Academic Journal
Impact of repeated ovulation induction and superovulation on ovarian reserve and early embryo development in obese and PCOS-like mouse models.
| Τίτλος: | Impact of repeated ovulation induction and superovulation on ovarian reserve and early embryo development in obese and PCOS-like mouse models. |
|---|---|
| Συγγραφείς: | Kunhiraman JP; Division of Reproductive Biology, Department of Reproductive Science, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka 576104, India., Dcunha R; Sree Mookambika Centre for Research and Innovation, Sree Mookambika Institutes, Padanilam, Kulasekharam, Tamil Nadu 629161, India., Madhvacharya VV; Division of Reproductive Biology, Department of Reproductive Science, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka 576104, India., R VL; Department of Health Technology and Informatics, Prasanna School of Public Health, Manipal Academy of Higher Education, Manipal, Karnataka 576104, India., Venkatraman G; School of Biosciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu 632014, India., Shah NR; Manipal Institute of Regenerative Medicine, Manipal Academy of Higher Education, Manipal, Karnataka 576104, India., Kumar A; Manipal Institute of Regenerative Medicine, Manipal Academy of Higher Education, Manipal, Karnataka 576104, India., Adiga SK; Centre of Excellence in Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka 576104, India., Kannan N; Division of Experimental Pathology and Laboratory Medicine, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.; Center for Regenerative Medicine, Mayo Clinic, Rochester, MN 55905, USA.; Mayo Clinic Cancer Center, Mayo Clinic, Rochester, MN 55905, USA., Kalthur G; Division of Reproductive Biology, Department of Reproductive Science, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka 576104, India.; Sree Mookambika Centre for Research and Innovation, Sree Mookambika Institutes, Padanilam, Kulasekharam, Tamil Nadu 629161, India. |
| Πηγή: | Reproduction, fertility, and development [Reprod Fertil Dev] 2026 May 29; Vol. 38 (8). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Commonwealth Scientific and Industrial Research Organisation Country of Publication: Australia NLM ID: 8907465 Publication Model: Print Cited Medium: Internet ISSN: 1448-5990 (Electronic) Linking ISSN: 10313613 NLM ISO Abbreviation: Reprod Fertil Dev Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: East Melbourne, Vic., Australia : Commonwealth Scientific and Industrial Research Organisation, c1989- |
| Ιατρικοί όροι (MeSH): | Superovulation*/drug effects , Obesity*/physiopathology , Obesity*/complications , Ovulation Induction*/adverse effects , Ovulation Induction*/methods , Embryonic Development*/drug effects , Embryonic Development*/physiology , Ovarian Reserve*/drug effects , Ovarian Reserve*/physiology , Polycystic Ovary Syndrome*/physiopathology , Polycystic Ovary Syndrome*/complications, Gonadotropins, Equine/administration & dosage ; Chorionic Gonadotropin/pharmacology ; Animals ; Female ; Mice ; Disease Models, Animal ; Pregnancy ; Diet, High-Fat |
| Περίληψη: | Context: Women with obesity or polycystic ovary syndrome (PCOS) often require repeated ovulation induction (OI) due to ovulatory dysfunction and infertility. However, the cumulative effects of repeated stimulation on ovarian function and embryo development remain poorly understood. Aims: The present study aimed to understand the consequences of repeated OI and/or repeated superovulation (SO) on preimplantation embryonic development in obese (high-fat diet-induced) and PCOS (high-fat diet and dehydroepiandrosterone-induced) mouse models. Methods: Control (without any metabolic disorder), obese, and PCOS mice were divided into three experimental groups. The mice in one round of superovulation (SO1) were injected with 5 IU intraperitoneal (i.p.) injection of pregnant mare serum gonadotropin, followed by 10 IU of human chorionic gonadotropin 48 h later. In the repeated superovulation (SO3) group, mice were subjected to three rounds of superovulation with a gap of 9 days between each superovulation. Mice in the OI3 + SO3 group were administered with three doses of CC (50 mg/kg body weight, i.p., gap of 9 days between each CC injection), followed by three rounds of superovulation. Key Results: Both single and three rounds of superovulation resulted in decrease in primordial follicles and a moderate increase in atretic follicles in obese and PCOS mice. Reduction in the number of ovulated oocytes, increased rate of abnormal fertilization, decrease in blastocyst rate and elevated DNA damage in blastocysts were observed in in vitro fertilization-derived embryos. Conclusions: These findings demonstrate compromised embryonic development following repeated ovarian stimulation. Implications: The study highlights need for mild ovarian stimulation strategies in metabolically compromised patients undergoing infertility treatments. (© 2026 The Author(s) (or their employer(s)). Published by CSIRO Publishing.) |
| Contributed Indexing: | Keywords: DNA damage; clomiphene citrate; follicular atresia; gonadotrophins; in vitro fertilization; infertility; oocyte–cumulus complex; polyspermic fertilization |
| Substance Nomenclature: | 0 (Gonadotropins, Equine) 0 (Chorionic Gonadotropin) |
| Entry Date(s): | Date Created: 20260513 Date Completed: 20260716 Latest Revision: 20260716 |
| Update Code: | 20260717 |
| DOI: | 10.1071/RD26037 |
| PMID: | 42128672 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1448-5990 |
|---|---|
| DOI: | 10.1071/RD26037 |