026 Aug 16; Vol. 1781, pp. 467068. Date of Electronic Publication: 2026 May 05. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9318488 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3778 (Electronic) Linking ISSN: 00219673 NLM ISO Abbreviation: J Chromatogr A Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam ; New York : Elsevier, 1993- |
| MeSH Terms: | Tandem Mass Spectrometry*/methods , Solid Phase Extraction*/methods , Solid Phase Extraction*/instrumentation , Nicotiana*/chemistry , Liquid Chromatography-Mass Spectrometry*/methods , Carbohydrates*/analysis , Carbohydrates*/isolation & purification , Carbohydrates*/chemistry , Amino Acids*/analysis , Amino Acids*/isolation & purification , Sugars*/analysis , Sugars*/isolation & purification, Chromatography, High Pressure Liquid/methods ; Reproducibility of Results ; Limit of Detection ; Linear Models ; Maillard Reaction |
| Abstract: | Considering the critical role of Maillard reaction-related compounds in determining the sensory and chemical stability of tobacco, this study develops an in-syringe SPE coupled with UPLC-MS/MS method for simultaneous determination of 43 Maillard reaction-related compounds in tobacco, covering reducing sugars (3), amino acids (17), Amadori compounds (17), and structurally related intermediates (6). The sample pretreatment utilizes HLB-packed self-assembled columns for SPE, followed by methanol elution. The extracted solution is then analyzed using a Titank C (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Amadori compounds; In-syringe solid-phase extraction; Maillard reaction; Tobacco analysis |
| Substance Nomenclature: | 0 (Carbohydrates) 0 (Amino Acids) 0 (Sugars) |
| Entry Date(s): | Date Created: 20260515 Date Completed: 20260716 Latest Revision: 20260716 |
| Update Code: | 20260717 |
| DOI: | 10.1016/j.chroma.2026.467068 |
| PMID: | 42140040 |
| Database: | MEDLINE |
| ISSN: | 1873-3778 |
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| DOI: | 10.1016/j.chroma.2026.467068 |