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Characterization of functional metabolites in broccoli by-product using green extraction with supercritical fluids.

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Τίτλος: Characterization of functional metabolites in broccoli by-product using green extraction with supercritical fluids.
Συγγραφείς: Gudiño I; Nutrición y Bromatología, Instituto Universitario de Investigación en Recursos Agrarios (INURA), Escuela de Ingenierías Agrarias, Universidad de Extremadura, Badajoz, Spain., Casquete R; Nutrición y Bromatología, Instituto Universitario de Investigación en Recursos Agrarios (INURA), Escuela de Ingenierías Agrarias, Universidad de Extremadura, Badajoz, Spain., Benito MJ; Nutrición y Bromatología, Instituto Universitario de Investigación en Recursos Agrarios (INURA), Escuela de Ingenierías Agrarias, Universidad de Extremadura, Badajoz, Spain., Córdoba MG; Nutrición y Bromatología, Instituto Universitario de Investigación en Recursos Agrarios (INURA), Escuela de Ingenierías Agrarias, Universidad de Extremadura, Badajoz, Spain., Prieto MH; Instituto de Investigaciones Agrarias. Finca La Orden-Valdesequera. CICYTEX (Junta de Extremadura), Badajoz, Spain., Martín A; Nutrición y Bromatología, Instituto Universitario de Investigación en Recursos Agrarios (INURA), Escuela de Ingenierías Agrarias, Universidad de Extremadura, Badajoz, Spain.
Πηγή: Journal of the science of food and agriculture [J Sci Food Agric] 2026 Aug 30; Vol. 106 (11), pp. 6789-6799. Date of Electronic Publication: 2026 May 13.
Τύπος έκδοσης: Journal Article; Evaluation Study
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: John Wiley & Sons Country of Publication: England NLM ID: 0376334 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0010 (Electronic) Linking ISSN: 00225142 NLM ISO Abbreviation: J Sci Food Agric Subsets: MEDLINE
Imprint Name(s): Publication: <2005-> : Chichester, West Sussex : John Wiley & Sons
Original Publication: London, Society of Chemical Industry.
Ιατρικοί όροι (MeSH): Brassica*/chemistry , Brassica*/metabolism , Brassica*/growth & development , Chromatography, Supercritical Fluid*/methods , Plant Extracts*/isolation & purification , Plant Extracts*/chemistry , Plant Extracts*/pharmacology , Plant Extracts*/metabolism , Waste Products*/analysis, Antioxidants/isolation & purification ; Antioxidants/chemistry ; Antioxidants/pharmacology ; Antioxidants/metabolism ; Phenols/chemistry ; Phenols/isolation & purification ; Phenols/pharmacology ; Phenols/metabolism ; Plant Leaves/chemistry ; Plant Leaves/metabolism ; Anti-Bacterial Agents/isolation & purification ; Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/metabolism ; Bacillus cereus/drug effects ; Bacillus cereus/growth & development ; Plant Stems/chemistry ; Chromatography, High Pressure Liquid
Περίληψη: Background: The present study aimed to valorize broccoli by-products (leaves and stems) through the extraction of bioactive compounds using supercritical fluid extraction (SFE). The influence of broccoli variety, plant part and maturation stage on total phenolic content (TPC), antioxidant activity and antimicrobial properties was evaluated. Additionally, the bioactive compounds present in the extracts were identified by HPLC-UV-electrospray ionization-tandem mass spectrometry.
Results: Although extraction yields were lower than those obtained with conventional methods, SFE extracts exhibited high antioxidant activity, reaching values up to 1311.8 mg Trolox kg-1 of dry extract in the CLX 3570 variety using the DPPH assay. A strong correlation between phenolic content and antioxidant activity was observed. The extracts also showed notable antimicrobial activity, with inhibition values up to 98.09% against Bacillus cereus, particularly in the Shard cultivar. This effect was associated with the presence of specific fatty acids, such as linoleic and palmitic acids, which were more abundant in stems. Furthermore, increasing maturation stage significantly enhanced extraction yield, TPC, antioxidant activity and antimicrobial capacity.
Conclusion: Broccoli by-products represent a promising source of functional bioactive compounds. SFE emerges as a sustainable extraction technique capable of producing high-quality extracts with significant biological activity, contributing to waste valorization and the development of functional ingredients for the food industry. © 2026 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
(© 2026 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.)
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Grant Information: Junta de Extremadura and Feder Funds
Contributed Indexing: Keywords: Brassica; HPLC‐UV‐ESI‐MS/MS; antimicrobial activity; antioxidant activity; bioactive compounds; by‐products; supercritical fluid extraction
Substance Nomenclature: 0 (Plant Extracts)
0 (Antioxidants)
0 (Phenols)
0 (Waste Products)
0 (Anti-Bacterial Agents)
Entry Date(s): Date Created: 20260514 Date Completed: 20260708 Latest Revision: 20260726
Update Code: 20260726
PubMed Central ID: PMC13341030
DOI: 10.1002/jsfa.70718
PMID: 42130266
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1097-0010
DOI:10.1002/jsfa.70718