Academic Journal
Phenotypic and whole-genome characterization of a novel Lysinibacillus fusiformis PWR01 isolated from rubber latex with multifunctional probiotic and antimicrobial potential.
| Τίτλος: | Phenotypic and whole-genome characterization of a novel Lysinibacillus fusiformis PWR01 isolated from rubber latex with multifunctional probiotic and antimicrobial potential. |
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| Συγγραφείς: | Permpoonpattana P; Department of Agricultural Science and Technology, Faculty of Innovative Agriculture, Fisheries and Food, Prince of Songkla University, Surat Thani, 84000, Thailand., Chakma A; BioMac Lab, Dhaka, Bangladesh. Electronic address: ashim3041@gmail.com., Mwamburi SM; Department of Fisheries, Kenya Marine and Fisheries Research Institute, P.O. Box 81651-80100, Mombasa, Kenya., Khang LTP; Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand., Sihamok W; Department of Agricultural Science and Technology, Faculty of Innovative Agriculture, Fisheries and Food, Prince of Songkla University, Surat Thani, 84000, Thailand., Phetduang K; Division of Biotechnology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, 50100, Thailand., Therdtatha P; Division of Biotechnology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, 50100, Thailand., Islam SI; Canadian River Institute, Department of Biology, University of Prince Edward Island, Canada. Electronic address: sislam19880@upei.ca., Amin N; BioMac Lab, Dhaka, Bangladesh. Electronic address: nusramin14@gmail.com., Shahed K; BioMac Lab, Dhaka, Bangladesh. Electronic address: khandkershahed23@gmail.com., Sangsawad P; School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand. Electronic address: papungkorn@sut.ac.th., Linh NV; Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand; Center of Omics for High-Value Agriculture (AgOmicsCMU), Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand. Electronic address: linhvu.n@cmu.ac.th. |
| Πηγή: | Microbial pathogenesis [Microb Pathog] 2026 Jul; Vol. 216, pp. 108546. Date of Electronic Publication: 2026 May 09. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London ; Orlando : Academic Press, c1986- |
| Ιατρικοί όροι (MeSH): | Bacillaceae*/genetics , Bacillaceae*/isolation & purification , Bacillaceae*/classification , Bacillaceae*/physiology , Genome, Bacterial*/genetics , Anti-Infective Agents*/pharmacology , Probiotics* , Latex* , Rubber*, Biofilms/growth & development ; Biofilms/drug effects ; Spores, Bacterial/growth & development ; Phenotype ; Phylogeny ; Whole Genome Sequencing ; Hydrogen-Ion Concentration ; Microbial Viability ; Antioxidants ; Antibiosis |
| Περίληψη: | Microbial resources from plant-derived environments harbor a reservoir of functionally diverse probiotic candidates, yet information on the probiotic potential of Lysinibacillus species from rubber latex environment remains limited. This study aimed to characterize Lysinibacillus sp. PWR01, isolated from rubber latex, through comprehensive integrated phenotypic, functional, and genomic analyses to evaluate its biotechnological potential. Morphological, biochemical, and physiological assays were combined with whole-genome sequencing, comparative genomics, and genome functional annotation. PWR01 demonstrated strong tolerance to acidic pH and bile salt conditions, high sporulation efficiency, and pronounced antibiofilm activity against Pseudomonas aeruginosa. In addition, the cell-free supernatant inhibited Streptococcus agalactiae and Aeromonas hydrophila in a concentration-dependent manner across a range of temperature and pH conditions. The strain also exhibited notable antioxidant activity and maintained high cell viability during storage. Genome analysis revealed a 5.0 Mb genome with 4909 predicted genes and 97% average nucleotide identity (ANI) with Lysinibacillus fusiformis. Functional annotation identified genes associated with sporulation, stress tolerance, carbohydrate metabolism, and secondary metabolite biosynthesis, including polyketide and nonribosomal peptide biosynthetic pathways. Despite the presence of antimicrobial resistance-associated genes, no corresponding phenotypic antimicrobial resistance was detected. These findings collectively highlight Lysinibacillus fusiformis PWR01 as a metabolically versatile and resilient strain with considerable probiotic and biotechnological potential. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Comparative genomics; Lysinibacillus; Probiotic potential; Rubber latex; Sporulation |
| Substance Nomenclature: | 0 (Latex) 0 (Anti-Infective Agents) 9006-04-6 (Rubber) 0 (Antioxidants) |
| Entry Date(s): | Date Created: 20260511 Date Completed: 20260716 Latest Revision: 20260716 |
| Update Code: | 20260717 |
| DOI: | 10.1016/j.micpath.2026.108546 |
| PMID: | 42114786 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1096-1208 |
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| DOI: | 10.1016/j.micpath.2026.108546 |