Academic Journal
Simple and high-efficiency cotton fiber-in-syringe solid-phase extraction of extracellular vesicles.
| Title: | Simple and high-efficiency cotton fiber-in-syringe solid-phase extraction of extracellular vesicles. |
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| Authors: | Chen M; College of Pharmaceutical Sciences, Soochow University, Suzhou, 215000, China., Tang Y; College of Pharmaceutical Sciences, Soochow University, Suzhou, 215000, China., Jiang Z; Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, 710127, China., Zhang J; Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, 710127, China., Zhang H; College of Pharmaceutical Sciences, Soochow University, Suzhou, 215000, China. Electronic address: hyzhang2020@suda.edu.cn., Wei Y; Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, 710127, China. Electronic address: ymwei@nwu.edu.cn. |
| Source: | Analytica chimica acta [Anal Chim Acta] 2026 Jul 15; Vol. 1407, pp. 345548. Date of Electronic Publication: 2026 Apr 17. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370534 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4324 (Electronic) Linking ISSN: 00032670 NLM ISO Abbreviation: Anal Chim Acta Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Original Publication: Amsterdam. |
| MeSH Terms: | Solid Phase Extraction*/instrumentation , Solid Phase Extraction*/methods , Extracellular Vesicles*/chemistry , Cotton Fiber*, Humans |
| Abstract: | Extracellular vesicles (EVs) are promising to provide chemical predictors toward clinical diagnosis and prognosis. However, the isolation of high-purity EVs from complex bodily fluids is a little complex. To address this issue, a choline phosphate (CP)-functionalized adsorbent, designated as SCF@CP-Et, was prepared by grafting 2-(isopropylacryloyloxy)ethyl choline phosphate (CP-Et) from the surface of sulphydryl cotton fiber (SCF) via thiol-ene click polymerization. The SCF@CP-Et indicates a high-efficiency EV enrichment capability arising from the multivalent coordination interaction between the CP groups and phosphatidylcholine (PC) on EV membrane surface, meanwhile possesses an excellent anti-protein adsorption performance derived from the abundant hydroxyl groups of cotton fiber and zwitterionic property of CP-Et. Besides, this cotton fiber is easily assembled into a cotton fiber-in-syringe solid-phase extraction (SPE) device. This device shows simple and convenient operation during the isolation of EVs from various samples, including cell culture medium, plasma and urine. Meanwhile, the released EVs can maintain their biological activity. Taking advantage of these properties, this method was employed to isolate EVs in the plasma of colorectal cancer (CRC) patients and healthy donors, and 69 up-regulated and 18 down-regulated proteins are identified by proteomics analysis. Most of these up-regulated proteins, such as TLNA, FLNA, ITGB3 and MYH9, are associated with the progression of CRC, demonstrating the potential of this carbon fiber-based SPE method in the EV-based early diagnosis of diseases. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Absorbent; Cotton fiber; Extracellular vesicles; Proteomics; Solid-phase extraction |
| Entry Date(s): | Date Created: 20260510 Date Completed: 20260716 Latest Revision: 20260731 |
| Update Code: | 20260731 |
| DOI: | 10.1016/j.aca.2026.345548 |
| PMID: | 42108049 |
| Database: | MEDLINE |
| ISSN: | 1873-4324 |
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| DOI: | 10.1016/j.aca.2026.345548 |